Mechanisms of vaccine immunity against coccidioidomycosis

球孢子菌病疫苗免疫机制

• 批准号: 10584260
• 负责人: BRUCE Steven KLEIN
• 金额: $ 53.58万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-02-01 至 2028-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10584260
• 关键词: Ablation; Address; Antigen-Presenting Cells; Antigens; Attenuated Vaccines; Black Populations; Blastomyces dermatitidis; CD4 Positive T Lymphocytes; CYP21A2 gene; Calcineurin; Canis familiaris; Cell Shape; Cells; Cellular Immunity; Coccidioides; Coccidioidomycosis; Data; Disease; Early Mobilizations; Elements; Emerging Communicable Diseases; Epithelial Cells; Epithelial Receptor Cell; Epithelium; Filipino; Genes; Genetic Polymorphism; Hispanic Populations; Human; Immunity; Immunodominant Antigens; Infection; Inflammatory; Inflammatory Response; Inhalation; Kinetics; Knowledge; Label; Link; Lung; Lymphocyte; Lymphoid Tissue; M cell; Measures; Mediating; Memory; Methods; Microbe; Military Personnel; Morbidity - disease rate; Mucosal Immunity; Mucous Membrane; Mus; Mycoses; Myelogenous; Myeloid Cells; NF-kappa B; Natural Immunity; PLCG2 gene; PPP3R1 gene; Patients; Peptides; Peyer' s Patches; Phenotype; Pregnant Women; Prevention; Process; Reagent; Reporting; Reproduction spores; Research; Resistance; Respiratory Mucosa; Role; Route; Signal Pathway; Signal Transduction; Structure of parenchyma of lung; Subunit Vaccines; Surface; T-Cell Development; T-Lymphocyte; Testing; Tissues; Training; Transgenic Mice; Translating; United States National Institutes of Health; Vaccination; Vaccines; Work; adaptive immunity; commercialization; cytokine; dectin 1; draining lymph node; fungus; infection rate; innovation; memory CD4 T lymphocyte; mortality; mucosal vaccine; next generation; novel; pathogen; priority pathogen; receptor; recruit; response; tool; trafficking; transcriptome; uptake; vaccine delivery; vaccine trial

ABSTRACT Coccidioidomycosis is a re-emerging infection that NIH has prioritized for vaccine prevention. An experimental vaccine of live attenuated spores is highly protective after intranasal delivery. We propose to study this vaccine to define mechanisms by which lung epithelium regulates durable mucosal T cell immunity. Resistance against inhaled microbes is thought to reside within tissue-resident memory (TRM) cells, but little is known about how this intranasal vaccine induces lung TRM. Our preliminary data reveal that the vaccine elicits protective, Coccidioides endoglucanase 2 (C-Eng2) specific CD4+ T cells and that bronchiolar club cells and Ca++ calcineurin signaling in the cells are needed to mobilize inflammatory and T cells in response to vaccine. We also find that Microfold (M) cells descend from the bronchiolar club cells and facilitate T cell priming in response to the vaccine. From these preliminary data, we hypothesize that bronchiolar club cells and M cells regulate mucosal cellular immunity in response to intranasal vaccine. To test this hypothesis, we have created innovative tools: (i) transgenic mice to deplete epithelial cell subsets or their products to further define their role in inducing immunity; (ii) C-Eng2 specific tetramers to track and analyze protective CD4+ T cells and TRM in C57BL6 mice; and (iii) methods to isolate and culture human lung epithelial cells to translate results from mice to humans. We propose three aims to test our hypothesis. In Aim 1, we will elucidate early stages of the inflammatory response to intranasal vaccine regulated by bronchiolar club cells and M cells; in Aim 2, we will identify lung epithelial cell receptors - dectin-1, DUOX1 and DUOXA1 - and downstream PLCG2 that may sense intranasal vaccine and signal via Ca++ and calcineurin to mobilize mucosal immunity; and in Aim 3, we will define mechanisms of vaccine-induced durable mucosal immunity by studying lung TRM and the regulatory role of lung epithelium. In sum, we address the unmet need of vaccination against coccidioidomycosis. Our work is significant as it will define mechanisms by which a promising vaccine establishes T cell immunity at the lung mucosa. Results will identify tactics useful for other vaccine immunogens given intranasally, including subunit vaccines. The work will define correlates of immunity needed to advance this attenuated vaccine or next generation subunit vaccines against this high priority pathogen. The work will be done with state-of-the-art, cutting-edge tools. Our team of PI and Co-I’s will let us translate results from mouse to human, with tools and reagents for human lung epithelium.

摘要 球孢子菌病是一种重新出现的感染，NIH优先考虑疫苗预防。实验 活减毒孢子疫苗在鼻内递送后具有高度保护性。我们打算研究这种疫苗 定义肺上皮调节持久粘膜T细胞免疫的机制。抗日 吸入的微生物被认为存在于组织驻留记忆（TRM）细胞中，但很少有人知道这是如何发生的。 鼻内疫苗诱导肺TRM。我们的初步数据显示，疫苗具有保护性，球孢子菌 内葡聚糖酶2（C-Eng 2）特异性CD 4 + T细胞和细支气管俱乐部细胞以及Ca++钙调神经磷酸酶信号转导在 需要这些细胞来动员炎症细胞和T细胞以响应疫苗。我们还发现，微折叠（M） 细胞从细支气管俱乐部细胞下降，并促进T细胞对疫苗的反应。 从这些初步的数据，我们假设细支气管俱乐部细胞和M细胞调节粘膜， 对鼻内疫苗应答的细胞免疫。为了验证这一假设，我们创建了创新工具：（i） 转基因小鼠消耗上皮细胞亚群或其产物，以进一步确定其在诱导免疫中的作用; (ii)追踪和分析C57 BL 6小鼠中的保护性CD 4 + T细胞和TRM的C-Eng 2特异性四聚体;和（iii） 分离和培养人肺上皮细胞的方法，以将结果从小鼠转化为人类。我们提出 三是检验我们的假设。在目标1中，我们将阐明炎症反应的早期阶段， 鼻内疫苗受细支气管俱乐部细胞和M细胞调节;在目标2中，我们将鉴定肺上皮细胞 受体- dectin-1、DUOX 1和DUOXA 1-和下游PLCG 2，其可以感测鼻内疫苗， 通过Ca++和钙调神经磷酸酶的信号传导来动员粘膜免疫;在目标3中，我们将定义 通过研究肺TRM和肺上皮的调节作用，疫苗诱导持久的粘膜免疫。 总之，我们解决了球孢子菌病疫苗接种的未满足需求。我们的工作意义重大， 它将确定一种有希望的疫苗在肺粘膜建立T细胞免疫的机制。结果 将确定其他疫苗免疫原鼻内给药，包括亚单位疫苗有用的策略。工作 将确定推进这种减毒疫苗或下一代亚单位疫苗所需的免疫相关性 对抗这种高优先级病原体这项工作将用最先进的尖端工具完成。我们的团队的 PI和Co-I's将使我们能够利用用于人类肺上皮的工具和试剂将结果从小鼠转化为人类。