Imaging, Histology and Functional Diagnostics Core

影像、组织学和功能诊断核心

• 批准号: 10273969
• 负责人: Janet Ruthe Sparrow
• 金额: $ 32.26万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10273969
• 关键词: 3D Print; Achievement; Adult; Angiography; Animal Disease Models; Animal Experimentation; Animal Model; Animals; Area; Brain; Buffers; Clinical; Clinical Research; Collaborations; Color; Computer software; Core Facility; Core Grant; Cornea; Development; Diagnostic; Diagnostic tests; Disease; Doctor of Philosophy; Electroretinography; Embryo; Ensure; Equipment; Equipment and Supplies; Eye; Faculty; Film; Fixatives; Floor; Funding; Fundus; Glaucoma; Goals; Histologic; Histological Techniques; Histology; Human Resources; Human Subject Research; Image; Imaging Device; Individual; Institutes; Investigation; Knowledge; Laboratory Technicians; Lasers; Learning; Letters; Light; Maintenance; Microscopic; Microtome - medical device; Mission; Modality; Molds; Monkeys; Mus; Neonatal; Nursing Faculty; Ophthalmology; Ophthalmoscopes; Optical Coherence Tomography; Oryctolagus cuniculus; Paraffin; Pathology; Patients; Pattern; Photoreceptors; Postdoctoral Fellow; Preparation; Procedures; Productivity; Protocols documentation; Rattus; Research; Research Personnel; Resolution; Resources; Retina; Retinal Ganglion Cells; Retirement; Rodent; Role; Scanning; Schedule; Scientist; Services; Sparrows; Specialist; Stains; Structure; Structure of retinal pigment epithelium; Students; Study Section; System; Techniques; Testing; Thick; Thinness; Tissues; Training; Translational Research; United States National Institutes of Health; Universities; Vision; Vision research; Water; Work; animal facility; brain pathway; clinical application; cost; cryostat; equipment acquisition; experience; fundus imaging; graduate student; in vivo; in vivo imaging; instrument; instrumentation; lens; multimodality; pathogen; postnatal; programs; recruit; repaired; skills; student training; time use; tissue preparation; tissue processing; tool; translational study

Summary/Abstract The broad goals of this Core are to provide facilities for vision scientists at Columbia University. Resources are provided for imaging, histology and electroretinographic recording. The module is administered so as to ensure equipment access, technical support and personnel training. The availability of shared state-of-the-art equipment and facilities operated by trained and highly qualified technicians with oversight by vision faculty substantially increases the quality and quantity of achievements earned by research programs. Productive functioning of these core facilities will i) facilitate collaborative efforts; ii) enable the tackling of gaps in our knowledge that require multiple areas of expertise; enhance the productivity of ongoing research making research funds more profitable; and enable the development of new research strategies. The clustering of clinically applicable instruments with in the Core enhances interactions between research and clinical faculty and encourages translational research. The availability of the resources of the Core is also an advantage when recruiting new faculty. The core provides training for students and postdoctoral associates in in vivo eye imaging using modalities of short-wavelength fundus autofluorescence, near-infrared fundus autofluorescence and optical coherence tomography (OCT). Training in tissue processing and sectioning for histological analysis is also made available as is training in electroretinographic (ERG) recording of visual function. The Core supports a clinical grade animal-dedicated confocal laser scanning ophthalmoscope (Heidelberg) equipped for short-wavelength fundus autofluorescence (AF), near-infrared fundus autofluorescence, angiography and spectral domain optical coherence tomography (SD-OCT). Two new high resolution Bioptigen SD-OCT (Envisu R2210 and R4310) enable non-contact in vivo SD-OCT imaging of the eye in animal models. This capability will advance investigations of cornea, tear film, lens, glaucoma and retinal structure in both the diseased and healthy eye. The instruments enable the study of animal models of disease in parallel with human subjects research. The core maintains 3 instruments for ERG testing. These machines are used to test the function of retinal pigment epithelium (bleach protocol, c-wave), photoreceptors (scotopic and photopic serial intensities), and retinal ganglion cells in rodents. Skilled personnel under the direction of Dr. Sparrow manage day to day activities including training in the use of the instruments. Reserving and tracking of usage time and scheduling of maintenance together with other day- to-day activities are managed by iLAB software.

摘要/摘要 该核心的总体目标是为哥伦比亚大学的视觉科学家提供设施。资源有 用于成像、组织学和视网膜电图记录。该模块的管理是为了确保 设备准入、技术支持和人员培训。共享最先进的设备的可用性 以及由训练有素的高素质技术人员运营的设施，并受到视觉教师的监督 提高研究项目所取得成果的质量和数量。的生产运作 这些核心设施将 i) 促进协作努力； ii) 能够弥补我们的知识空白 需要多个领域的专业知识；提高正在进行的研究的生产力，使研究经费更多 有利可图;并促进新研究策略的制定。临床适用的聚类 核心仪器增强了研究和临床教师之间的互动，并鼓励 转化研究。核心资源的可用性在招募新人时也是一个优势 学院。 该核心课程为学生和博士后同事提供体内眼成像方面的培训，使用以下方式： 短波长眼底自发荧光、近红外眼底自发荧光和光学相干 断层扫描（OCT）。还提供组织处理和组织学分析切片方面的培训 视功能视网膜电图（ERG）记录的培训也是如此。 Core 支持临床级动物专用共焦激光扫描检眼镜（海德堡） 配备短​​波长眼底自发荧光（AF）、近红外眼底自发荧光、 血管造影和频域光学相干断层扫描（SD-OCT）。两种新型高分辨率 Bioptigen SD-OCT（Envisu R2210 和 R4310）可在动物模型中对眼睛进行非接触式体内 SD-OCT 成像。 这种能力将促进角膜、泪膜、晶状体、青光眼和视网膜结构的研究 患病和健康的眼睛。这些仪器能够与人类疾病模型同时进行动物模型研究 课题研究。 核心维护 3 台 ERG 测试仪器。这些机器用于测试视网膜的功能 色素上皮（漂白方案、c 波）、光感受器（暗视和明视系列强度）和 啮齿动物的视网膜神经节细胞。 Sparrow 博士指导下的熟练人员管理日常活动，包括使用培训 仪器。保留和跟踪使用时间以及与其他日期一起安排维护- 今天的活动由 iLAB 软件管理。