Impact of interferon λ signaling on lung macrophage function and type 17 immunity during bacterial super-infection following influenza

流感后细菌重复感染期间干扰素α信号对肺巨噬细胞功能和17型免疫的影响

• 批准号: 10602685
• 负责人: Danielle Antos
• 金额: $ 4.77万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2026-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10602685
• 关键词: Activities of Daily Living; Affect; Alveolar; Antigen Presentation; Antigen-Presenting Cells; Attenuated; Bacterial Antigens; Bacterial Infections; Benefits and Risks; Biological Assay; CD4 Positive T Lymphocytes; Cause of Death; Cells; Complication; Data; Dendritic Cells; Environment; Enzyme-Linked Immunosorbent Assay; Epithelial Cells; Epithelium; Exhibits; Family; Flow Cytometry; Foundations; Future; Human; IFNGR1 gene; IL17 gene; Immune; Immune response; Immunity; Impairment; In Vitro; Incubated; Infection; Inflammatory; Influenza; Interferon Type I; Interferon Type II; Interferon alpha; Interferons; Label; Laboratories; Lung; Lung diseases; Macrophage; Measures; Methods; Morbidity - disease rate; Mucous Membrane; Mus; Myelogenous; Myeloid Cells; Outcome; Ovum; Pathway interactions; Phagocytosis; Population; Predisposition; Prevalence; Process; Production; Recombinant Interferon; Resolution; Respiratory System; Role; Secondary to; Severities; Signal Pathway; Signal Transduction; Staphylococcus aureus; Staphylococcus aureus infection; Syndrome; Therapeutic; Viral; Visual; Wild Type Mouse; attenuation; cell type; cytokine; experimental study; extracellular; functional outcomes; in vitro Assay; in vivo; influenza infection; insight; interleukin-22; interstitial; lung injury; methicillin resistant Staphylococcus aureus; monocyte; mortality; neutrophil; pathogen; receptor; receptor expression; recruit; respiratory pathogen; response; superinfection; uptake

Abstract Lung diseases and syndromes caused by respiratory pathogens represent a leading cause of death worldwide. Each year, influenza infections result in a significant number of fatalities, a majority of which are complicated by secondary bacterial super-infection. Primary influenza infection has been shown to increase susceptibility to secondary methicillin-resistant Staphylococcus aureus (MRSA) infection by altering the pulmonary host immune response and damaging the lung epithelial barrier, leading to increased morbidity and mortality. Macrophages, both lung-resident and those recruited to the lung, are important in super-infection resolution as they engulf, degrade, and present bacterial antigen to adaptive immune cells, ultimately leading to activation of type 17 cells, which promote pathogen clearance. While the role of interferon-(IFN)α/β during super-infection has been well characterized, type III IFNs have not been as extensively studied within the context of the lung. Data shows that IFNα/β are involved in type 17 attenuation after primary influenza infection, which may indicate that IFNλ exhibits similar inhibitory functions due to overlapping signaling pathways, although the potential for unique functions on lung cells is unresolved. Our lab has preliminary data suggesting that administration of exogenous IFNλ during super-infection reduces bacterial uptake by neutrophils and monocyte-derived cells, but the specific cell subsets impacted are unknown. I hypothesize that lung-resident macrophages are sensitive to IFNλ and that IFNλ signaling impairs phagocytosis and the type 17 immune response during super-infection. In the proposed studies, I will determine which myeloid subsets express the IFNλ receptor (IFNLR1) and identify how intact or disrupted IFNλ signaling broadly impacts lung injury and resolution after infection. More specifically, these experiments will determine how IFNλ impacts MRSA uptake and the initiation of a type 17 immune response during super- infection. IFNλ administration has been considered for therapeutic potential, and the outcomes of this proposal will shed light on potential caveats to the use of IFNλ as a possible treatment method during super-infection. Further, demonstration of the effects of IFNλ on myeloid cells will potentially be applicable to several other settings where an antiviral interferon response is observed.

摘要 由呼吸道病原体引起的肺部疾病和综合征是全世界主要的死亡原因。 每年，流感感染都会导致相当数量的死亡，其中大多数是由 继发性细菌重叠感染。原发流感感染已被证明增加了对 耐甲氧西林金黄色葡萄球菌继发感染与肺部宿主免疫功能的关系 反应和破坏肺上皮屏障，导致发病率和死亡率增加。巨噬细胞， 无论是常驻肺部的还是被招募到肺部的，在吞噬超级感染的过程中都是重要的， 降解细菌抗原并将其呈递给适应性免疫细胞，最终导致17型细胞的激活， 促进病原体清除的物质。而干扰素α/β在双重感染中的作用一直很好 因此，III型干扰素尚未在肺脏中得到广泛的研究。数据显示， 干扰素α/β参与了流感病毒感染后17型病毒的减毒作用，提示干扰素λ表现出 相似的抑制功能，由于重叠的信号通路，尽管潜在的独特功能 肺细胞尚未分解。 我们的实验室有初步数据表明，在双重感染期间给予外源性干扰素λ可以减少 中性粒细胞和单核细胞来源的细胞对细菌的摄取，但受影响的特定细胞亚群尚不清楚。 我假设肺巨噬细胞对干扰素λ敏感，并且干扰素λ信号受损。 双重感染时的吞噬作用和17型免疫反应。在建议的研究中，我会 确定哪些髓系亚群表达干扰素λ受体，并确定干扰素λ是如何完整或被破坏的 信号广泛影响肺损伤和感染后的解决。更具体地说，这些实验将 确定干扰素λ如何影响耐甲氧西林金黄色葡萄球菌摄取和启动17型免疫反应 感染。干扰素λ的使用已经被认为具有治疗潜力，这项提议的结果是 将阐明在双重感染期间使用干扰素λ作为一种可能的治疗方法的潜在警告。 此外，干扰素λ对髓系细胞的影响的演示可能也适用于其他几种细胞。 观察到抗病毒干扰素应答的环境。