Calcium coding mechanisms in plant cell growth and immunity

这一设备补充申请寻求资金，以更换破碎的增长 用于培养母项目中使用的植物材料的室。模式植物拟南芥 必须在受控的环境中生长和维持。特别是，我们的工厂 常规转基因含有外来DNA，并被认为是“转基因的 因此，必须将其包含在指定的腔室中。我们还在努力 病原菌在植物中诱导免疫反应。这些病原体还 需要控制我们一直在使用Conviron植物生长箱， 关键目的。我们有两个旧的生长室（27年）在开始的父 项目生长室设施上方发生意外地板浸水和泄漏， 其中一个生长室严重受损，我们通过 2022年2月，另一个旧腔室将在2021年更换。 因年老而失败。最低限度需要两个生长室来种植植物， 没有病原体治疗。因此，我在此请求提供资金，购买一个新的会议厅， 更换损坏的闸室，以确保工程稳步进行， 植物材料的交叉污染。 为确保培养箱正确运行和维护，我们 我建议从同一家公司Conviron购买生长箱，以便我们的实验室 他熟悉这个房间的使用。我们的大学温室和设施工作人员团队将 承诺对生长室进行预防性维护。我们希望有新的 如果资金在7月到位，最早将于2022年8月投入使用。的 两个新的生长室将提供一个坚实的基础，为供应植物材料， 项目的融资期限和未来的延期。

This equipment supplement application seeks funding for replacement of a broken growth chamber for culturing plant materials used in the parent project. The model plant Arabidopsis must be grown and maintained in a controlled environments. In particular, our plants are routinely transgenic containing foreign DNAs and considered “genetically modified organisms” (GMOs) and thus must be contained in a specified chamber. We are also working with pathogenic bacteria that induce immune responses in plants. Such pathogens also require containment. We have been using the Conviron plant growth chambers for these critical purposes. We had two old growth chambers (27 years) at the beginning of the parent project. An accidental floor flooding and leak above the growth chamber facility occurred and one of the growth chambers was severely damaged and we requested funding through the same supplement program in 2021 to replace it. In February 2022, the other old chamber failed due to old age. Two growth chambers are minimally required to grow plants with and without pathogen treatments. I thus request funding herein to purchase a new chamber to replace the failed chamber to ensure steady progress of the project and reduce the chance of cross-contamination of plant materials. To ensure the growth chamber operates correctly and are maintained properly, we propose to purchase the growth chamber from the same company Conviron so that our lab is familiar with the use of the chamber. Our University greenhouse and facility staff team will commit to the preventive maintenance of the growth chamber. We expect to have the new chamber in use as early as August 2022 should the funding become available in July. The two new growth chambers will provide a solid foundation for supplying plant materials in this funding period and future renewal of the project.