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Quantitative Fundus Autofluorescence in Retinal Disorders

视网膜疾病中的定量眼底自发荧光

基本信息

批准号：
10358501
负责人：
Janet Ruthe Sparrow
金额：
$ 39.29万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2023-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10358501
关键词：
Abbreviations Acute Address Adverse effects Age Age related macular degeneration Alleles Analysis of Variance Cells Characteristics Choroideremia Clinical Confidence Intervals Dark Adaptation Data Diagnosis Disease Disease Progression Ethnic Origin Eye Female Fluorescence Funding Fundus Gender Genetic Genotype Glyoxal Human Hydroxychloroquine Image Individual Inferior Influentials Investigation Lasers Legal Blindness Lesion Light Link Lipofuscin Liquid Chromatography Measures Medical Genetics Melanins Membrane Methods Monitor Mosaicism Multimodal Imaging Mutation Natural History Normal Range Nuclear Ophthalmoscopy Optical Coherence Tomography Outcome Measure Participant Pathogenicity Patients Pattern Performance Phenotype Photoreceptors Predisposition Process Progressive Disease Public Health Pyruvaldehyde Rab escort protein Retina Retinal Degeneration Retinal Diseases Retinitis Pigmentosa Retinol dehydrogenase Scanning Serous Signal Transduction Source Spatial Distribution Spectrometry, Mass, Electrospray Ionization Standardization Stargardt&apos s disease Structure of retinal pigment epithelium Testing Therapeutic Thick Toxic effect Visual Acuity Work aged clinical diagnosis confocal imaging disorder of macula of retina fundus imaging genetic testing interest macula mutation carrier retinal imaging therapy outcome

项目摘要

Description: Project Summary and Relevance The inherent autofluorescence (AF) of the fundus originates from RPE bisretinoid lipofuscin. While RPE lipofuscin is amassed even in healthy eyes, imaging of fundus AF by confocal scanning laser ophthalmoscopy (cSLO) has shown that the patterns and intensities of AF deviate from normal in several retinal disorders. Accordingly, we previously demonstrated that a standardized approach to quantifying short wavelength fundus AF (qAF) can assist in the diagnosis of retinal disease, in the monitoring of disease progression and in the assessment of therapeutic outcomes. To enable these investigations we have gathered normative qAF data from a large number of participants with healthy eye status (aged 6-60) so as to establish ranges of qAF values with respect to age, gender and ethnicity. Going forward we will use these normal values to determine whether bisretinoid lipofuscin accumulation, a cellular burden that distinguishes RPE cells, confers susceptibility to the adverse effects of HCQ on retina and whether qAF levels in inferior macula can serve as an early sign of HCQ toxicity (Specific Aim 1). We will also determine why female carriers of choroideremia, present with reduced RPE lipofuscin measured as qAF (Specific Aim 2). Using the qAF approach we will test for a relationship between the loss of the ellipsoid zone in SD-OCT images and changes in short wavelength fundus autofluorescence (SW-AF) intensities in patients diagnosed with central serous chorioretinopathy (Specific Aim 3). Homozygous mutations in ABCA4 are well known to confer elevated qAF although the spatial distribution of the fluorescence intensity is not uniform. By multi-modal imaging we will elucidate the underlying structural basis of this topographic distribution (Specific Aim 4). In summary, the studies proposed in this application will examine the contribution that the lipofuscin of retina makes to the onset and progression of several retinal diseases and will demonstrate that quantitation of fundus AF facilitates the diagnosis and monitoring of some retinal disorders.

描述：项目总结和相关性