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Coordination of T cell-derived signals in intestinal epithelial barrier defense

肠上皮屏障防御中 T 细胞衍生信号的协调

基本信息

批准号：
10605830
负责人：
Christopher Garrett Wilson
金额：
$ 4.13万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-03-01 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10605830
关键词：
Adoptive Transfer Affect Antigen Presentation Automobile Driving CD4 Positive T Lymphocytes Cell Communication Cells Cessation of life Charge Child Citrobacter rodentium Collaborations Colon Complex Cytoprotection Developing Countries Diarrhea Disease Engineering Enterocytes Epithelial Cells Epithelium Escherichia coli EHEC Escherichia coli Infections Etiology Family Genes Genetic Transcription Goals Goblet Cells Host Defense Hyperplasia Immune Immune response Immunology In Situ Infant Infection Infectious colitis Inflammation Mediators Inflammatory Bowel Diseases Innovative Therapy Intestines Knowledge LGR5 gene Lead Ligands Lymphocytic choriomeningitis virus MHC Class II Genes Maintenance Mediating Metabolic Modeling Molecular Mucous Membrane Mucous body substance Mus Oranges Phase Predisposition Prevention Process Proteins Reporting Research Role STAT3 gene Signal Transduction Surface T cell regulation T cell response T cell therapy T-Lymphocyte TCR Activation Testing Therapeutic Training Transgenic Organisms Up-Regulation antigen-specific T cells antimicrobial antimicrobial peptide base cell motility cell type chemokine colonic crypt cytokine diarrheal disease effector T cell enteric infection enteric pathogen enteropathogenic Escherichia coli human model innovation insight interleukin-22 intestinal barrier intestinal crypt intestinal epithelium mortality new therapeutic target novel novel therapeutics pathogen programs receptor recruit response restraint single-cell RNA sequencing stem cells

项目摘要

PROJECT SUMMARY Although much progress has been made in the treatment and prevention of diarrheal illness over the last century, the developing world is still plagued by diarrheal pathogens, with young children being particularly susceptible to diarrhea-related deaths. The long-term goal is to better understand how the coordinated responses of both immune and non-immune cells contribute to intestinal barrier protection during enteric infection. Our group recently discovered that Th17/22 cells are uniquely charged with protection of the intestinal crypts from enteric pathogens; however, the mechanisms by which these T cells are recruited to and sustain activation of crypt intestinal epithelial cells (cIECs) are unknown. The overall objectives in this application are to (i) elucidate the molecular mechanisms involved in recruiting T cells to cIECs and (ii) determine how recruited T cells interact with cIECs to deliver protective IL-22 signals. The central hypothesis is that IFN–induced upregulation of IEC- derived T cell-recruiting chemokines and MHCII expression promotes T cell recruitment to colonic crypts and targets pathogen-specific T cell-derived IL-22 signals to cIECs, respectively. The rationale for this project is that molecules involved in both recruitment and localized delivery of IL-22 to IECs will likely yield novel targets for therapies in diarrheal illnesses and inflammatory bowel disease (IBD), since responses to enteric pathogens and etiology of IBD share overlapping immune mechanisms. The central hypothesis will be tested by pursuing two specific aims: 1) IFN signaling in Lgr5+ IECs is critical for recruiting host-protective Cxcr3+ Th1 and Th17/22 cells to the colonic crypts; and 2) pathogen-specific T cells protect intestinal crypts via MHCII-dependent, sustained IL-22 delivery to IECs. The Citrobacter rodentium (C.r) model of infectious colitis, which closely models human E. coli infections, will be used to test both aims in this proposal. In Aim I, mice with deletion of the IFNR1 in Lgr5+ IECs will be used to to test the hypothesis that IFN signaling in Lgr5+ IECs is required for upregulation of T cell-recruiting chemokines Cxcl9 and Cxcl10 and adoptive transfer of Cxcr3-deficient T cells will be used to determine if the Cxcl9/10-Cxcr3 axis is involved in recruiting CD4+ T cells to cIECs. In Aim II, mice deficient for MHCII expression on IECs will be utilized to test the requirement of antigen presentation by IECs for IL-22- mediated protection of the intestinal crypts. In addition, mice that report TCR stimulation and adoptive transfer of C.r-specific T cells will be used to test the hypothesis that antigen-specific T cell–IEC interactions are important for protection from C.r infection. The research proposed in this application is innovative because it utilizes a newly engineered C.r strain to track antigen-specific T cells in the colon, and it offers unique insights into previously unrecognized roles of Lgr5+ intestinal stem cells. The proposed research is significant because it is expected to provide novel mechanisms by which T cells and IECs cooperate to protect the host from enteric pathogens. Ultimately, such knowledge has the potential for innovative therapies for infectious colitis and IBD.

项目总结