Pathology

病理

• 批准号: 10571810
• 负责人: Michael H. A. Roehrl
• 金额: $ 76.36万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-20 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10571810
• 关键词: Antibodies; Basic Cancer Research; Biological; Blood specimen; Cancer Center Support Grant; Cells; Clinical; Collection; DNA; Data; Detection; Formalin; Freezing; Funding; Histologic; Histology; Human; Human Resources; Immunohistochemistry; Institution; Journals; Laboratories; Liquid substance; Malignant Neoplasms; Manuals; Molecular; Monoclonal Antibodies; Paper; Paraffin Embedding; Pathology; Population; Prevention; Procedures; Process; Proteins; Protocols documentation; Publications; Publishing; RNA; Research; Research Personnel; Resources; Sampling; Services; Source; Specimen; Stains; The Cancer Genome Atlas; Tissue Embedding; Tissue Microarray; Tissues; Work; anticancer research; biobank; cancer therapy; cost effective; design; detection platform; experimental study; human tissue; interest; laser capture microdissection; neoplastic; neoplastic cell; new technology; polyclonal antibody; programs; proteogenomics; research study; technology platform; tissue resource; translational cancer research; tumor

ABSTRACT The Pathology Core is an integral pillar of the MSK Precision Pathology Biobanking Center and provides a comprehensive resource for human tissue-based research that takes advantage of the unique tissue resources available at MSK. The acquisition and banking of human biologic specimens to be used to study causes, detection, prevention, and treatment of cancer have become an indispensible resource for basic and translational cancer researchers throughout MSK. The reliability of molecular data derived from new technology platforms depends on access to an adequate supply of optimally procured, high-quality tissue specimens. The Pathology Core at MSK provides efficient and cost-effective services to facilitate tissue use and to conduct human biospecimen-based experimentation. The Core also serves as a national resource, as evidenced by the over 1,000 samples from multiple tumor types that have been provided to the NCI-funded TCGA initiative, as well as the samples that will be contributed to the NCI’s CPTAC proteogenomics consortium. The Histology Service within the Core facilitates selection of appropriate specimens for tissue analyses and provides the basic services of cutting, dissecting, and staining of fresh-frozen and formalin-fixed, paraffin- embedded tissues. It includes construction of tissue microarray blocks and laser capture microdissection for isolation of pure cell populations and tumor cell enrichment. The Core’s Immunohistochemistry Service provides automated and manual staining using optimized monoclonal and polyclonal antibodies to be used in clinico-pathologic research studies. The service also continuously develops and optimizes protocols for new antibodies and for a variety of sensitive detection systems. The services and collaborative work provided by the Pathology Core have contributed to 1,685 publications of MSK researchers from nine of the 10 Center Programs. Many of these papers were published in leading cancer and general interest journals.

抽象的 病理核心是MSK精密病理生物库中心的整体支柱，并提供了一个 利用独特的组织资源的基于人体组织的研究的综合资源 可在MSK上找到。对人类生物标本的获取和押金，用于研究原因， 癌症的检测，预防和治疗已成为基本和基础的必不可少的资源 MSK的转化癌研究人员。来自新的分子数据的可靠性 技术平台取决于获得足够的最佳采购高质量组织的供应 标本。 MSK的病理核心提供有效且具有成本效益的服务以促进组织使用 并进行人类基于生物测量的实验。核心也是国家资源 来自已提供给NCI资助的多种肿瘤类型的1000多种样品证明了 TCGA倡议以及将为NCI的CPTAC蛋白质组学贡献的样品 财团。 核心内的组织学服务有助于选择适当的标本进行组织分析和 提供了切割，解剖和染色的基本服务 嵌入组织。它包括构造组织微阵列块和激光捕获的微解剖 纯细胞群体和肿瘤细胞富集的分离。核心的免疫组织化学服务 使用优化的单克隆和多克隆抗体提供自动和手动染色 临床科学研究研究。该服务还不断开发并优化了新的协议 抗体和各种敏感检测系统。提供的服务和协作工作由 病理核心为10个中心中9名MSK研究人员的1,685个出版物做出了贡献 程序。这些论文中有许多发表在领先的癌症和一般兴趣期刊上。