Smartphone-based POC Testing for HIV Using Glowstick Chemistry
基于智能手机的 HIV 使用荧光棒化学进行 POC 测试
基本信息
- 批准号:10594143
- 负责人:
- 金额:$ 45.65万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2023
- 资助国家:美国
- 起止时间:2023-01-06 至 2025-12-31
- 项目状态:未结题
- 来源:
- 关键词:AcidsAddressAdolescentAffinityAfrican AmericanAndroidAntibodiesAntigen-Antibody ComplexArchivesAvidityBindingBloodBullaCellular PhoneChemicalsChemistryClinicClinicalCollaborationsCommunicable DiseasesDetectionDevelopmentDiagnosticDiagnostic ProcedureDissociationEarly DiagnosisEarly treatmentEnsureEnzyme-Linked Immunosorbent AssayEpitopesFibronectinsFluorescence PolarizationFormulationGoalsHIVHIV InfectionsHIV SeropositivityHIV diagnosisHealthHealth systemHispanicHomeHumanHuman immunodeficiency virus testImmunoassayInfectionInstructionInterruptionKineticsLateralLibrariesLigandsLightLinkLiquid substanceMaterials TestingMeasurementMedicalModelingMonitorOpticsPatientsPerformancePersonsPhage DisplayPhasePopulationPopulation HeterogeneityPopulations at RiskReadabilityReadingReagentReporterResearchResourcesRiskSamplingSourceSpecimenTechnologyTestingTimeTranslatingViral Load resultVisitWorkacute infectionanalogantigen detectionantiretroviral therapybiobankcohortcostdemographicsdetection limitfallsfluorophorehigh riskimprovedinnovationinsightlateral flow assaymanufacturemembernovelparticlepediatric human immunodeficiency viruspoint of carepre-exposure prophylaxispreventprotein biomarkersquality assurancerecruitscaffoldsealself testingseroconversiontest striptransmission processusability
项目摘要
Effective testing for HIV allows for detection of acute infections (new and rebound), appropriate
antiretroviral treatment, suppression of viral load, and interruption of transmission. However, early
detection of infection or regular self-monitoring at point-of-need or at home remains a challenge. Lateral
flow assays are a well-established, inexpensive point-of-need diagnostic method, and commonly are
favored when applicable because of their ease of use and low cost. Where they most commonly fall
short is in analytical sensitivity, notably in antigen-detection diagnostics for infectious diseases. Our
goal is to develop radically-improved LFAs by implementing the inexpensive, shelf-stable
chemi-excitation chemistry of glow sticks in a point-of-care format to excite fluors without light or optical
filters. Our preliminary results suggest this chemistry enables LFAs with greatly improved limits of
detection (very low pg/mL), using a standard smartphone with a $2 adaptor and no sophisticated optical
components. Our central hypothesis is that our novel, reagent-optimized chemi-excited lateral flow
assay technology integrated with a storage-stable reagent cartridge and readable with a smartphone
will lead to a new, widely-useful and potentially very-superior class of highly-sensitive lateral flow
assays. We will test this hypothesis through 5 specific aims: (1) Development of a highly-sensitive and
easily-usable lateral flow assay for HIV p24 in human blood, including development of novel
avidity-enhanced dual-fibronectin-domain binders, (2) Immune complex dissociation (ICD) for
rebound/breakthrough HIV diagnosis, (3) Testing with 300 negative and archived-positive human
samples from diverse cohorts, (4) To evaluate clinical performance using fresh samples from patients
and usability with a diverse population of subjects at high risk of HIV infection/rebound (R33 Phase), (5)
Easier usability by more people, quality assurance for test items (R33 Phase).
对艾滋病毒的有效检测可以发现急性感染(新发和反弹),
抗逆转录病毒治疗、抑制病毒载量和阻断传播。然而,早
在需要时或在家中检测感染或定期自我监测仍然是一个挑战。横向
流动测定是一种完善的、廉价的即时诊断方法,
由于其易于使用和低成本,在适用时受到青睐。它们最常掉落的地方
不足之处在于分析灵敏度,特别是在传染病的抗原检测诊断方面。我们
我们的目标是通过实施廉价、货架稳定的
化学激发化学发光棒在一个点的护理格式激发荧光没有光或光学
filters.我们的初步结果表明,这种化学物质使LFA具有大大改善的限制,
检测(极低pg/mL),使用带有2美元适配器的标准智能手机,
件.我们的中心假设是,我们的新的,试剂优化的化学激发侧流
与储存稳定的试剂盒集成并可通过智能手机读取的分析技术
将导致一个新的,广泛有用的和潜在的非常优越的高度敏感的横向流类
分析。我们将通过5个具体目标来验证这一假设:(1)开发一种高度敏感的,
用于人血液中HIV p24的易于使用的侧流测定,包括开发新的
亲和力增强的双纤连蛋白结构域结合剂,(2)免疫复合物解离(ICD),
反弹/突破性艾滋病毒诊断,(3)对300名阴性和存档阳性人进行检测
来自不同队列的样本,(4)使用来自患者的新鲜样本评价临床性能
和HIV感染/反弹高风险的不同受试者人群的可用性(R33阶段),(5)
让更多人使用更好的可用性,测试项目的质量保证(R33阶段)。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Richard Willson其他文献
Richard Willson的其他文献
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{{ truncateString('Richard Willson', 18)}}的其他基金
Viral nanoparticles as ultrasensitive reporters in lateral-flow assays
病毒纳米粒子作为横流测定中的超灵敏报告基因
- 批准号:
8892732 - 财政年份:2015
- 资助金额:
$ 45.65万 - 项目类别:
Towards the Development of a Syndrome-specific Diagnostic Tool
开发针对特定症状的诊断工具
- 批准号:
8377050 - 财政年份:2012
- 资助金额:
$ 45.65万 - 项目类别:
Towards the Development of a Syndrome-specific Diagnostic Tool
开发针对特定症状的诊断工具
- 批准号:
8233015 - 财政年份:2011
- 资助金额:
$ 45.65万 - 项目类别:
Towards the Development of a Syndrome-specific Diagnostic Tool
开发针对特定症状的诊断工具
- 批准号:
7676538 - 财政年份:2009
- 资助金额:
$ 45.65万 - 项目类别:
THERMODYNAMIC BASIS OF ANTIBODY-ANTIGEN AFFINITY
抗体-抗原亲和力的热力学基础
- 批准号:
3468120 - 财政年份:1990
- 资助金额:
$ 45.65万 - 项目类别:
THERMODYNAMIC BASIS OF ANTIBODY-ANTIGEN AFFINITY
抗体-抗原亲和力的热力学基础
- 批准号:
2182486 - 财政年份:1990
- 资助金额:
$ 45.65万 - 项目类别:
THERMODYNAMIC BASIS OF ANTIBODY/ANTIGEN AFFINITY
抗体/抗原亲和力的热力学基础
- 批准号:
3468119 - 财政年份:1990
- 资助金额:
$ 45.65万 - 项目类别:
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