Calcium coding mechanisms in plant cell growth and immunity

这项设备补充申请寻求资金，以取代一个破碎的增长 用于培养母公司项目中使用的植物材料的试验室。模式植物拟南芥 必须在受控环境中生长和维护。特别是，我们的植物是 常规的含有外来DNA的转基因，被认为是“转基因的” 生物体“(GMO)，因此必须放在指定的容器中。我们也在努力 在植物中诱导免疫反应的致病细菌。这样的病原体也 需要遏制。我们一直在使用康维龙植物生长室来处理这些 关键目的。在父母出生之初，我们有两个旧的生长室(27年) 项目。生长室设施上方发生了意外的地板淹水和泄漏， 其中一个生长室严重受损，我们通过 同样的补充计划将在2021年取代它。2022年2月，另一个旧会议厅 由于年事已高而失败。最低限度需要两个生长室来种植和 没有病原体治疗。因此，我在此请求提供资金，以购买一个新的会议厅 更换出现故障的会议厅，确保项目稳步推进，减少机会 植物材料的交叉污染。 为了确保生长室正常运行和正常维护，我们 建议从同一家公司Convion购买增长室，以便我们的实验室 熟悉暗室的使用方法。我们的大学温室和设施工作人员团队将 承诺对生长室进行预防性维护。我们期待着有新的 如果资金在7月到位，商会最早在2022年8月投入使用。这个 两个新的生长室将为供应植物材料提供坚实的基础 项目的资金期和未来续期。

This equipment supplement application seeks funding for replacement of a broken growth chamber for culturing plant materials used in the parent project. The model plant Arabidopsis must be grown and maintained in a controlled environments. In particular, our plants are routinely transgenic containing foreign DNAs and considered “genetically modified organisms” (GMOs) and thus must be contained in a specified chamber. We are also working with pathogenic bacteria that induce immune responses in plants. Such pathogens also require containment. We have been using the Conviron plant growth chambers for these critical purposes. We had two old growth chambers (27 years) at the beginning of the parent project. An accidental floor flooding and leak above the growth chamber facility occurred and one of the growth chambers was severely damaged and we requested funding through the same supplement program in 2021 to replace it. In February 2022, the other old chamber failed due to old age. Two growth chambers are minimally required to grow plants with and without pathogen treatments. I thus request funding herein to purchase a new chamber to replace the failed chamber to ensure steady progress of the project and reduce the chance of cross-contamination of plant materials. To ensure the growth chamber operates correctly and are maintained properly, we propose to purchase the growth chamber from the same company Conviron so that our lab is familiar with the use of the chamber. Our University greenhouse and facility staff team will commit to the preventive maintenance of the growth chamber. We expect to have the new chamber in use as early as August 2022 should the funding become available in July. The two new growth chambers will provide a solid foundation for supplying plant materials in this funding period and future renewal of the project.