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Imaging, Histology and Functional Diagnostics Core

影像、组织学和功能诊断核心

基本信息

批准号：
10273969
负责人：
Janet Ruthe Sparrow
金额：
$ 32.26万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-07-01 至 2026-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10273969
关键词：
3D Print Achievement Adult Angiography Animal Disease Models Animal Experimentation Animal Model Animals Area Brain Buffers Clinical Clinical Research Collaborations Color Computer software Core Facility Core Grant Cornea Development Diagnostic Diagnostic tests Disease Doctor of Philosophy Electroretinography Embryo Ensure Equipment Equipment and Supplies Eye Faculty Film Fixatives Floor Funding Fundus Glaucoma Goals Histologic Histological Techniques Histology Human Resources Human Subject Research Image Imaging Device Individual Institutes Investigation Knowledge Laboratory Technicians Lasers Learning Letters Light Maintenance Microscopic Microtome - medical device Mission Modality Molds Monkeys Mus Neonatal Nursing Faculty Ophthalmology Ophthalmoscopes Optical Coherence Tomography Oryctolagus cuniculus Paraffin Pathology Patients Pattern Photoreceptors Postdoctoral Fellow Preparation Procedures Productivity Protocols documentation Rattus Research Research Personnel Resolution Resources Retina Retinal Ganglion Cells Retirement Rodent Role Scanning Schedule Scientist Services Sparrows Specialist Stains Structure Structure of retinal pigment epithelium Students Study Section System Techniques Testing Thick Thinness Tissues Training Translational Research United States National Institutes of Health Universities Vision Vision research Water Work animal facility brain pathway clinical application cost cryostat equipment acquisition experience fundus imaging graduate student in vivo in vivo imaging instrument instrumentation lens multimodality pathogen postnatal programs recruit repaired skills student training time use tissue preparation tissue processing tool translational study

项目摘要

Summary/Abstract The broad goals of this Core are to provide facilities for vision scientists at Columbia University. Resources are provided for imaging, histology and electroretinographic recording. The module is administered so as to ensure equipment access, technical support and personnel training. The availability of shared state-of-the-art equipment and facilities operated by trained and highly qualified technicians with oversight by vision faculty substantially increases the quality and quantity of achievements earned by research programs. Productive functioning of these core facilities will i) facilitate collaborative efforts; ii) enable the tackling of gaps in our knowledge that require multiple areas of expertise; enhance the productivity of ongoing research making research funds more profitable; and enable the development of new research strategies. The clustering of clinically applicable instruments with in the Core enhances interactions between research and clinical faculty and encourages translational research. The availability of the resources of the Core is also an advantage when recruiting new faculty. The core provides training for students and postdoctoral associates in in vivo eye imaging using modalities of short-wavelength fundus autofluorescence, near-infrared fundus autofluorescence and optical coherence tomography (OCT). Training in tissue processing and sectioning for histological analysis is also made available as is training in electroretinographic (ERG) recording of visual function. The Core supports a clinical grade animal-dedicated confocal laser scanning ophthalmoscope (Heidelberg) equipped for short-wavelength fundus autofluorescence (AF), near-infrared fundus autofluorescence, angiography and spectral domain optical coherence tomography (SD-OCT). Two new high resolution Bioptigen SD-OCT (Envisu R2210 and R4310) enable non-contact in vivo SD-OCT imaging of the eye in animal models. This capability will advance investigations of cornea, tear film, lens, glaucoma and retinal structure in both the diseased and healthy eye. The instruments enable the study of animal models of disease in parallel with human subjects research. The core maintains 3 instruments for ERG testing. These machines are used to test the function of retinal pigment epithelium (bleach protocol, c-wave), photoreceptors (scotopic and photopic serial intensities), and retinal ganglion cells in rodents. Skilled personnel under the direction of Dr. Sparrow manage day to day activities including training in the use of the instruments. Reserving and tracking of usage time and scheduling of maintenance together with other day- to-day activities are managed by iLAB software.

摘要/文摘