Contact-dependent signaling and DNA transposition in Burkholderia

伯克霍尔德氏菌中的接触依赖性信号传导和 DNA 转座

基本信息

  • 批准号:
    10610358
  • 负责人:
  • 金额:
    $ 38.88万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2020
  • 资助国家:
    美国
  • 起止时间:
    2020-05-01 至 2025-04-30
  • 项目状态:
    未结题

项目摘要

Abstract Bacteria typically live in complex sociomicrobiological communities, often as biofilms, on surfaces as diverse as water pipes, ship hulls, plant roots, insects, shellfish, indwelling medical devices, and mucosal surfaces. Biofilm growth in humans can cause or exacerbate disease, and biofilm growth in environmental niches can facilitate transmission of pathogenic bacteria to humans and other animals. Understanding how bacteria recognize, cooperate and compete with their neighbors in diverse environments is critical for developing strategies to control microbiological community composition, to prevent biofilm development, and to eliminate pre-existing biofilms and their consequent diseases. Contact-Dependent Growth Inhibition (CDI) is a phenomenon in which bacteria use the toxic C-terminus of a large exoprotein to kill or inhibit the growth of neighboring bacteria upon cell-cell contact. Production of a small immunity protein protects bacteria against CDI. Using the Gram-negative bacterium Burkholderia thailandensis as a model, we have discovered that in addition to using CDI system proteins to kill their neighbors, bacteria can use these proteins for signal transduction, causing a change in gene expression that leads to the production of cooperative behaviors, such as biofilm formation, when neighboring bacteria are recognized as ‘self’, a phenomenon we call CDS (for contact-dependent signaling). We recently discovered that the genes encoding the CDI system proteins in B. thailandensis (bcpAIOB) are located on a large mobile element that defines a new class of transposon. We showed that this transposon moves using a copy-out-paste-in mechanism, that the copy-out step, which results in the formation of a large (210 kb), circular, extrachromosomal ‘megacircle’, requires the activity of the BcpA exoprotein, and that megacircle formation is required for CDS phenotypes. We now plan to determine the molecular mechanisms by which the bcpAIOB genes and the proteins they encode contribute to megacircle formation, the molecular mechanisms by which megacircle formation leads to gene expression changes resulting in cooperative behaviors, and the role this system plays in the development of sociomicrobiological community development. Understanding the function of these systems at the molecular level may lead to the development of new antibiotics, new approaches to blocking biofilm development and biofilm-mediates diseases, and new approaches to blocking transposon-mediated spread of antibiotic resistance.
抽象的 细菌通常生活在复杂的社会微生物群落中,通常以生物膜的形式存在于表面 水管、船体、植物根、昆虫、贝类、留置医疗器械等 粘膜表面。人体生物膜生长会导致或加剧疾病,生物膜生长 环境中的细菌可以促进病原菌传播给人类和其他人 动物。了解细菌如何与邻居识别、合作和竞争 多样化的环境对于制定控制微生物群落的策略至关重要 成分,以防止生物膜形成,并消除预先存在的生物膜及其 随之而来的疾病。接触依赖性生长抑制(CDI)是一种现象,其中 细菌利用大外蛋白的有毒 C 末端来杀死或抑制邻近细菌的生长 细胞与细胞接触时的细菌。产生一种小的免疫蛋白可以保护细菌免受 CDI。使用革兰氏阴性细菌泰国伯克霍尔德氏菌作为模型,我们有 发现除了使用 CDI 系统蛋白来杀死它们的邻居之外,细菌还可以使用 这些蛋白质用于信号转导,引起基因表达的变化,从而导致 当邻近的细菌处于合作状态时,就会产生合作行为,例如生物膜的形成 被识别为“自我”,我们将这种现象称为 CDS(接触依赖信号传导)。我们最近 发现 B. thailandensis (bcpAIOB) 中编码 CDI 系统蛋白的基因是 位于定义一类新转座子的大型移动元件上。我们证明了这 转座子使用复制粘贴机制移动,即复制步骤,这会导致 形成一个大的(210 kb)、圆形、染色体外“巨环”,需要以下活性: BcpA 外蛋白,巨环形成是 CDS 表型所必需的。我们现在计划 确定 bcpAIOB 基因及其蛋白质的分子机制 编码有助于巨环形成,巨环形成的分子机制 形成导致基因表达变化,从而导致合作行为,并且这种作用 系统在社会微生物群落的发展中发挥着作用。理解 这些系统在分子水平上的功能可能会导致新的开发 抗生素、阻止生物膜形成和生物膜介导疾病的新方法,以及 阻止转座子介导的抗生素耐药性传播的新方法。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Peggy A Cotter其他文献

