Acquisition of a Crystal Gryphon-LCP Crystallization Robot

采购 Crystal Gryphon-LCP 结晶机器人

• 批准号: 10582203
• 负责人: MATTHIEU GAGNON
• 金额: $ 10.61万
• 依托单位: UNIVERSITY OF TEXAS MED BR GALVESTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-18 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10582203
• 关键词: Administrative Supplement; Communities; Complex; Crystallization; Drops; Funding; Maintenance; National Institute of General Medical Sciences; Protein Biosynthesis; Recycling; Reporting; Ribosomes; Robot; Sampling; Sea; Speed; Structure; System; Techniques; Training; X-Ray Crystallography; base; cost; equipment acquisition; inhibitor; instrument; operation; parent grant; public health relevance; response; screening; structural biology; translation factor

ABSTRACT In response to NOT-GM-22-017, Notice of Availability of Administrative Supplements for Equipment Purchases for NIGMS Awardees, we request the funds to purchase the Crystal Gryphon-LCP Crystallization Robot. Our parent grant aims to establish the structural bases regulating the key steps of protein synthesis: initiation, termination, and ribosome recycling. While several crystal structures of the ribosome in complex with inhibitors have been reported, the mechanisms that underline inhibition of translation factors off the ribosome remain poorly understood. To enable structure determination and speed up as well as decrease the cost of crystallizing our targets, we need a high-throughput crystallization system that can efficiently identify successful crystallization conditions. The requested system offers sufficient high throughput to support not only the Gagnon lab, but also the other x-ray crystallography labs within the Sealy Center for Structural Biology (SCSB), requires minimal sample material, reliably uses either commercial crystallization screening kits (96-well SBS format) or specially created screens, performs optimization using 96-well, 24-well, sitting-drop, or hanging-drop techniques, and adds the capability of microseeding, which is proving essential to our project. In recognition of the broad benefit of this instrument to the structural biology community within UTMB, the SCSB is committing space, long-term maintenance, and staff for operation and user training of the instrument.

摘要 针对NOT-GM-22-017《关于提供设备采购行政补充的通知》 对于NIGMS获奖者，我们请求资金购买Crystal Gryphon-LCP结晶机器人。我们的 Parent Grate旨在建立调控蛋白质合成关键步骤的结构基础：启动， 终止和核糖体回收。而核糖体的几种晶体结构与抑制剂形成了络合物 已经报道过，强调抑制核糖体外翻译因子的机制仍然存在 人们对此知之甚少。能够确定结构，加快结晶速度，降低结晶成本 我们的目标，我们需要一个高通量的结晶系统，可以有效地识别成功的结晶 条件。所请求的系统提供了足够的高吞吐量，不仅可以支持Gagnon实验室，还可以 Sealy结构生物学中心(SCSB)内的其他X射线结晶学实验室需要最低限度的 样品材料，可靠地使用商用结晶筛选试剂盒(96孔SBS格式)或专门 创建屏幕，使用96井、24井、坐放或挂放技术执行优化，以及 增加了微播种的能力，这对我们的项目是必不可少的。认识到广泛的利益 对于UTMB内的结构生物学社区，SCSB正在致力于长期的空间 维护以及仪器操作和用户培训的工作人员。