Defining post-transcriptional gene regulation in FMRP-deficiency usingmiRNA:target chimeras

使用 miRNA：目标嵌合体定义 FMRP 缺陷的转录后基因调控

基本信息

批准号：
10586591
负责人：
MOLLIE Katherine MEFFERT
金额：
$ 48.4万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-01-01 至 2027-11-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10586591
关键词：
Affect Behavioral Binding Proteins Biochemical Biogenesis Brain Central Nervous System Child Chimera organism Cognitive Complex Coupling Data Data Set Detection Development Diagnostic FMR1 Family Fragile X Syndrome Functional disorder Gene Expression Regulation Gene Targeting Genes Genetic Diseases Goals Growth High-Throughput Nucleotide Sequencing Human Hyperactivity Immunoprecipitation Impairment Incidence Intellectual functioning disability Intervention Investigation Knock-out Knockout Mice Knowledge Ligation Link MAP Kinase Gene MAPK3 gene Mediating Messenger RNA MicroRNAs Molecular Molecular Target Morphology Motivation Mus Neuroanatomy Neurodegenerative Disorders Neurodevelopmental Disorder Neurons Pathway interactions Patients Phenotype Post-Transcriptional Regulation Production Protein Biosynthesis Protein Deficiency Proteins RNA Binding RNA-Binding Proteins RNA-Induced Silencing Complex Repression Resistance Signal Transduction Small RNA Social Interaction Specificity Synapses Techniques Testing Time Transcript Translations Untranslated RNA Validation autism spectrum disorder behavioral phenotyping brain abnormalities cell type cognitive function crosslink excitatory neuron gene repression genetic regulatory protein genome-wide human model human stem cells in vivo induced pluripotent stem cell interest mouse model nervous system disorder neural neuronal growth posttranscriptional programs protein function protein phosphatase inhibitor-2 repetitive behavior restoration social communication

项目摘要

Fragile X Syndrome (FXS) is the most common monogenic cause of Autism spectrum disorder (ASD), a group of complex neurodevelopmental disorders characterized by core diagnostic impairments in social interactions and communication, restricted repetitive behaviors and interests, and an association with intellectual disability. FXS results from deficiency in expression of the FMR1 gene encoding Fragile X Mental Retardation Protein (FMRP). An early overgrowth of neurons and excessive immature synaptic contacts have been observed in brains of children with FXS, as well as in the Fmr1 KO mouse model. At a molecular level, aberrant excessive protein synthesis, with altered production of key synaptic proteins, is implicated in the atypical neural and synaptic overgrowth. While FMRP loss can lead to excessive protein synthesis, mechanisms altering the gene-target selectivity of protein synthesis to produce the distinct phenotypes of FMRP-deficiency are incompletely understood. MicroRNAs (miRNAS) are small RNAs which can selectively target gene transcripts for repression in the RNA- induced silencing complex (RISC). FXS has been linked to misregulation of miRNAs and miRNA-mediated gene repression for over 15 years, but broad knowledge of alterations in targeted transcripts has been lacking. We propose to carry out genome-wide quantitative comparisons of RISC-mediated gene targeting in the wildtype and FMRP-deficient setting using both mice and human neurons. Directed by preliminary data, we will investigate the candidate let-7 miRNA family to test the hypothesis that dysregulation of let-7 miRNA biogenesis in the Fmr1 KO mouse contributes to altered repression of pro-growth mRNAs and downstream behavioral and neuroanatomical phenotypes. A multipronged approach for mechanistic investigation and prioritizing gene targets and pathways from genome-wide assessments will be followed by intervention to assess the functional consequences for FXS-associated phenotypes with the goal of enhancing our understanding of FMRP function and providing new molecular targets for intervention in phenotypes resulting from deficiency of FMRP.

脆弱的X综合征（FXS）是自闭症的最常见单基因原因频谱障碍（ASD），一组复杂的神经发育障碍以社会互动中的核心诊断障碍为特征沟通，限制重复行为和利益以及协会有智力残疾。 FXS由FMR1表达不足导致编码脆弱X智障蛋白（FMRP）的基因。早期过度生长在 FXS以及FMR1 KO小鼠模型的大脑。在分子水平，异常过多的蛋白质合成，产生的产生改变关键突触蛋白与非典型神经和突触过度生长有关。虽然FMRP损失会导致蛋白质合成过多，但机制改变了蛋白质合成的基因目标选择性产生不同的表型 FMRP缺陷的缺陷尚不完全理解。 microRNA（mirnas）很小 RNA可以选择性地靶向基因转录本以抑制RNA- 诱导沉默复合物（RISC）。 FXS已与对 miRNA和miRNA介导的基因抑制已有15年以上，但广泛缺乏有关目标成绩单改变的知识。我们建议对RISC介导的基因靶向进行全基因组的定量比较在使用小鼠和人类神经元的野生型和FMRP缺陷设置中。通过初步数据指导，我们将研究候选let-7 miRNA家族为了检验以下假设，即FMR1 KO中Let-7 miRNA生物发生失调小鼠有助于改变促增长mRNA和下游的抑制作用行为和神经解剖表型。一种多收益的方法机械研究并优先考虑基因靶标和途径全基因组评估将进行干预以评估功能与FXS相关表型的后果，目的是增强我们了解FMRP功能并提供新的分子靶标 FMRP缺乏引起的表型干预。