Single cell molecular analysis of influenza vaccine responses in elderly individuals
Single cell molecular analysis of influenza vaccine responses in elderly individuals
批准号:
10574601
负责人:
Spyros A Kalams
金额:
$47.49万
依托单位国家:
美国
项目类别:
财政年份:
2019
资助国家:
美国
项目状态:
未结题
起止时间:
2019-03-01 至 2025-02-28
关键词:
AdjuvantAdultAgeAge YearsAgingAntibodiesAntibody titer measurementAntigensB-LymphocytesCD4 Positive T LymphocytesCell SeparationCellsCellular ImmunologyCytomegalovirusDoseElderlyFollow-Up StudiesFrequenciesFutureGenesHealthHelper-Inducer T-LymphocyteHospitalizationImmuneImmune responseImmunityImmunoglobulin-Secreting CellsImmunologic FactorsImpairmentIndividualInfluenzaInfluenza vaccinationLicensingMF59MolecularMolecular AnalysisPeripheralPlasma CellsPlasmablastPneumoniaPopulationPredictive FactorReportingSeasonsSeriesStructure of germinal center of lymph nodeT cell responseT-LymphocyteT-cell receptor repertoireTechniquesUnited StatesVaccinationVaccine Clinical TrialVaccinesalpha-beta T-Cell Receptorcohortdesignefficacy clinical trialhuman old age (65+)immune functionimprovedindividual responseinfluenza virus vaccinelymph nodespathogenpreventresponsesenescencesingle cell sequencingtranscriptomic profilingtranscriptomicstrial comparingvaccine responsevaccine trial
中文摘要
项目总结
英文摘要
PROJECT SUMMARY
We will use cutting edge single T cell analysis techniques to molecularly define the T cell repertoire and
function of cellular immune responses of individuals 65 years of age or older. The mechanisms behind
declining immune responses with age is likely multifactorial. Aging is associated with a reduction in the
frequency of antibody secreting cells and the number of lymph node germinal centers, and we hypothesize
this to be due to impaired ability of CD4+ T cells to provide adequate help to B cells. T follicular helper cells
in germinal centers directly provide help to B cells, and induce them to become antibody-secreting plasma
cells. We have previously demonstrated the frequencies of activated peripheral T follicular helper cells
(pTfh) and circulating plasmablasts shortly after vaccination is directly related to subsequent titers of
vaccine-specific antibodies. Furthermore, high dose influenza vaccine (IIV HD) was better able to induce
activated pTfh cells than standard dose vaccine (IIV SD). In this application we will probe the mechanisms
behind successful immune responses to vaccines in an elderly population. We will evaluate the immune T
cell repertoire, the transcriptomic profile of responding cells, and inflation of CMV-reactive T cells in elderly
individuals and determine the effect of these factors on the host's ability to mount successful immune
responses to vaccines.
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Single cell molecular analysis of influenza vaccine responses in elderly individuals
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批准号:10887735
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项目类别:
-
资助金额:$43.65万
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财政年份:2019
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负责人:Spyros A Kalams
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依托单位:
Single cell molecular analysis of influenza vaccine responses in elderly individuals
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批准号:10383653
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项目类别:
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资助金额:$47.39万
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财政年份:2019
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负责人:Spyros A Kalams
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依托单位:
Molecular analysis of the adaptive immune response to tuberculosis
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批准号:9204642
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项目类别:
-
资助金额:$23.41万
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财政年份:2016
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负责人:Spyros A Kalams
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依托单位:
Molecular analysis of the adaptive immune response to tuberculosis
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批准号:9346014
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项目类别:
-
资助金额:$19.75万
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财政年份:2016
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负责人:Spyros A Kalams
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依托单位:
Laboratory Sciences Core (Core D)
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批准号:10404938
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项目类别:
-
资助金额:$33.29万
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财政年份:2015
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负责人:Spyros A Kalams
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依托单位:
Laboratory Sciences Core (Core D)
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批准号:10617300
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项目类别:
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资助金额:$23.46万
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财政年份:2015
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负责人:Spyros A Kalams
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依托单位:
Laboratory Sciences Core (Core D)
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批准号:10153673
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项目类别:
-
资助金额:$50.99万
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财政年份:2015
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负责人:Spyros A Kalams
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依托单位:
Pro-Apoptotic BCG as an HCV vaccine vector
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批准号:7386674
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项目类别:
-
资助金额:$24.56万
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财政年份:2009
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负责人:Spyros A Kalams
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依托单位:
Pro-Apoptotic BCG as an HCV vaccine vector
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批准号:7936880
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项目类别:
-
资助金额:$19.33万
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财政年份:2009
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负责人:Spyros A Kalams
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依托单位:
Characterization of T & B cell responses correlated with broad neutralizing Abs
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批准号:7663383
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项目类别:
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资助金额:$53.16万
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财政年份:2009
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负责人:Spyros A Kalams
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依托单位:
Characterization of T & B cell responses correlated with broad neutralizing Abs
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批准号:8913416
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项目类别:
-
资助金额:$36.95万
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财政年份:2009
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负责人:Spyros A Kalams
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依托单位:
Molecular Analysis of HCV-specific T Cell Responses
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批准号:7014196
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项目类别:
-
资助金额:$23.93万
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财政年份:2005
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负责人:Spyros A Kalams
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依托单位:
Vanderbilt Meharry Center for AIDS Research
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批准号:8304644
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项目类别:
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资助金额:$64.47万
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财政年份:2003
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负责人:Spyros A Kalams
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依托单位:
HIV IMMUNOPATHOGENESIS
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批准号:8330528
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项目类别:
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资助金额:$14.23万
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财政年份:2003
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负责人:Spyros A Kalams
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依托单位:
Molecular analysis of HCV T cell responses
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批准号:6652717
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项目类别:
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资助金额:$43.42万
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财政年份:2002
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负责人:Spyros A Kalams
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依托单位:
Molecular analysis of HCV T cell responses
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批准号:6493586
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项目类别:
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资助金额:$43.42万
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财政年份:2001
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负责人:Spyros A Kalams
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依托单位:
Molecular analysis of HCV T cell responses
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批准号:6349586
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项目类别:
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资助金额:$43.42万
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财政年份:2000
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负责人:Spyros A Kalams
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依托单位:
TCR Genes and HIV-Specific Cytotoxic T-lymphocytes
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批准号:6496461
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项目类别:
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资助金额:$33.79万
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财政年份:1997
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负责人:Spyros A Kalams
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依托单位:
TCR GENE USAGE AND QUANTITATION OF HIV SPECIFIC CTL
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批准号:2672781
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项目类别:
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资助金额:$20.03万
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财政年份:1997
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负责人:Spyros A Kalams
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依托单位:
TCR GENE USAGE AND QUANTITATION OF HIV SPECIFIC CTL
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批准号:2330455
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项目类别:
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资助金额:$20.9万
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财政年份:1997
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负责人:Spyros A Kalams
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依托单位:
海外基金