Kansas Center for Metabolism and Obesity REsearch (KC-MORE) - Metabolism Core

堪萨斯代谢与肥胖研究中心 (KC-MORE) - 代谢核心

• 批准号: 10598029
• 负责人: Paige C Geiger
• 金额: $ 29.71万
• 依托单位: UNIVERSITY OF KANSAS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2027-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10598029
• 关键词: Adipocytes; Adipose tissue; Behavior; Behavioral; Bioenergetics; Biological Models; Body Composition; Body measure procedure; Carbohydrates; Cell Size; Cells; Cellular Metabolic Process; Chronic; Chronic Disease; Core Facility; Coupling; Diet; Dietary intake; Disease; Energy Intake; Energy Metabolism; Environmental Risk Factor; Equipment; Exercise; Fasting; Fatty Acids; Fatty acid glycerol esters; Food Intake Regulation; Genus Hippocampus; Glucose; Homeostasis; Human; Impairment; Individual; Insulin Resistance; Isotope Labeling; Isotopes; Kansas; Liver; Macronutrients Nutrition; Measurement; Measures; Mediating; Metabolic; Metabolic Diseases; Metabolic dysfunction; Metabolism; Methodology; Mission; Mitochondria; Mus; Muscle; Obesity; Obesity associated disease; Outcome Measure; Oxygen Consumption; Palmitates; Pancreas; Participant; Pathologic; Physical activity; Play; Pre-Clinical Model; Predisposition; Rattus; Reactive Oxygen Species; Research; Research Personnel; Resources; Rodent; Rodent Model; Role; Sampling; Scientist; Services; Sleep; System; Temperature; Testing; Thermogenesis; Time; Time-restricted feeding; Tissues; Tracer; Treatment Protocols; Weight Gain; design; diet and exercise; dietary manipulation; energy balance; human model; in vivo; insulin tolerance; metabolic abnormality assessment; metabolic phenotype; new therapeutic target; obesity development; oxidation; pre-clinical; tool; translational study

METABOLISM CORE: PROJECT SUMMARY Metabolism plays a fundamental role in the development of obesity and the pathological consequences of obesity that lead to chronic disease. Dysregulation of energy balance and impaired storage or oxidation of substrates are fundamental features of developing obesity and metabolic disease. Metabolic dysregulation is driven by both alterations in cellular mitochondrial function and disruptions in whole body metabolic homeostasis. Therefore, metabolic research that connects cellular mitochondrial energetics to whole body in vivo substrate metabolism and energy expenditure is critical for understanding mechanisms underlying metabolic dysfunction, obesity, and obesity-related disease states. The Metabolism (MET) core will provide the Kansas Center for Metabolism and Obesity REsearch (KC-MORE) with expertise, methodologies, and equipment for the study of “metabolism” from the cell to the whole body. Core missions of the MET core will be to develop a new central MET core facility for pre-clinical Models (rodents) that provides a host of key measurements, allowing for thorough metabolic phenotyping. These approaches will include expertise and equipment to quantify real time measures of in-vivo energy metabolism, substrate metabolism, food intake regulation, and glucose/insulin tolerance testing along with non-invasive measures of body composition in pre-clinical rodent models (rat and mice). A second core feature of the MET core will be to provide resources to investigators to quantify mitochondrial energetics in cells and tissues from both rodent and human samples. Finally, the MET core will also have the capability to perform metabolic isotope methodologies (isotopic labeled glucose, fatty acids, etc.) for use in humans or rodent models (in-vivo or ex-vivo) to quantify overall metabolic flux. In summary, the MET core services will be organized to provide state-of-the art core services to investigators studying metabolism in pre-clinical rodent model systems and to translational measures of mitochondrial energetics and stable metabolic isotopes for use in cells, tissues, rodents, and human participants. The MET core is also designed to largely parallel the capabilities of the Human Energy Balance core so that scientists in the KC-MORE can conduct translational studies in both rodents and humans.

新陈代谢核心：项目总结 新陈代谢在肥胖的发展和肥胖的病理后果中起着基础性的作用。 这会导致慢性病。能量平衡失调，底物储存或氧化受损 是发展肥胖症和代谢性疾病的基本特征。代谢失调是由这两个因素驱动的 细胞线粒体功能的改变和全身代谢动态平衡的破坏。因此， 将细胞线粒体能量学与体内底物代谢联系起来的代谢研究 能量消耗对于理解代谢功能障碍、肥胖和 肥胖相关的疾病状态。代谢(MET)核心将为堪萨斯新陈代谢中心和 肥胖研究(KC-MORE)，具有专业知识、方法和设备，用于研究来自 整个身体的细胞。气象中心的核心任务将是开发一个新的中央气象中心核心设施 临床前模型(啮齿动物)，提供大量关键测量，允许彻底代谢 表型鉴定。这些方法将包括专门知识和设备，以量化体内的实时测量 能量代谢、底物代谢、食物摄入调节和葡萄糖/胰岛素耐量试验 在临床前啮齿动物模型(大鼠和小鼠)中进行非侵入性身体成分测量。第二个核心 MET核心的特点将是为研究人员提供资源，以量化细胞中的线粒体能量学 以及来自啮齿动物和人类样本的组织。最后，MET核心还将具有执行以下任务的能力 代谢同位素方法(同位素标记的葡萄糖、脂肪酸等)用于人类或啮齿动物模型 (体内或体外)以量化整体代谢流量。总而言之，MET的核心服务将进行组织，以 为研究临床前啮齿动物模型系统新陈代谢的研究人员提供最先进的核心服务 以及线粒体能量学和稳定代谢同位素的翻译测量，用于细胞、组织、 啮齿动物和人类参与者。MET的核心也被设计成在很大程度上与人类的能力相媲美 能量平衡核心，以便KC-MORE的科学家可以在啮齿动物和 人类。