Pathology

病理

• 批准号: 10084822
• 负责人: Michael H. A. Roehrl
• 金额: $ 76.36万
• 依托单位: SLOAN-KETTERING INST CAN RESEARCH
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-01-20 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10084822
• 关键词: Antibodies; Biological; Blood specimen; Cancer Center Support Grant; Cells; Clinical; Collection; DNA; Data; Detection; Formalin; Freezing; Funding; Histologic; Histology; Human; Human Resources; Immunohistochemistry; Journals; Laboratories; Liquid substance; Malignant Neoplasms; Manuals; Molecular; Monoclonal Antibodies; Paper; Paraffin Embedding; Pathologic; Pathology; Population; Prevention; Procedures; Process; Proteins; Protocols documentation; Publications; Publishing; RNA; Research; Research Personnel; Resources; Sampling; Services; Source; Specimen; Stains; The Cancer Genome Atlas; Tissue Embedding; Tissue Microarray; Tissues; Work; anticancer research; base; biobank; cancer therapy; cost effective; design; detection platform; experimental study; human tissue; interest; laser capture microdissection; neoplastic; neoplastic cell; new technology; polyclonal antibody; programs; proteogenomics; research study; tissue resource; translational cancer research; tumor

ABSTRACT The Pathology Core is an integral pillar of the MSK Precision Pathology Biobanking Center and provides a comprehensive resource for human tissue-based research that takes advantage of the unique tissue resources available at MSK. The acquisition and banking of human biologic specimens to be used to study causes, detection, prevention, and treatment of cancer have become an indispensible resource for basic and translational cancer researchers throughout MSK. The reliability of molecular data derived from new technology platforms depends on access to an adequate supply of optimally procured, high-quality tissue specimens. The Pathology Core at MSK provides efficient and cost-effective services to facilitate tissue use and to conduct human biospecimen-based experimentation. The Core also serves as a national resource, as evidenced by the over 1,000 samples from multiple tumor types that have been provided to the NCI-funded TCGA initiative, as well as the samples that will be contributed to the NCI’s CPTAC proteogenomics consortium. The Histology Service within the Core facilitates selection of appropriate specimens for tissue analyses and provides the basic services of cutting, dissecting, and staining of fresh-frozen and formalin-fixed, paraffin- embedded tissues. It includes construction of tissue microarray blocks and laser capture microdissection for isolation of pure cell populations and tumor cell enrichment. The Core’s Immunohistochemistry Service provides automated and manual staining using optimized monoclonal and polyclonal antibodies to be used in clinico-pathologic research studies. The service also continuously develops and optimizes protocols for new antibodies and for a variety of sensitive detection systems. The services and collaborative work provided by the Pathology Core have contributed to 1,685 publications of MSK researchers from nine of the 10 Center Programs. Many of these papers were published in leading cancer and general interest journals.

摘要 病理学核心是MSK精密病理学生物库中心不可或缺的支柱， 利用独特的组织资源进行基于人体组织的研究的综合资源 可在MSK。获取和储存用于研究病因的人体生物标本， 癌症的检测、预防和治疗已成为基础和 转译癌症研究人员在整个MSK。新分子数据的可靠性 技术平台依赖于获得充足的最佳采购的高质量组织供应 标本MSK的病理学核心提供高效和具有成本效益的服务，以促进组织的使用 并进行基于人体生物素的实验。核心也是国家资源， 已经提供给国家癌症研究所资助的1,000多个肿瘤类型的样本证明了这一点。 TCGA倡议，以及将贡献给NCI的CPTAC蛋白质基因组学的样本 财团 核心组织学服务有助于选择合适的标本进行组织分析， 提供切割、解剖和染色新鲜冷冻和福尔马林固定的石蜡标本的基本服务， 包埋组织。它包括构建组织微阵列块和激光捕获显微切割， 分离纯细胞群和肿瘤细胞富集。The Core的免疫组化服务 提供使用优化的单克隆和多克隆抗体的自动和手动染色， 临床病理学研究。该服务还不断开发和优化协议， 抗体和各种灵敏的检测系统。提供的服务和协作工作， 病理学核心为来自10个中心中的9个的MSK研究人员的1,685篇出版物做出了贡献 程序.其中许多论文发表在领先的癌症和一般兴趣期刊上。