Core C: Comparative Pathology Core

核心 C：比较病理学核心

• 批准号: 10573310
• 负责人: Enrico Radaelli
• 金额: $ 27.89万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-02-15 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10573310
• 关键词: Address; Algorithms; Animal Experiments; Animal Model; Animals; Archives; Autopsy; Biological; Biological Markers; Biological Process; Board Certification; Canis familiaris; Carbon; Cells; Classification; Clinical; Collaborations; Collection; Comparative Pathology; Computer software; Consultations; Data; Dedications; Development; Ensure; Epithelium; Evaluation; Fibrosis; Goals; Growth; Hematoxylin and Eosin Staining Method; Histological Techniques; Histology; Histopathology; Image Analysis; Immune; Immunofluorescence Immunologic; Immunohistochemistry; In Situ Hybridization; Inflammation; Inflammatory; Infrastructure; Label; Laboratories; Lesion; Macrophage; Malignant - descriptor; Measurable; Modality; Modernization; Molecular; Morphology; Mus; Normal tissue morphology; Outcome; Paraffin Embedding; Pathologic; Pathologist; Pathology; Patients; Phenotype; Play; Population; Pre-Clinical Model; Preparation; Procedures; Protocols documentation; Protons; Radiation therapy; Reproducibility; Research Design; Research Personnel; Rodent; Role; Route; Sampling; Services; Severities; Signal Pathway; Sirius Red F3B; Slide; Solid; Specimen; Stains; Standardization; Structure; System; T-Lymphocyte; Techniques; Technology; Testing; Therapeutic Index; Time; Tissue Sample; Tissues; Toxic effect; Training; Translations; Trichrome stain method; Variant; Work; animal tissue; cytokine; digital imaging; digital pathology; efficacy evaluation; experience; functional status; immune cell infiltrate; immunological status; interest; neoplastic; neoplastic cell; neutrophil; particle; pathology imaging; personalized approach; pre-clinical; preclinical study; preservation; programs; response; tissue preparation; tool; translational study; tumor; tumor microenvironment; whole slide imaging

Project Summary The study of the preclinical animal models included in all four Projects plays a critical role to investigate the overall hypothesis that Proton/Carbon Particle FLASH Radiotherapy is superior to Standard Particle Radiotherapy in protecting normal tissues while maintaining equipotent malignant growth control. The extrapolation of robust preclinical data in these experimental settings relies heavily on the unbiased assessment of specific histopathological parameters to investigate the biological effects of FLASH particle radiotherapy as compared to Standard particle radiotherapy on designated neoplastic lesions and adjacent normal tissues. The Comparative Pathology Core (CPC; Core C) will provide its expertise contributing to the planning, evaluation, and interpretation of the pathology endpoints of the animal experiments included in the Projects. As a PennVet clinical laboratory, Core C importantly follows standardized working protocols with strict QA/QC procedures. The histopathology service offered by Core C will ensure accurate collection, proper preservation, timely processing, and staining of the animal specimens. Core C will provide the necessary technical support and submissions of tissue samples will follow a prioritized route. In addition to standard histopathology, Core C will also develop and validate tailored approaches to investigate and quantify tissue changes specifically associated with RT such as fibrosis, intralesional distribution of inflammatory/immune cell populations, expression of markers to evaluate epithelial barrier integrity, etc. The board-certified veterinary pathologists of Core C will deliver expert evaluation and unbiased interpretation of the pathology endpoints. Objective and reproducible quantification of tissue changes in response to the diverse RT modalities will be guaranteed by the application of validated scoring systems and the utilization of software-based algorithms for digital pathology and image analysis. Core pathologists have advanced training in digital imaging pathology from Leica pathology systems. Moreover, Dr. Assenmacher has collaborated with Leica in the development of analysis tools, lending him particular experience in the analytic modules of the digital pathology system.

项目摘要 所有四个项目中包括的临床前动物模型的研究对于研究 质子/碳粒子FLASH放射治疗上级标准粒子的总体假设 放射治疗在保护正常组织的同时维持等效的恶性生长控制。的 在这些实验环境中外推稳健的临床前数据在很大程度上依赖于无偏性 评估特定的组织病理学参数，以研究FLASH粒子的生物学效应 放射治疗与标准粒子放射治疗对指定肿瘤病变和邻近肿瘤病变的疗效比较 正常组织比较病理学核心（CPC;核心C）将提供其专业知识， 计划、评价和解释包括在 项目作为PennVet临床实验室，核心C重要的是遵循标准化的工作方案， QA/QC程序。核心C提供的组织病理学服务将确保准确收集、适当 动物标本的保存、及时处理和染色。核心C将提供必要的 技术支持和组织样本的提交将按照优先顺序进行。除了标准 组织病理学，核心C还将开发和验证定制的方法，以调查和量化组织 与RT特异性相关的变化，如纤维化、炎症/免疫细胞的病灶内分布 群体，标记物的表达以评估上皮屏障完整性等。 核心C的病理学家将提供专家评估和病理学终点的公正解释。 客观和可重复的量化组织变化的反应，以不同的RT模式将是 通过应用经过验证的评分系统和利用基于软件的算法来保证 用于数字病理学和图像分析核心病理学家在数字成像病理学方面接受过高级培训 徕卡病理系统。此外，Assenmacher博士还与徕卡合作开发了 分析工具，使他在数字病理学系统的分析模块方面拥有特别的经验。