Pathology

病理

• 批准号: 10571810
• 负责人: Michael H. A. Roehrl
• 金额: $ 76.36万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-20 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10571810
• 关键词: Antibodies; Basic Cancer Research; Biological; Blood specimen; Cancer Center Support Grant; Cells; Clinical; Collection; DNA; Data; Detection; Formalin; Freezing; Funding; Histologic; Histology; Human; Human Resources; Immunohistochemistry; Institution; Journals; Laboratories; Liquid substance; Malignant Neoplasms; Manuals; Molecular; Monoclonal Antibodies; Paper; Paraffin Embedding; Pathology; Population; Prevention; Procedures; Process; Proteins; Protocols documentation; Publications; Publishing; RNA; Research; Research Personnel; Resources; Sampling; Services; Source; Specimen; Stains; The Cancer Genome Atlas; Tissue Embedding; Tissue Microarray; Tissues; Work; anticancer research; biobank; cancer therapy; cost effective; design; detection platform; experimental study; human tissue; interest; laser capture microdissection; neoplastic; neoplastic cell; new technology; polyclonal antibody; programs; proteogenomics; research study; technology platform; tissue resource; translational cancer research; tumor

ABSTRACT The Pathology Core is an integral pillar of the MSK Precision Pathology Biobanking Center and provides a comprehensive resource for human tissue-based research that takes advantage of the unique tissue resources available at MSK. The acquisition and banking of human biologic specimens to be used to study causes, detection, prevention, and treatment of cancer have become an indispensible resource for basic and translational cancer researchers throughout MSK. The reliability of molecular data derived from new technology platforms depends on access to an adequate supply of optimally procured, high-quality tissue specimens. The Pathology Core at MSK provides efficient and cost-effective services to facilitate tissue use and to conduct human biospecimen-based experimentation. The Core also serves as a national resource, as evidenced by the over 1,000 samples from multiple tumor types that have been provided to the NCI-funded TCGA initiative, as well as the samples that will be contributed to the NCI’s CPTAC proteogenomics consortium. The Histology Service within the Core facilitates selection of appropriate specimens for tissue analyses and provides the basic services of cutting, dissecting, and staining of fresh-frozen and formalin-fixed, paraffin- embedded tissues. It includes construction of tissue microarray blocks and laser capture microdissection for isolation of pure cell populations and tumor cell enrichment. The Core’s Immunohistochemistry Service provides automated and manual staining using optimized monoclonal and polyclonal antibodies to be used in clinico-pathologic research studies. The service also continuously develops and optimizes protocols for new antibodies and for a variety of sensitive detection systems. The services and collaborative work provided by the Pathology Core have contributed to 1,685 publications of MSK researchers from nine of the 10 Center Programs. Many of these papers were published in leading cancer and general interest journals.

摘要 病理核心是MSK精密病理生物库中心不可或缺的支柱，并提供 利用独特的组织资源进行人体组织研究的综合资源 可在MSK上使用。获取和储存用于研究病因的人体生物标本， 癌症的检测、预防和治疗已成为基本和 MSK的转化型癌症研究人员。新数据来源的分子数据的可靠性 技术平台依赖于获得充足的最佳采购的高质量组织的供应 标本。MSK的病理学核心提供高效率和低成本的服务，以促进组织的使用 并进行以生物有机胺为基础的实验。核心也是一项国家资源，如 NCI资助的来自多种肿瘤类型的1000多个样本证明了这一点 TCGA倡议，以及将对NCI的CPTAC蛋白质组学做出贡献的样本 财团。 核心内的组织学服务促进选择适当的标本进行组织分析和 提供新鲜冷冻和福尔马林固定的石蜡切片、解剖和染色的基本服务. 嵌入的组织。它包括组织微阵列块的构建和激光捕获显微切割 纯细胞群体的分离和肿瘤细胞的浓缩。核心的免疫组织化学服务 使用优化的单抗和多克隆抗体提供自动和手动染色，以用于 临床病理研究。该服务还不断开发和优化新的协议 用于检测抗体和各种敏感的检测系统。提供的服务和协作工作 病理核心已经为10个中心中的9个发表了1,685篇MSK研究人员的论文 程序。这些论文中的许多都发表在领先的癌症和普通兴趣杂志上。