Assessment of Behavioral Deficits in Minipig Model of Pediatric Concussion

小型猪脑震荡模型行为缺陷的评估

• 批准号: 10610655
• 负责人: Alesa Hughson Netzley
• 金额: $ 4.01万
• 依托单位: MICHIGAN STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-15 至 2024-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10610655
• 关键词: Adolescent; Adult; Age; Anatomy; Animal Model; Animals; Architecture; Area; Assessment tool; Attention; Autopsy; Behavior; Behavior assessment; Behavioral; Biological; Biological Markers; Brain; Brain Concussion; Brain Injuries; Cause of Death; Cell Transplantation; Child; Childhood; Clinical; Clinical Research; Closed head injuries; Cognition; Craniocerebral Trauma; Data; Demyelinations; Development; Devices; Diagnosis; Diagnostic; Diffuse; Disease; Domestic Pig; Early identification; Effectiveness; Electrophysiology (science); Emergency department visit; Exhibits; Faculty; Family suidae; Functional Magnetic Resonance Imaging; Functional disorder; Future; Goals; Health; Histologic; Hour; Human; Imaging Techniques; Impaired cognition; Individual; Injury; Learning; Long-Term Potentiation; Magnetic Resonance Imaging; Measures; Mechanics; Mental Depression; Mentors; Miniature Swine; Modeling; Molecular; Natural regeneration; Nervous System Trauma; Neurocognitive; Neurons; Operative Surgical Procedures; Organism; Patient Care; Patient-Focused Outcomes; Patients; Phase; Physiological; Physiology; Positioning Attribute; Procedures; Public Health; Publishing; Rattus; Recovery; Rehabilitation therapy; Reproducibility; Research; Research Personnel; Rodent; Rodent Model; Sleep disturbances; Stem cell transplant; Structure; Study models; System; Techniques; Teenagers; Therapeutic; Tissues; Training; Translating; Translational Research; Trauma; Traumatic Brain Injury; United States; Work; behavior test; bench to bedside; biomarker identification; care outcomes; career; clinically relevant; cognitive function; computerized data processing; design; disability; efficacy validation; experience; head impact; improved; improved outcome; interest; member; mild traumatic brain injury; multimodality; nerve injury; nerve stem cell; neuroimaging; neuroinflammation; nonhuman primate; novel; novel therapeutic intervention; novel therapeutics; pediatric patients; porcine model; post-doctoral training; pre-clinical; prognostication; relating to nervous system; response; skills; stroke model; translational neuroscience; transplantation therapy; white matter

Project Summary/Abstract Traumatic brain injury (TBI) is a leading health concern for both children and adults worldwide. Similarities between pig and human brain anatomy, development, and neuroinflammatory response have brought attention to the domestic pig as a highly promising model animal for studying age-specific responses to mechanical trauma. To date, I have quantified baseline behavioral data in healthy Yucatan minipigs. For the duration of the training period, I will conduct a battery of behavioral tests to identify changes in behavior and physiology in juvenile pigs following a closed-head impact procedure designed to model pediatric concussion. In the postdoctoral phase, I plan to develop expertise in the development of novel neurointervention strategies in large animal models. To facilitate transition to a lab where I will need to utilize state-of-the-art imaging techniques to validate the efficacy of my treatments, I will use magnetic resonance imaging (MRI) as an assessment tool to quantify changes in neural structure and function in brain-injured pigs. I will receive comprehensive training in MRI data processing from experts in both human and pig neuroradiology. The proposed training plan is specifically crafted to prepare me, Alesa Netzley, for a fulfilling and distinguished career as a large animal systems and translational neuroscientist.

项目概要/摘要 创伤性脑损伤 (TBI) 是全世界儿童和成人的主要健康问题。相似之处 猪和人脑之间的解剖、发育和神经炎症反应引起了人们的关注 家猪作为一种非常有前途的模型动物，用于研究年龄特异性对机械的反应 创伤。迄今为止，我已经量化了健康尤卡坦小型猪的基线行为数据。在此期间 在训练期间，我将进行一系列行为测试，以确定行为和生理的变化 幼年猪接受了旨在模拟小儿脑震荡的闭头冲击程序。在 博士后阶段，我计划在大规模开发新型神经干预策略方面发展专业知识 动物模型。为了便于过渡到实验室，我需要利用最先进的成像技术来 为了验证我的治疗效果，我将使用磁共振成像 (MRI) 作为评估工具 量化脑损伤猪的神经结构和功能的变化。我将接受全面的培训 来自人类和猪神经放射学专家的 MRI 数据处理。拟议的培训计划是 专门为我，Alesa Netzley，为大型动物的充实而杰出的职业生涯做好准备 系统和转化神经科学家。