Center for Epigenomics of the Mouse Brain Atlas (CEMBA)

摘要 了解小鼠大脑不同区域的确切细胞类型组成是最基本的一步 在尝试整合生理、行为、神经化学和分子数据时。目前，虽然 小鼠大脑中存在的细胞类型的主要类别是通过几个特定的 标记，这些类别中的不同子类型，以及它们的位置和连通性远远不同 明白了。表观基因组特征，如DNA甲基化(MC)和开放染色质是稳定的 在有丝分裂后细胞一生中持续存在的修饰，定义了它们的细胞身份。打开 染色质和甲基化模式是特定于细胞类型的，区分主要类型的细胞，即， 啮齿类动物和人类皮质中的神经元和神经胶质细胞，以及小鼠大脑中分化出的神经元类型。 该中心的研究部分1建议制作甲基化和开放染色质模式的目录 在整个小鼠大脑的单细胞水平上。对合并数据的分析将允许 发现细胞类型特定的调节区，将允许生产转基因小鼠品系和 将向社区提供病毒式工具。

Abstract Understanding the exact cell-type composition in the different regions of the mouse brain is a fundamental step when trying to integrate physiological, behavioral, neurochemical and molecular data. At present, although major categories of cell-types present in the mouse brain have been defined through a handful of specific markers, the different subtypes within these categories, as well as their location and connectivity are far from understood. Epigenomic signatures such as DNA methylation (mC) and open chromatin are stable modifications that persist in post-mitotic cells throughout their lifetime, defining their cellular identity. Open chromatin as well as methylation patterns are cell type specific, differentiating the major types of cells i.e., neurons and glia, in the rodent and human cortex, as well as differentiating neuronal types in mouse brain. Research Segment 1 of this center proposes to produce a catalog of methylation and open chromatin patterns at the single-cell level throughout the entire mouse brain. Analysis of the combined data will permit the discovery of cell-type specific regulatory regions that will allow the production of transgenic mouse lines and viral tools that will become available to the community.