Analytical and Biophysical Research Core

分析和生物物理研究核心

• 批准号: 10611851
• 负责人: Joshua S Sharp
• 金额: $ 34.75万
• 依托单位: UNIVERSITY OF MISSISSIPPI
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-15 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611851
• 关键词: Address; Aerosols; Affinity; Analytical Chemistry; Binding; Bioinformatics; Biological; Biological Assay; Biomedical Research; Biophysics; Carbohydrates; Carbon; Centers of Research Excellence; Characteristics; Charge; Chemistry; Chromatography; Collaborations; Communities; Complex; Complex Mixtures; Computer software; Coupled; Data; Data Analyses; Dedications; Derivation procedure; Detection; Development; Diagnostic; Equipment; Experimental Designs; Fluorescence; Glycobiology; Glycoconjugates; Glycosaminoglycans; Glycosides; Growth; High Pressure Liquid Chromatography; Institution; Link; Mass Spectrum Analysis; Measures; Methods; Mississippi; Modernization; Modification; Natural Products; Nature; Neurosciences; Pathway Analysis; Pathway interactions; Phase; Pilot Projects; Polysaccharides; Porosity; Preparation; Protein-Carbohydrate Interaction; Proteome; Proteomics; Research; Research Personnel; Resolution; Resources; Role; Sampling; Science; Services; Spectrophotometry; Statistical Data Interpretation; Statistical Models; Structure; Structure-Activity Relationship; Support System; Surveys; System; Systems Biology; Techniques; Technology; Testing; Training; Universities; X-Ray Crystallography; absorption; biophysical analysis; biophysical chemistry; biophysical properties; biophysical techniques; chemical release; chemical resource; computational chemistry; data modeling; depolymerization; design; flexibility; instrumentation; interest; investigator training; metabolome; metabolomics; physical property; programs; protein metabolite; secondary metabolite; tandem mass spectrometry; tool

Project Summary/Abstract The University of Mississippi proposes to establish the Glycoscience Center of Research Excellence (GlyCORE), a Phase I Center of Biomedical Research Excellence. Glycoscience is an essential and rapidly emerging field of biomedical science, and the challenging nature of glycoscience research requires tools and expertise that are not commonly found in the biomedical research community. Moreover, the biomedical community is rapidly growing to appreciate the essential role of carbohydrates, with researchers outside of the self-identified “glycoscience” community finding themselves faced with addressing challenging questions in glycoscience as part of the course of their biomedical researchers. The focus of the Analytical and Biophysical Research Core is three-fold: to train investigators new to glycoscience in proper carbohydrate sample preparation and handling; to support hypothesis-driven research with targeted quantitative analyses from both enriched samples and complex mixtures; and to support discovery-based research to give investigators a system-level view of changes upon perturbation of targeted systems. Training of researchers is a key function of the Core, especially in Phase I when the institutional level of expertise in glycoscience is in its earliest stage of development. Core 1 will train researchers in carbohydrate and carbohydrate conjugate sample preparation techniques. Targeted analyses will be provided as a key technology for hypothesis-driven research, where an investigator wants to test for the presence and quantity of a given glycan or glycoside, structurally characterize an unknown molecule, or validate a key biological or chemical resource. Structural analyses of glycan modification, protein-carbohydrate interactions, binding affinity estimations, and other biophysical analyses will be supported through MS-, LC- and spectrophotometry-based technologies. Access to NMR instrumentation and expertise in spectral acquisition and interpretation will also be provided. Finally, Core 1 will offer “omics”-style discovery-based surveys of glycomes, proteomes, metabolomes, etc. in quantitative, semi-quantitative, and qualitative fashions, supporting systems glycobiology and hypothesis development for targeted assay design. Core 1 will interface closely with the Computational Chemistry and Bioinformatics Core to provide advanced statistical and pathway analyses for “omics” data. The services of Core 1 will use both existing University of Mississippi resources as well as a dedicated high resolution Thermo Q-Exactive Plus LC-MS/MS system and a Dionex Ultimate 3000 analytical UHPLC system. Due to the poor UV-absorption characteristics of most carbohydrates, fluorescence detection for tagged carbohydrates and charged aerosol detection of underivatized carbohydrates will also be available for sensitive detection and quantitation at high dynamic range. By focusing our Phase I Analytical and Biophysical Core on a small number of flexible technologies, we can support the diverse glycoscience interests of UM investigators with cutting-edge technology using a limited array of equipment and have room for growth as GlyCORE matures.

项目总结/摘要 密西西比大学提议建立糖科学卓越研究中心 （GlyCORE），生物医学研究卓越的第一阶段中心。糖科学是一个必不可少的和快速的 生物医学科学的新兴领域，以及糖科学研究的挑战性，需要工具和 生物医学研究界不常见的专业知识。此外，生物医学 社区正在迅速发展，以了解碳水化合物的重要作用，与研究人员以外的 自我认定的“糖科学”社区发现自己面临着解决具有挑战性的问题， 将糖科学作为其生物医学研究人员课程的一部分。分析和生物物理学的重点 研究核心有三个方面：在适当的碳水化合物样品中培训新的糖科学研究人员 准备和处理;通过有针对性的定量分析支持假设驱动的研究， 富集样品和复杂混合物;并支持基于发现的研究， 在目标系统扰动时的变化的系统级视图。培训研究人员是关键 核心的功能，特别是在第一阶段，当糖科学的专业知识的机构水平是在其 发展的最早阶段。核心1将培训研究人员在碳水化合物和碳水化合物共轭 样品制备技术将提供有针对性的分析，作为假设驱动的关键技术 研究，其中研究者想要测试给定聚糖或糖苷的存在和数量， 从结构上表征未知分子，或验证关键生物或化学资源。结构 分析聚糖修饰、蛋白质-碳水化合物相互作用、结合亲和力估计和其他 生物物理分析将通过质谱、液相色谱和质谱技术得到支持。接入 还将提供核磁共振仪器和光谱采集和解释方面的专业知识。最后， 核心1将提供“组学”风格的发现为基础的调查，糖，蛋白质组，代谢组等， 定量、半定量和定性方式，支持系统糖生物学和假设 用于靶向测定设计的开发。核心1将与计算化学紧密结合， Bioinformatics Core为“组学”数据提供先进的统计和途径分析。的服务 核心1将使用现有的密西西比大学资源以及专用的高分辨率Thermo Q-Exactive Plus LC-MS/MS系统和Dionex Ultimate 3000分析型UHPLC系统。由于穷人的 大多数碳水化合物的紫外吸收特性，标记碳水化合物的荧光检测， 未衍生碳水化合物的带电气溶胶检测也将用于灵敏检测， 高动态范围下的定量。通过将我们的第一阶段分析和生物物理核心集中在一个小的 凭借多种灵活的技术，我们可以支持UM研究人员的各种糖科学兴趣， GlyCORE使用有限的设备，拥有尖端技术，并随着GlyCORE的成熟而有增长空间。