Hydrogen Sulfide Regulation in Cardioprotection

项目摘要 硫化氢（H2S）是一种重要的信号分子，具有强的细胞保护作用 对心血管系统的作用。目前的结果表明，H2S水平的调节 可能对许多疾病状态具有潜在的治疗价值， 抗炎、抗氧化。然而，确切的作用机制仍然是 不清楚和实际的H2S浓度/流量需要表现出最佳的保护， 这些疾病模型有待确定。这种知识的缺乏是一个主要的负担 H2S疗法的发展。为了解决这个问题，研究可以 需要有效地检测H2S并特别地提供/除去生物样品中H2S。 在此应用中，我们计划开发用于H2S检测的高灵敏度荧光探针。 我们还将开发新型H2S释放剂和清除剂。新 化学品将用于确定最佳的H2S配置文件的心脏保护模型， 心肌缺血/再灌注（MI/R）损伤和心力衰竭。这项研究将建立 指导H2S治疗发展的有用协议。

Project Summary Hydrogen sulfide (H2S) is an important signaling molecule with potent cytoprotective actions in cardiovascular systems. Current results indicate that modulation of H2S levels could have potential therapeutic value for a number of disease states such as vasodilation, anti-inflammation, and anti-oxidation. However, the exact mechanisms of action are still unclear and the actual H2S concentration/flux needed to exhibit the optimal protection in those disease models are to be determined. This lack of knowledge presents a major burden in the development of H2S based therapy. To solve this problem, research tools that can effectively detect H2S and specifically supply/remove H2S in biological samples are needed. In this application we plan to develop highly sensitive fluorescent probes for H2S detection. We will also develop novel H2S releasing agents and scavenging agents. The new chemicals will be used to determine the optimal H2S profiles for cardioprotection in models of myocardial ischemia/reperfusion (MI/R) injury and heart failure. This study will establish useful protocols guiding the development of H2S therapy.