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Novel insights from a divergent form of germ plasm

来自不同形式种质的新见解

基本信息

批准号：
10599874
负责人：
Alexey Arkov
金额：
$ 30.61万
依托单位：
UNIVERSITY OF ILLINOIS AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10599874
关键词：
Anterior Architecture Binding Biological Models Biological Process Cell Nucleus Cell physiology Cells Cytoplasm Cytoplasmic Granules Disease Drosophila genus Drosophila melanogaster Elements Embryo Embryonic Development Event Gene Expression Germ Goals Health Human In Situ Hybridization Individual Knowledge Maintenance Mediating Medical Messenger RNA Modeling Molecular Morphogenesis Morphology Movement Neurodegenerative Disorders Organelles Particle Size Pathology Pattern Phase Phenotype Process Property Proteins Proteomics RNA RNA-Protein Interaction Regulation Ribonucleoproteins Series Solid Specific qualifier value Stretching Structure Structure of primordial sex cell Subcellular structure Testing Translational Regulation Translations Wasps Work cell type crosslink egg fly high resolution imaging in vivo insight migration novel particle single molecule transcriptome sequencing

项目摘要

Ribonucleoproteins (RNPs) are crucial elements in many biological processes and cell types. The polar granules of Drosophila melanogaster have long served as a model system for understanding the functions of RNPs. Polar granules are small, heterogeneous RNPs that specify the primordial germ cells in the fly. In this proposal, we will build upon the Drosophila knowledge using our wasp model system Nasonia vitripennis. The Nasonia equivalent of the polar granules is in the form of a large, spherical, solid RNP called the oosome. This is in contrast to the polar granules, which take the form of many small particles localized to the posterior pole of the embryo. The Nasonia oosome undergoes a dramatic migration within the posterior half of the embryo, a process which has not counterpart in Drosophila. Finally, the oosome is extruded in one very large bud that will divide and give rise to the pole cells. This is in strong contrast to pole cell formation in Drosophila, which is driven by the migration of nuclei to the posterior cortex, where individual cells form from small buds individually. The goal of this proposal is to understand the molecular and mechanistic properties of the oosome that are the basis for its morphological and functional differences from the polar granules. We will characterize the protein composition of the oosome, detail how translation is regulated within the oosome, and reveal how mRNAs and proteins are distributed throughout the oosome. We will then identify the interactions among proteins that may be important for the unique structure and function of the oosome. Finally, we will take in depth approaches to characterize the functions of novel components of the oosome. At the conclusion of this project, we will have a mechanistic understanding how the composition of the oosome and the interactions among the component molecules give rise to a novel form of RNP.

核糖核蛋白（RNPs）是许多生物过程和细胞的重要组成部分