National Ferret Research and Resource Institute (NFRRI) at University of Iowa

爱荷华大学国家雪貂研究与资源研究所 (NFRRI)

• 批准号: 10596901
• 负责人: JOHN F ENGELHARDT
• 金额: $ 797.5万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10596901
• 关键词: 2019-nCoV; Affect; Biomedical Research; Biotechnology; Brain Diseases; Cell Compartmentation; Cells; Cellular biology; Contracts; Cost Sharing; Cystic Fibrosis; Disease; Drug or chemical Tissue Distribution; Enterobacteria phage P1 Cre recombinase; Equipment; Farm; Ferrets; Foundations; Funding; Future; Genetic; Genetic Engineering; Genome; Goals; Housing; Image; Institutes; Iowa; Location; Lung diseases; Microinjections; Modeling; Mus; Mutation; Nebulizer; Operative Surgical Procedures; Organ; Pancreatic Diseases; Pulmonary function tests; Research; Research Personnel; Resources; Services; Sterility; Strategic Planning; Technology; United States Department of Agriculture; Universities; Vertebral column; design; design and construction; genomic locus; human disease; human model; infectious disease model; mouse model; next generation; pre-clinical; recruit; research facility; research study; stem cells; synergism; therapy development; zygote

While genetic mouse models have been the backbone of translational biomedical research for the past four decades, mice often fail to model human disease due to species-specific differences in the cell biology of the affected organs, as well as the evolutionary divergence of their genome. The ferret has proven an excellent species for modeling infectious disease (including SARS-CoV2) and human diseases of the brain, pancreas, and lung when mice have failed. The University of Iowa (UI) is the only location in the world capable of genetically engineering ferrets with multiple precise genetic alterations previously only achievable in mice. This technology has enabled the construction of genetic ferret models capable of fate mapping stem cell compartments (i.e., lineage tracing), modeling human diseases including those with humanized genetic loci, and conditional genetics using Cre recombinase. A federally-funded National Ferret Research and Resource Center was formed eight years ago, which provides services for the creation of new genetic ferret models, performing research studies in ferrets, and the distribution of tissues and cells from existing genetic lines. This national resource has provided services to more than 129 academic investigators,108 of which are outside the UI, and currently has 8 contracts with biotechnology companies seeking to develop therapies for genetic and acquired diseases using ferret models. These services have exceeded the capacity of facilities for both performing research in ferrets and housing ferrets on the UI campus. This proposal seeks to design and construct a research facility that will house the National Ferret Research and Resource Institute (NFRRI) on the UI campus. The proposed facility has been designed to maximize synergy and minimize overlap with existing facilities at UI and will be adjacent and connected to existing ferret expansion housing (referred to as the BSRF facility). The NFRRI will contain state- of-the-art equipment for genetically engineering ferret zygotes and performing research in ferrets. Procedural space will include a USDA-compliant sterile surgical suite and separate microinjection room for model creation. In addition to wet lab research space, specialized procedural suites will accommodate nebulization, pulmonary function testing, and specialized imaging equipment. The NFRRI will primarily provide services to academic investigators outside UI and for-profit biotechnology companies, but will also provide ferret models for research that will occur on the main UI campus in existing research facilities. Furthermore, the chosen location for the facility has adjacent land for future expansion of research space and ferret housing with a long-term strategic plan that recruits next-generation leaders of the NFRRI and capitalizes on existing relationships with for-profit biotechnology companies seeking to develop therapies for human diseases in preclinical ferret models. The short- and long-term strategic goals of the NFRRI will be facilitated through financial partnerships with the University of Iowa (cost sharing of the NFRRI building), Cystic Fibrosis Foundation, and Marshall Farms (the largest breeder of ferrets for research).

尽管遗传小鼠模型一直是过去四个转化生物医学研究的骨干 几十年来，由于物种特异性的细胞生物学差异，小鼠通常无法模拟人类疾病 受影响的器官以及其基因组的进化差异。雪貂证明很棒 用于建模传染病的物种（包括SARS-COV2）和大脑的人类疾病，胰腺， 当老鼠失败时肺。爱荷华大学（UI）是世界上唯一具有遗传能力的地点 具有多种精确遗传改变的工程雪貂先前仅在小鼠中才能实现。这项技术 已经实现了能够命运映射干细胞室的遗传雪貂模型的构建（即 谱系追踪），对包括人性化遗传基因座和条件遗传学的人类疾病进行建模 使用CRE重组酶。由联邦资助的国家雪貂研究和资源中心组成了八个 几年前，为创建新的遗传雪貂模型提供服务，从事研究 雪貂，以及现有遗传线的组织和细胞的分布。这个国家资源提供了 为129多名学术研究人员提供服务，其中108位在UI之外，目前有8个合同 生物技术公司试图使用雪貂开发遗传和获得性疾病的疗法 型号。这些服务超出了在雪貂和 在UI校园内的住房雪貂。该建议旨在设计和建造一个将容纳的研究机构 UI校园的国家雪貂研究与资源研究所（NFRRI）。拟议的设施已经 旨在最大化协同作用并最大程度地减少与UI现有设施的重叠，并将毗邻，并且 连接到现有的雪貂扩展外壳（称为BSRF设施）。 NFRRI将包含状态 - 用于基因工程雪貂Zygotes和在雪貂中进行研究的设备。程序 空间将包括一个符合USDA的无菌手术套件和单独的微注射室进行模型创建。 除了湿实验室研究空间外，专门的程序套房还可以容纳雾化，肺部 功能测试和专业成像设备。 NFRRI将主要为学术提供服务 UI和营利性生物技术公司以外的调查人员，但也将为研究提供雪貂模型 这将发生在现有研究设施的主要UI校园上。此外，选择的位置 设施拥有邻近的土地，可将未来的研究空间和雪貂住房扩建，并具有长期战略性 计划招募NFRRI的下一代领导人，并利用与营利性的现有关系 试图在临床前雪貂模型中开发人类疾病疗法的生物技术公司。这 NFRRI的短期和长期战略目标将通过与财务合作伙伴关系促进 爱荷华大学（NFRRI大楼的成本分担），囊性纤维化基金会和马歇尔农场（ 雪貂的最大种种饲养者）。