Plasmonically Enhanced Point-of-care Detection of Cardiac Biomarkers by a Smart Phone
通过智能手机对心脏生物标志物进行等离子体增强即时检测
基本信息
- 批准号:10570910
- 负责人:
- 金额:$ 66.58万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-02-01 至 2025-01-31
- 项目状态:未结题
- 来源:
- 关键词:AcuteAdsorptionAntibodiesArchitectureBiological AssayBiological MarkersBloodCapillarityCardiacCardiovascular DiseasesCardiovascular systemCause of DeathCellular PhoneCessation of lifeChest PainChickensClinicalClinical MedicineClinical ResearchCongestive Heart FailureCustomDetectionDevelopmentDevicesDiagnosisDropsElectromagnetic FieldsElectronsEngineeringEnzyme-Linked Immunosorbent AssayFilmFluorescenceGenerationsGeometryHeart DiseasesHomeHospitalsHumanImageImaging DeviceImmunoassayIndividualInfrastructureInpatientsLaboratoriesMetalsMicrofluidic MicrochipsMicrofluidicsMonitorMyocardial InfarctionNanostructuresNoiseOutputPatientsPerformancePlayPrimary PreventionPrintingProtein MicrochipsProteinsReaderReagentRefrigerationResearchRoleSamplingSecondary PreventionSerumSignal TransductionSilverSpottingsSurfaceTechniquesTelephoneTestingThickUnited StatesUniversity HospitalsVariantabsorptionantibody detectionclinical decision-makingcost effectivecross reactivitydesigndetection limitdetectorexpectationfabricationfetal bovine serumfluorescence imagingfluorophoreinnovationinsightnanonanoscalenovelplasmonicspoint of carepoint of care testingpoint-of-care detectionportabilitypro-brain natriuretic peptide (1-76)risk stratificationself testingstemtechnological innovationtreatment responseuser-friendlyvirtualwireless transmission
项目摘要
ABSTRACT
Heart disease is the leading cause of death in the United States, responsible for more than 630,000 deaths
annually. Cardiovascular biomarkers play a fundamental role in the diagnosis, risk stratification, and treatment
of a wide variety of cardiovascular disorders. Currently there are several testing devices and techniques to
quantify the levels of cardiac biomarkers; however, these assays are designed exclusively for clinical settings
and are engineered for the detection of individual biomarkers. The objective of this project is to develop an
inkjet-printed, plasmonically enhanced, single-step, multiplex point-of-care test for the quantification of key
cardiovascular biomarkers, specifically NT-proBNP, cTnT, and CRP. The proposed biomarkers are central to
the diagnosis and management of a wide spectrum of cardiovascular conditions, including acute chest pain
and myocardial infarction, acute and chronic heart failure, and primary and secondary prevention. The
technological innovation of this project stems from the integration of two state-of-the-art platforms—1) a single
step, multiplex point-of-care protein microarray with high sensitivity and low background noise and 2) a
plasmonic nanostructure that has shown four orders of magnitude fluorescence enhancements—to enable
testing from a drop of blood using a cellphone-based fluorescence imaging device in a hospital or home
setting. In this project, all components of the plasmonically enhanced assay will be optimized, a smart phone-
compatible multiplex chip will be integrated into a passive, capillarity-driven microfluidics device and its figures-
of-merit evaluated using archival patient samples. Finally, we will conduct a clinical study where we will
quantify the levels of NTpro-BNP, cTnT, and CRP simultaneously from a single drop of blood in approximately
90 patients, employing our point-of-care assay platform, which will be compared to results from commercial
immunoassay analyzers at Duke University Hospital. The proposed project is expected to lead to an
inexpensive point-of-care cardiac panel, which will have major impact on the diagnosis and treatment of
cardiovascular disorders, as detection of multiple biomarkers provides deeper insight into the underlying
pathophysiological stages, and self-testing by patients at home enables closer monitoring of treatment
response. Moreover, this assay platform can target any analyte for which antibody reagents are available,
making it broadly applicable to most, if not all, immunoassay targets in clinical medicine.
摘要
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Maiken Mikkelsen其他文献
Maiken Mikkelsen的其他文献
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{{ truncateString('Maiken Mikkelsen', 18)}}的其他基金
Plasmonically Enhanced Point-of-care Detection of Cardiac Biomarkers by a Smart Phone
通过智能手机对心脏生物标志物进行等离子体增强即时检测
- 批准号:
10358496 - 财政年份:2019
- 资助金额:
$ 66.58万 - 项目类别:
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