Genomics, Bioinformatics & Biostatistics, and Microphysiological Systems Core

基因组学、生物信息学和生物统计学以及微生理系统因素 核心（GBBM-FC）摘要 众所周知，化学暴露于生物系统导致许多细胞因子的表达变化。 RNA和蛋白质分子，这些变化与毒性相关，并可指示毒性。在 此外，许多分子流行病学研究已经确定了遗传多态性之间的相关性， 和环境相关疾病的发病率。毒理学监测越来越多地涉及 评估来自人类个体和动物模型的遗传和其他分子测量， 检测疾病易感性的标志物，并确定化学效应的早期指标。各种有针对性 分子方法以及基因组学、表观基因组学、转录组学、蛋白质组学和代谢组学 近年来已经开发了一些方法，并且这些方法继续快速发展。这些方法 相互补充，并允许整个生物途径和网络的机制研究， 以及它们各自的组成部分。这些最先进的方法的最佳应用需要 在a）样品制备和处理，B）样品的生成和质量评估， 数据，c）严格的统计和生物信息学分析，d）以及复杂数据的解释。最 调查人员没有财力或专门知识来跟上快速发展的趋势， 这些领域的技术进步。然而，调查人员必须能够获得最新的 技术，以便具有竞争力并进行尖端研究。核心解决这些问题 通过提供获得基于OMIC的方法的必要专业知识， 环境卫生科学研究。核心还提供了先进的体外系统， 模拟复杂的组织结构，并提供平台，以研究器官样生理学的背景下， 毒理学扰动。核心的主要目标是使中心附属机构能够整合 最先进的基因组学、转录组学、表观基因组学、蛋白质组学和代谢组学技术， 以及微生理体外系统到他们的环境健康科学研究， 成本效益的方式。

GENOMICS, BIOINFORMATICS & BIOSTATISTICS, AND MICROPHYSIOLOGICAL SYSTEMS FACILITY CORE (GBBM-FC) ABSTRACT It is well established that chemical exposures to biological systems result in expression changes of numerous RNA and protein molecules and these changes are correlated with, and can be indicative of toxicity. In addition, many molecular epidemiologic studies have identified correlations between genetic polymorphisms and the incidence of environmentally-related diseases. Toxicological monitoring increasingly involves the assessment of genetic and other molecular measurements derived from human individuals and animal models, to detect markers of disease susceptibility and to identify early indicators of chemical effect. Various targeted molecular methods as well as genomics, epigenomics, transcriptomics, proteomics and metabolomics approaches have been developed in recent years and continue to develop rapidly. These methods complement each other and allow for mechanistic investigations of entire biological pathways and networks, as well as their individual components. The optimal application of these state-of-the-art methodologies requires considerable expertise in a) sample preparation and processing, b) generation and quality assessment of the data, c) rigorous statistical and bioinformatics analysis, d) as well as interpretation of the complex data. Most investigators do not possess the financial resources or specialized expertise to keep up with the rapid technological advancements in these areas. However, it is critical for investigators to have access to the latest technologies in order to be competitive and to perform cutting-edge research. The Core addresses these challenges by providing access to the necessary expertise in OMICs-based methods that support environmental health sciences research. The Core also provides access to sophisticated in vitro systems that mimic complex tissue architecture and provide platforms to investigate organ-like physiology in the context of toxicological perturbations. The primary goal of the Core is to empower Center affiliates to integrate state-of-the-art genomics, transcriptomics, epigenomics, proteomics, and metabolomics technologies, as well as microphysiological in vitro systems into their Environmental Health Sciences research in a cost-effective manner.