Phenotyping Core

表型核心

• 批准号: 10579074
• 负责人: GERALD I SHULMAN
• 金额: $ 60.48万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10579074
• 关键词: Collaborations; Communities; Databases; Development; Diabetes Mellitus; Diabetic mouse; Extramural Activities; Functional disorder; Isotopes; Mass Fragmentography; Metabolic; Metabolism; Methodology; Methods; Modeling; Mus; Obesity; Phenotype; Price; Radiolabeled; Research Personnel; Services; Standardization; Tissues; awake; cost; empowerment; genetic manipulation; in vivo; insight; metabolic phenotype; mouse model; novel; novel therapeutics; phenotypic data; precision medicine; stable isotope

The Overarching Aim of the Yale MPMOD Phenotyping Core is to provide extramural investigators access to the unique metabolic phenotyping services provided by the Yale MPMOD at a fair and reasonable price, and empower them to characterize the impact of their genetically manipulated mouse models of diabetes and obesity on key metabolic parameters and whole body and tissue specific intracellular metabolic fluxes using state-of- the-art stable and radio-labeled isotope methodology. The Specific Aims of the Yale MPMOD Phenotyping Core are: 1) To provide the scientific community access to standardized, high quality, unique metabolic phenotyping services currently available at the Yale MPMOD at a fair and reasonable cost, 2) To continue to develop novel stable isotope GC-MS, LC-MS/MS and NMR methods to metabolically phenotype whole body and intracellular metabolism in awake mice, and 3) To participate and collaborate with the National Consortium of MPMODs and the MPMOD Coordinating Unit to maintain a national database of metabolic phenotyping data in mouse models of obesity and diabetes. It is anticipated that the results generated by the Yale MPMOD will lead to a better understanding of the pathophysiology of diabetes and obesity and their related metabolic complications and lead to new therapies. Ultimately, the insights generated from in vivo metabolic phenotyping in mouse models will facilitate the development of precision medicine approaches targeting metabolic pathophysiology and its downstream complications.

耶鲁大学MPMOD表型核心的总体目标是为校外研究人员提供访问权限 耶鲁大学MPMOD以公平合理的价格提供的独特代谢表型分析服务，以及 使他们能够描述他们的基因操纵的糖尿病和肥胖症小鼠模型的影响， 关键代谢参数以及全身和组织特异性细胞内代谢通量， 最先进的稳定和放射性标记的同位素方法。Yale MPMOD表型分析的具体目的 核心是：1）为科学界提供标准化、高质量、独特的代谢 表型分析服务目前可在耶鲁大学MPMOD在一个公平和合理的成本，2）继续 开发新的稳定同位素GC-MS、LC-MS/MS和NMR方法，以代谢表型整个身体， 清醒小鼠的细胞内代谢，和3）参与并与国家联盟合作， MPMOD和MPMOD协调单位，以维护国家代谢表型数据库， 肥胖和糖尿病的小鼠模型。预计耶鲁大学MPMOD产生的结果将导致 更好地了解糖尿病和肥胖的病理生理学及其相关的代谢 并发症并导致新的治疗方法。最终，从体内代谢表型分析中产生的见解 在小鼠模型中的应用将促进针对代谢的精确医学方法的发展 病理生理学及其下游并发症。