Bat pluripotent stem cells as a novel experimental system

蝙蝠可能是最令人着迷但最不为人所知的哺乳动物目。很明显 尽管蝙蝠是一种急需的新模式生物，但接触动物的机会有限 细胞模型阻碍了他们的研究。我们最近产生了第一个诱导的 从蝙蝠(R.ferruequum)中分离出多能干细胞(IPSCs)，发现蝙蝠 IPSCs具有独特的转录谱，富含代谢和免疫 生酮代谢途径和独特的炎症体反应。我们计划 生成额外的以测试我们的新协议是否可以用于任何蝙蝠物种 仅在蝙蝠细胞中唯一表达的必要代谢基因，包括HMGC2，可以 使用CRISPR/CAS9被击倒。我们还将评估炎症反应是否 在来自BAT IPSCs的髓系细胞中发生改变。蝙蝠干细胞系的建立及分化 后代将帮助解决蝙蝠生理学最诱人的问题，包括 豁免权。

Bats are perhaps the most fascinating but least understood mammalian order. It is clear that, although bats are a critically needed new model organism, limited access to animal and cell models has hindered their study. We recently generated the first induced pluripotent stem cells (iPSCs) from a bat (R. ferrumequinum) and discovered that bat iPSCs had a unique transcriptomic profile enriched in metabolomic and immune pathways ketogenic metabolism and a unique inflammasome response. We plan to generate additional to test if our novel protocol can be used for any bat species and that essential metabolic genes uniquely expressed only in bat cells, including HMGC2, can be knocked out using CRISPR/Cas9. We will also evaluate if the inflammatory response is altered in myeloid cells derived from bat iPSCs. Bat stem cell lines and differentiated progeny will help address bat physiology’s most tantalizing questions, including immunity.