The Comparison of Single Serum Antibody Values Against B. pertussis, C. pneumoniae, and M. pneumoniae Antigens in Active Duty American Soldiers in Korea Who Had Prolonged Cough Illnesses (Cases) with Similar Antibody Values in Sera from Well Active Duty Soldiers in Hawaii (Controls)
  • DOI:
    10.1203/00006450-199904020-01051
  • 发表时间:
    1999-04-01
  • 期刊:
  • 影响因子:
    3.100
  • 作者:
    Judy M Vincent;William F Nauschwetz;Andrew Lipton;Craig M Ono;Cory N Costello;Lori Kelsey;Gunther Hsue;Lisa A Jackson;Raffi Tachdjian;Peggy A Cotter;Jeffrey A Gornbein;James D Cherry
  • 通讯作者:
    James D Cherry

Peggy A Cotter的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Peggy A Cotter', 18)}}的其他基金

Cooperative roles of FHA and ACT in Bordetella virulence
FHA 和 ACT 在博德特氏菌毒力中的合作作用
  • 批准号:
    10541178
  • 财政年份:
    2021
  • 资助金额:
    $ 38.88万
  • 项目类别:
Cooperative roles of FHA and ACT in Bordetella virulence
FHA 和 ACT 在博德特氏菌毒力中的合作作用
  • 批准号:
    10206412
  • 财政年份:
    2021
  • 资助金额:
    $ 38.88万
  • 项目类别:
Cooperative roles of FHA and ACT in Bordetella virulence
FHA 和 ACT 在博德特氏菌毒力中的合作作用
  • 批准号:
    10331076
  • 财政年份:
    2021
  • 资助金额:
    $ 38.88万
  • 项目类别:
Contact-dependent signaling and DNA transposition in Burkholderia
伯克霍尔德氏菌中的接触依赖性信号传导和 DNA 转座
  • 批准号:
    10381490
  • 财政年份:
    2020
  • 资助金额:
    $ 38.88万
  • 项目类别:
PlrSR-dependent Signal Transduction in Bordetella Virulence
博德特氏菌毒力中 PlrSR 依赖性信号转导
  • 批准号:
    10097965
  • 财政年份:
    2017
  • 资助金额:
    $ 38.88万
  • 项目类别:
Contact-Dependent Signaling in Burkholderia
伯克霍尔德杆菌中的接触依赖性信号传导
  • 批准号:
    9321389
  • 财政年份:
    2016
  • 资助金额:
    $ 38.88万
  • 项目类别:
Contact-Dependent Signaling in Burkholderia
伯克霍尔德杆菌中的接触依赖性信号传导
  • 批准号:
    9197358
  • 财政年份:
    2016
  • 资助金额:
    $ 38.88万
  • 项目类别:
BcpAIOB-Mediated CDI and Biofilm Formation in Burkholderia pseudomallei
BcpAIOB 介导的鼻疽伯克霍尔德氏菌中的 CDI 和生物膜形成
  • 批准号:
    8750147
  • 财政年份:
    2014
  • 资助金额:
    $ 38.88万
  • 项目类别:
Microbial Pathogenesis: Mechanisms of Infectious Disease
微生物发病机制:传染病的机制
  • 批准号:
    8200143
  • 财政年份:
    2011
  • 资助金额:
    $ 38.88万
  • 项目类别:
Function and Secretion of Bordetella Filamentous Hemagglutinin
博德特氏菌丝状血凝素的功能和分泌
  • 批准号:
    8245274
  • 财政年份:
    2011
  • 资助金额:
    $ 38.88万
  • 项目类别:

