Yale Center for Metabolic Phenotyping in Live Models of Obesity and Diabetes

耶鲁大学肥胖和糖尿病活体模型代谢表型中心

• 批准号: 10579071
• 负责人: GERALD I SHULMAN
• 金额: $ 79.56万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10579071
• 关键词: Animals; Area; Biomedical Research; Client; Collaborations; Communities; Complex; Data Analyses; Data Storage and Retrieval; Diabetes Mellitus; Experimental Designs; Extramural Activities; Funding; Goals; Guidelines; Internships; Learning; Liver; Metabolic; Metabolic Diseases; Metadata; Methodology; Methods; Modeling; Monitor; Mus; Obesity; Phenotype; Physiological; Protocols documentation; Quality Control; Reproducibility; Research; Research Personnel; Resources; Services; Standardization; Technology; Testing; Training; Underrepresented Minority; Work; behavioral phenotyping; complex data; cost; data format; data standards; digital object identifier; diversity and equity; empowerment; health equity promotion; metabolic phenotype; minority investigator; mouse genetics; mouse model; operation; programs; screening; testing services; web-based tool

The overarching goal of the Yale MPMOD is to provide extramural investigators access to the unique metabolic phenotyping services provided by the Yale MPMOD and empower them to harness the power of mouse genetics to its fullest potential to metabolically phenotype their mouse models of obesity and diabetes. The Yale MPMOD consists of three highly interactive Cores that have an established (>20 yr) track record of working together in close harmony: 1) The Yale MPMOD Administrative Core oversees the operation of the Yale MPMOD, facilitates research with the other MPMOD Centers and the MPMOD Coordinating Unit to standardize key methodologies, and coordinates the efficient workflow through the Yale MPMOD cores and access to the Yale MPMOD Animal Core and the Yale MPMOD Phenotyping Core, 2) The Yale MPMOD Animal Core provides a centralized facility for coordinating receiving, screening and monitoring mice from outside investigators and 3) The Yale MPMOD Phenotyping Core empowers Yale MPMOD clients with access to unique metabolic phenotyping services that provides them with the means to characterize the metabolic changes in their particular mouse models of complex metabolic disease using established state-of-the-art methodology. The Specific Aims of the Yale MPMOD are to: 1) serve the US academic research community to study diabetes and obesity in their mouse models by providing metabolic, physiologic, and behavioral phenotyping on live mice, as well as expert advice in mouse models, experiment design, data analysis, and data interpretation at similar cost whether inside or outside Yale without bias and, and collaborate to provide a wide array of tests and expert advice while avoiding unnecessary overlap, 2) Establish a Yale MPMOD Vibrant Program, which will work with the other national MPMODs and the MPMOD Coordinating Unit to promote health equity and diversity in the US biomedical research enterprise by supporting underrepresented minority investigators to succeed in biomedical research in the areas of diabetes and obesity, via resources such as test services, pilot funding, expert advice on experimental design, and/or short internships at the Yale MPMOD to learn test technologies, 3) Promote rigor and reproducibility in research by: a) developing and sharing validated protocols for phenotyping live mice; b) providing difficult experimental tests conducted by experts with a high degree of standardization and quality control, to PIs who would otherwise not be able to conduct or afford them; and c) sharing technologies and providing web-based tools for gold-standard approaches in experimental methods and data analysis, 4). Develop standardized data formats and storage guidelines for complex data, including metadata, for sharing with clients and the public as appropriate, and employ unique Research Resource identifiers (RRID), Digital Object Identifiers (DOI), and other best practices directed by DKNet.

耶鲁大学mpmod的总体目标是提供壁外调查人员使用独特的代谢 耶鲁MPMOD提供的表型服务，并授权他们利用鼠标的力量 它具有最大的潜力，可以使它们的肥胖和糖尿病小鼠模型为代谢表型。耶鲁的mpmod 由三个高度互动的核心组成 紧密和谐：1）耶鲁MPMOD行政核心负责监督耶鲁MPMOD的操作， 促进与其他MPMOD中心和MPMOD协调单元的研究，以标准化密钥 方法论，并通过耶鲁mpmod核心协调有效的工作流程并访问耶鲁 mpmod动物核心和耶鲁MPMOD表型核心，2）耶鲁MPMOD动物核心提供 用于协调接收，筛查和监测小鼠的集中设施，3） 耶鲁大学MPMOD表型核心使耶鲁大学MPMOD客户能够访问独特的代谢表型 服务为他们提供表征其特定鼠标模型中代谢变化的手段 使用已建立的最先进方法论的复杂代谢疾病。耶鲁大学的具体目标 mpmod是：1）为美国的学术研究界服务鼠标中的糖尿病和肥胖症 通过为活小鼠提供代谢，生理和行为表型的模型以及专家建议 在鼠标模型中，实验设计，数据分析和数据解释类似成本，无论是内部还是 耶鲁大学外面没有偏见，并协作提供各种各样的测试和专家建议，同时避免 不必要的重叠，2）建立耶鲁大学MPMOD充满活力的计划，该计划将与另一个国家合作 mpmods和mpmod协调单位，以促进美国生物医学的健康公平和多样性 研究企业通过支持代表性不足的少数群体研究人员在生物医学研究中取得成功 糖尿病和肥胖领域通过测试服务，试点资金，专家建议等资源 耶鲁大学学习测试技术的实验设计和/或短暂的实习，3）促进严格 以及研究中的可重复性：a）制定和共享经过验证的现场小鼠方案； b） 提供高标准化和质量的专家进行的困难实验测试 控制原本无法进行或负担得起的PI； c）共享技术和 在实验方法和数据分析中提供基于网络标准的方法的工具，4）。发展 包括元数据在内的复杂数据的标准化数据格式和存储指南，用于与客户共享 公众适当，并采用独特的研究资源标识符（RRID），数字对象 标识符（DOI）以及DKNET指导的其他最佳实践。