Calcium coding mechanisms in plant cell growth and immunity

植物细胞生长和免疫中的钙编码机制

• 批准号: 10581257
• 负责人: Sheng Luan
• 金额: $ 6.67万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10581257
• 关键词: Animals; Arabidopsis; Calcium; Calcium Signaling; Code; Containment; Controlled Environment; DNA; Ensure; Equipment; Eukaryota; Floods; Floor; Foundations; Funding; Future; Genetically Modified Organisms; Growth; Health; Human; Immune response; Immunity; Maintenance; Physiological Processes; Plant Model; Plants; Preventive; Second Messenger Systems; Solid; Specific qualifier value; Transgenic Organisms; Universities; cell growth; genetic approach; human old age (65+); neuron development; new growth; parent project; pathogen; pathogenic bacteria; plant growth/development; programs

This equipment supplement application seeks funding for replacement of a broken growth chamber for culturing plant materials used in the parent project. The model plant Arabidopsis must be grown and maintained in a controlled environments. In particular, our plants are routinely transgenic containing foreign DNAs and considered “genetically modified organisms” (GMOs) and thus must be contained in a specified chamber. We are also working with pathogenic bacteria that induce immune responses in plants. Such pathogens also require containment. We have been using the Conviron plant growth chambers for these critical purposes. We had two old growth chambers (27 years) at the beginning of the parent project. An accidental floor flooding and leak above the growth chamber facility occurred and one of the growth chambers was severely damaged and we requested funding through the same supplement program in 2021 to replace it. In February 2022, the other old chamber failed due to old age. Two growth chambers are minimally required to grow plants with and without pathogen treatments. I thus request funding herein to purchase a new chamber to replace the failed chamber to ensure steady progress of the project and reduce the chance of cross-contamination of plant materials. To ensure the growth chamber operates correctly and are maintained properly, we propose to purchase the growth chamber from the same company Conviron so that our lab is familiar with the use of the chamber. Our University greenhouse and facility staff team will commit to the preventive maintenance of the growth chamber. We expect to have the new chamber in use as early as August 2022 should the funding become available in July. The two new growth chambers will provide a solid foundation for supplying plant materials in this funding period and future renewal of the project.

该设备补充申请寻求资金以更换损坏的生长物 用于培养母项目中使用的植物材料的室。模式植物拟南芥 必须在受控环境中生长和维护。特别是，我们的工厂是 通常含有外来 DNA 的转基因并被视为“转基因” 有机体”（转基因生物），因此必须包含在指定的室内。我们也在努力 与诱导植物免疫反应的病原菌。此类病原体还 需要遏制。我们一直在使用 Conviron 植物生长室来实现这些目的 关键目的。我们在母公司成立之初有两个旧的生长室（27年） 项目。生长室设施上方发生意外的地板洪水和泄漏， 其中一个生长室遭到严重损坏，我们请求通过 2021 年相同的补充计划将取代它。 2022年2月，另一间旧室 因年老而失败。种植植物至少需要两个生长室 未经病原体治疗。因此，我在此请求资金购买一个新的房间 更换故障室，确保项目稳步推进，减少事故发生的机会 植物材料的交叉污染。 为了确保生长室正确运行并正确维护，我们 建议从同一家公司Conviron购买生长室，以便我们的实验室 熟悉腔室的使用。我们的大学温室和设施工作人员团队将 致力于生长室的预防性维护。我们期望有新的 如果 7 月份资金到位，该室最早将于 2022 年 8 月投入使用。这 两个新的生长室将为该地区植物材料的供应奠定坚实的基础 资助期限和项目的未来更新。