相似海外基金

The effects of antibiotics to the transfer frequency of the antibiotic resistance genes and the evolution of high-level resistance.
抗生素对抗生素抗性基因转移频率和高水平抗性进化的影响。
  • 批准号:
    22K05790
  • 财政年份:
    2022
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
NEC05839 Chicken or the Egg: Is AMR in the Environment Driven by Dissemination of Antibiotics or Antibiotic Resistance Genes?
NEC05839 先有鸡还是先有蛋:环境中的抗菌素耐药性是由抗生素或抗生素抗性基因的传播驱动的吗?
  • 批准号:
    NE/N019687/2
  • 财政年份:
    2019
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Research Grant
Combating Antibiotic Resistance to Aminoglycoside Antibiotics through Chemical Synthesis
通过化学合成对抗氨基糖苷类抗生素的耐药性
  • 批准号:
    392481159
  • 财政年份:
    2017
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Research Fellowships
NEC05839 Chicken or the Egg: Is AMR in the Environment Driven by Dissemination of Antibiotics or Antibiotic Resistance Genes?
NEC05839 先有鸡还是先有蛋:环境中的抗菌素耐药性是由抗生素或抗生素抗性基因的传播驱动的吗?
  • 批准号:
    NE/N019687/1
  • 财政年份:
    2016
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Research Grant
Chicken or the Egg: Is AMR in the Environment Driven by Dissemination of Antibiotics or Antibiotic Resistance Genes?
先有鸡还是先有蛋:环境中的抗菌素耐药性是由抗生素或抗生素抗性基因的传播驱动的吗?
  • 批准号:
    NE/N019857/1
  • 财政年份:
    2016
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Research Grant
Chicken or the Egg: Is AMR in the Environment Driven by Dissemination of Antibiotics or Antibiotic Resistance Genes?
先有鸡还是先有蛋:环境中的抗菌素耐药性是由抗生素或抗生素抗性基因的传播驱动的吗?
  • 批准号:
    NE/N019717/1
  • 财政年份:
    2016
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Research Grant
The SuDDICU study- A study of the impact of preventative antibiotics (SDD) on patient outcome and antibiotic resistance in the critically ill in intensive care
SuDDICU 研究 - 一项关于预防性抗生素 (SDD) 对重症监护病危患者的患者预后和抗生素耐药性影响的研究
  • 批准号:
    366555
  • 财政年份:
    2016
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Operating Grants
The SuDDICU study- A study of the impact of preventative antibiotics (SDD) on patient outcome and antibiotic resistance in the critically ill in intensive care
SuDDICU 研究 - 一项关于预防性抗生素 (SDD) 对重症监护病危患者的患者预后和抗生素耐药性影响的研究
  • 批准号:
    361307
  • 财政年份:
    2016
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Operating Grants
Contamination status of antibiotics and antibiotic resistance genes (ARGs) in tropical Asian aquatic environments with artificial and natural disturbance
人工和自然干扰下亚洲热带水生环境中抗生素和抗生素抗性基因(ARG)的污染状况
  • 批准号:
    25257402
  • 财政年份:
    2013
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Grant-in-Aid for Scientific Research (A)
RAPID: COLLABORATIVE RESEARCH: Fate and Transport of Antibiotics and Antibiotic Resistance Genes During Historic Colorado Flood
快速:合作研究:历史性科罗拉多洪水期间抗生素和抗生素抗性基因的命运和运输
  • 批准号:
    1402635
  • 财政年份:
    2013
  • 资助金额:
    $ 38.88万
  • 项目类别:
    Standard Grant
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了