Center for Epigenomics of the Mouse Brain Atlas (CEMBA)

小鼠大脑图谱表观基因组学中心 (CEMBA)

• 批准号: 10252523
• 负责人: EDWARD M CALLAWAY
• 金额: $ 36.19万
• 依托单位: SALK INSTITUTE FOR BIOLOGICAL STUDIES
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-20 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10252523
• 关键词: ATAC-seq; Algorithms; Atlases; BRAIN initiative; Behavioral; Brain; Brain region; Catalogs; Categories; Cell Nucleus; Cells; Censuses; Chromatin; Classification; Communities; Computational algorithm; DNA Methylation; Data; Development; Dissection; Distal; Elements; Enhancers; Epigenetic Process; Forebrain Development; Freezing; Genetic; Genetic Fingerprintings; Genome; Genomics; Goals; Human; Image; Individual; Joints; Label; Laboratory mice; Location; Methods; Methylation; Mitotic; Modeling; Modification; Molecular; Molecular Profiling; Mus; Nature; Neuroglia; Neuronal Differentiation; Neurons; Nucleic Acid Regulatory Sequences; Pattern; Phase; Physiological; Production; Regulator Genes; Regulatory Element; Research; Research Personnel; Resolution; Rodent; Techniques; Tissues; Transgenic Mice; Type I Epithelial Receptor Cell; Viral; base; brain cell; brain tissue; cell type; computational pipelines; cost effectiveness; epigenome; epigenomics; excitatory neuron; experience; human disease; inhibitory neuron; methylation pattern; methylome; neural circuit; neurochemistry; single cell analysis; software development; tool; transcriptomics

Abstract Understanding the exact cell-type composition in the different regions of the mouse brain is a fundamental step when trying to integrate physiological, behavioral, neurochemical and molecular data. At present, although major categories of cell-types present in the mouse brain have been defined through a handful of specific markers, the different subtypes within these categories, as well as their location and connectivity are far from understood. Epigenomic signatures such as DNA methylation (mC) and open chromatin are stable modifications that persist in post-mitotic cells throughout their lifetime, defining their cellular identity. Open chromatin as well as methylation patterns are cell type specific, differentiating the major types of cells i.e., neurons and glia, in the rodent and human cortex, as well as differentiating neuronal types in mouse brain. Research Segment 1 of this center proposes to produce a catalog of methylation and open chromatin patterns at the single-cell level throughout the entire mouse brain. Analysis of the combined data will permit the discovery of cell-type specific regulatory regions that will allow the production of transgenic mouse lines and viral tools that will become available to the community.

摘要

项目成果

Cell type-specific enrichment of somatic aneuploidy in the mammalian brain.

哺乳动物大脑中体细胞非整倍性的细胞类型特异性富集。

• DOI: 10.1101/2023.12.18.572285
• 发表时间: 2023
• 期刊: bioRxiv : the preprint server for biology
• 作者: Mukamel,EranA;Liu,Hanqing;Behrens,MMargarita;Ecker,JosephR
• 通讯作者: Ecker,JosephR

Single-cell analysis of chromatin accessibility in the adult mouse brain.

• DOI: 10.1038/s41586-023-06824-9
• 发表时间: 2023-12
• 期刊: NATURE
• 影响因子: 64.8
• 作者: Zu, Songpeng;Li, Yang Eric;Wang, Kangli;Armand, Ethan J.;Mamde, Sainath;Amaral, Maria Luisa;Wang, Yuelai;Chu, Andre;Xie, Yang;Miller, Michael;Xu, Jie;Wang, Zhaoning;Zhang, Kai;Jia, Bojing;Hou, Xiaomeng;Lin, Lin;Yang, Qian;Lee, Seoyeon;Li, Bin;Kuan, Samantha;Liu, Hanqing;Zhou, Jingtian;Pinto-Duarte, Antonio;Lucero, Jacinta;Osteen, Julia;Nunn, Michael;Smith, Kimberly A.;Tasic, Bosiljka;Yao, Zizhen;Zeng, Hongkui;Wang, Zihan;Shang, Jingbo;Behrens, M. Margarita;Ecker, Joseph R.;Wang, Allen;Preissl, Sebastian;Ren, Bing
• 通讯作者: Ren, Bing