Mapping gustatory neuron types.

映射味觉神经元类型。

• 批准号: 10578690
• 负责人: Nirupa Chaudhari
• 金额: $ 39.72万
• 依托单位: UNIVERSITY OF MIAMI SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10578690
• 关键词: Address; Afferent Neurons; Anatomy; Antibodies; Bioinformatics; Brain; Brain Stem; Citric Acid; Code; Communication; Data; Dedications; Dextrans; Duct (organ) structure; Ear; FOS gene; Foliate Papilla; Fungiform Papilla; Genes; Genetic Markers; Genetic Recombination; Heterogeneity; Hybrids; Image; Immunofluorescence Immunologic; Incisor; Individual; Label; Maps; Modality; Molecular; Mouse Strains; Mus; Nerve; Neural Pathways; Neuroanatomy; Neurons; Nucleus solitarius; Oral; Pathway interactions; Peripheral; Population; Process; Property; Receptor Cell; Reporting; Research; Resolution; Role; Sensory; Sensory Ganglia; Signal Transduction; Sodium Glutamate; Soft Palate; Spinal Ganglia; Stimulus; Structure; Structure of geniculate ganglion; Sucrose; Tactile; Taste Bud Cell; Taste Buds; Taste Perception; Techniques; Testing; Texture; Transgenic Mice; Visualization; biomarker validation; cell type; detection of nutrient; dorsal horn; experimental study; in vivo; insight; molecular marker; neuronal circuitry; neurotransmission; novel; receptive field; response; segregation; single-cell RNA sequencing; sugar; transcriptome; transmission process

PROJECT SUMMARY The afferent sensory neurons that innervate taste buds have eluded definition at the molecular level until very recently. Specifically, it has been unclear if separate classes of neurons innervate the well-established receptor cell types in the taste bud. It has also been unclear whether there are dedicated neuronal circuits that connect peripheral receptor cells, afferent sensory neurons and the first relay neurons in the Nucleus of the Solitary Tract in the brainstem. Progress has been impeded by the lack of molecular markers to distinguish gustatory neuronal cell types. We have recently completed a study on single-cell RNAseq of geniculate ganglion neurons. Our detailed bioinformatics analyses, which are publicly accessible, revealed neuronal subtypes based on deep separations in their transcriptome profiles. Our study also yielded markers which permit us to distinguish the classes of gustatory neurons. We propose to examine the three main classes, T1, T2 and T3, in several ways. We will assess whether each class of neurons associates with taste buds in a given receptive field, or with particular taste bud cells types. We will also examine the central projections of neurons of each class for insights into their potential roles in gustation. Second, we will evaluate whether the gustatory neuron types preferentially convey information on particular taste qualities. And finally, we will examine the significance of the novel hybrid gustatory-mechanosensory neuron type that we recently reported, using in vivo confocal Ca2+ imaging in transgenic mice expressing the Ca2+ indicator, GCaMP. Together, these analyses will be the first to assess the extent of heterogeneity in the peripheral and central connectivity and functional properties of defined subtypes of gustatory neurons.

项目摘要 支配味蕾的传入感觉神经元在分子水平上没有明确的定义。 水平，直到最近。具体来说，目前还不清楚不同类别的神经元是否 使味蕾中的成熟受体细胞类型受神经支配。也不清楚 是否有专门的神经元回路连接外周受体细胞， 感觉神经元和脑干孤束核中的第一中继神经元。 由于缺乏区分味觉神经元的分子标记物， 细胞类型。我们最近完成了膝状神经节单细胞RNAseq的研究， 神经元我们详细的生物信息学分析，这是公开的，揭示了神经元 亚型基于其转录组谱中的深度分离。我们的研究还发现， 这些标记使我们能够区分味觉神经元的类别。我们建议 从几个方面考察三个主要的类，T1，T2和T3。我们将评估每一个 一类神经元与特定感受野中的味蕾或特定味蕾相关 细胞类型。我们还将检查每类神经元的中央投射，以获得见解 它们在味觉中的潜在作用其次，我们将评估味觉神经元是否 类型优先传达关于特定味道品质的信息。最后，我们将研究 新的混合味觉-机械感觉神经元类型的意义，我们最近， 报道，在表达Ca 2+指示剂的转基因小鼠中使用体内共聚焦Ca 2+成像， GCaMP。总之，这些分析将是第一次评估异质性的程度， 味觉神经元亚型的外周和中枢连接及功能特性 神经元