Hydrogen Sulfide Regulation in Cardioprotection

项目摘要 硫化氢是一种重要的信号分子，具有很强的细胞保护作用 心血管系统中的作用。目前的结果表明，硫化氢水平的调制 可能对血管扩张等多种疾病状态有潜在的治疗价值， 抗炎、抗氧化。然而，确切的作用机制仍然是 不清楚以及显示最佳保护所需的实际硫化氢浓度/流量 这些疾病模型还有待确定。这种知识的缺乏是一个主要的负担 在以硫化氢为基础的治疗的发展中。为了解决这个问题，研究工具可以 需要有效地检测生物样品中的硫化氢，特别是提供/去除生物样品中的硫化氢。 在这一应用中，我们计划开发用于检测硫化氢的高灵敏度荧光探针。 我们还将开发新型的硫化氢释放剂和清除剂。新的 将使用化学品来确定模型中用于心脏保护的最佳硫化氢配置文件 心肌缺血再灌注(MI/R)损伤与心力衰竭。这项研究将确立 指导硫化氢治疗发展的有用方案。

Project Summary Hydrogen sulfide (H2S) is an important signaling molecule with potent cytoprotective actions in cardiovascular systems. Current results indicate that modulation of H2S levels could have potential therapeutic value for a number of disease states such as vasodilation, anti-inflammation, and anti-oxidation. However, the exact mechanisms of action are still unclear and the actual H2S concentration/flux needed to exhibit the optimal protection in those disease models are to be determined. This lack of knowledge presents a major burden in the development of H2S based therapy. To solve this problem, research tools that can effectively detect H2S and specifically supply/remove H2S in biological samples are needed. In this application we plan to develop highly sensitive fluorescent probes for H2S detection. We will also develop novel H2S releasing agents and scavenging agents. The new chemicals will be used to determine the optimal H2S profiles for cardioprotection in models of myocardial ischemia/reperfusion (MI/R) injury and heart failure. This study will establish useful protocols guiding the development of H2S therapy.