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Adhesion Screening of Dental Implant Materials Using Laser-Driven Acoustic Waves

使用激光驱动声波进行牙科植入材料的粘附力筛选

基本信息

批准号：
10237137
负责人：
Martha Grady
金额：
$ 14.56万
依托单位：
UNIVERSITY OF KENTUCKY
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-12 至 2023-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10237137
关键词：
Acoustics Adhesions Adhesives Antibiotics Area Atomic Force Microscopy Bacteria Bacterial Adhesion Basic Science Biocompatible Materials Biological Biological Testing Calibration Cell Adhesion Cell Count Cells Characteristics Clinical Clinical Sciences Collaborations Communities Data Dental Implants Disease Engineering Environment Evaluation Film Future Goals Growth Human Implant Infection Investigation Kentucky Lasers Light Maintenance Mammalian Cell Measurement Measures Mechanics Medical Medical Device Medical Technology Metabolic Methods Microbial Biofilms Modification Molecular Oral Oral cavity Organism Osseointegration Osteoblasts Outcome Patients Physiologic pulse Physiological Polymers Population Procedures Protocols documentation Recurrence Reporting Research Resolution Safety Saliva Scheme Scientist Side Streptococcus Streptococcus gordonii Streptococcus mutans Streptococcus oralis Streptococcus sanguis Stress Sucrose Surface System Techniques Testing Titanium Translational Research Universities Work absorption base clinical center cohesion design implant material implantable device improved indexing infection risk innovation innovative technologies interest interfacial nanoscale nanosecond novel oral microbial community peri-implantitis preclinical evaluation promoter screening stem technology validation tool translational physician treatment response

项目摘要

ABSTRACT Bacteria accumulation on implants and devices within the oral cavity puts a patient at serious risk for infection. Eradication of established biofilm-forming infections remains difficult, in part because the accumulated bacteria are physiologically and metabolically distinct from the planktonic cells of the same organism. Despite intense efforts in the field, biofilm level response to treatments and changes in environment has been hindered by the lack of robust, quantitative, and accurate biofilm characterization techniques that can be directly correlated to implant surfaces. Accurate biofilm adhesion measurement techniques are necessary because strong biofilm adhesion contributes to biofilm persistence on medical device surfaces. In order to more accurately assess the characteristics and contribution of biofilm adhesion on implant infections, an appropriate film adhesion measurement technique must be developed for evaluation of low cohesive strength-high adhesive strength biofilm-substrate interfaces. The proposed investigation is designed to overcome current limitations in biofilm adhesion measurement techniques by harnessing laser-induced acoustic waves. We recently discovered a new technique that generates stress waves from a pulsed laser is capable of separating a biofilm from a surface. This discovery is especially important for biofilms with low cohesive strengths because existing measurement techniques perform poorly on these biofilms, whereas, our newly discovered technique is well-suited for characterizing these types of biofilms. Controlling the amplitude of the generated stress waves from a pulsed laser enables a direct comparison of the stress required for biofilm detachment. We will assess and optimize the laser spallation technique for quantitative biofilm adhesion strength measurement, evaluate changes in adhesion due to surface treatments (e.g., roughness, polymer coating). Successful completion of this research results in new tools for quantitative adhesion measurement of biofilm-surface interfaces. Implementation of this technology will enable rational selection of implant materials for reduced biofilm adhesion and improved clinical outcomes.

摘要细菌在口腔内的植入物和器械上积累，使患者面临严重的感染风险。根除已建立的生物被膜感染仍然很困难，部分原因是积累的细菌在生理和代谢上与同一生物体的浮游细胞截然不同。尽管紧张在现场的努力，生物膜水平对处理和环境变化的反应受到缺乏可靠的、定量的和准确的生物膜表征技术，可以直接与植入物表面。由于生物膜很强，因此需要准确的生物膜附着力测量技术。粘附性是医疗器械表面生物膜持久性的重要原因。为了更准确地评估种植体感染时生物膜粘连的特点及贡献必须发展测量技术来评价低粘结强度-高粘结强度生物膜-基质界面。这项拟议的研究旨在克服目前生物膜粘附性测量的局限性。通过利用激光诱导的声波的技术。我们最近发现了一种新技术，可以产生来自脉冲激光的应力波能够将生物膜从表面分离出来。这一发现尤其是对于低结合强度的生物膜很重要，因为现有的测量技术在然而，我们新发现的技术非常适合描述这些类型的生物膜。通过控制脉冲激光产生的应力波的幅度，可以直接比较生物膜脱离所需的压力。我们将对激光层裂技术进行评估和优化，以生物膜附着强度测量，评估由于表面处理而引起的附着变化(例如，粗糙度、聚合物涂层)。这项研究的成功完成为量化研究提供了新的工具生物膜-表面界面附着力的测量。这项技术的实施将使Rational 用于减少生物膜粘连和改善临床结果的植入材料的选择。