Stony Brook University Laboratory for Comparative Medicine to Support Pandemic Preparedness

石溪大学比较医学实验室支持流行病防范

• 批准号: 10611662
• 负责人: David G Thanassi
• 金额: $ 293.31万
• 依托单位: STATE UNIVERSITY NEW YORK STONY BROOK
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611662
• 关键词: 2019-nCoV; Adenoviruses; Aerosols; American; Animal Model; Animals; Applied Research; Area; Basic Science; Biological Products; Biotechnology; Bunyavirales; COVID-19; COVID-19 therapeutics; COVID-19 vaccine; Containment; Coronavirus; Custom; Dengue Virus; Detection; Disease; Duct (organ) structure; Faculty; Family; Family Picornaviridae; Flaviviridae; Francisella tularensis; Funding; Future; Gases; HIV; Hantavirus; High temperature of physical object; Immune response; Immunotherapy; Individual; Infection; Influenza A virus; Investigation; Laboratories; Measures; Medicine; Mycobacterium tuberculosis; New York; Pathogenesis; Persons; Phase; Play; Plumbing; Population; Powassan virus; Preventive; Process; Publications; Quantitative Reverse Transcriptase PCR; RNA Viruses; RNA analysis; Recording of previous events; Research; Research Activity; Research Personnel; Resources; Reverse Transcription; Rickettsia; Rodent; Role; Security; Steam; System; Therapeutic; Time; Translational Research; Treatment Efficacy; Tuberculosis; Tularemia; Universities; Viral; Virulent; Virus; Water; Work; Zika Virus; Zoonoses; animal imaging; anti-viral efficacy; antimicrobial; antiviral drug development; biosafety level 3 facility; combat; comparative; cost; design; emerging pathogen; equipment acquisition; exhaust; experience; flexibility; fluorescence activated cell sorter device; heat exchanger; improved; infectious disease model; interest; metropolitan; microbial; operation; pandemic disease; pandemic preparedness; pathogen; pathogenic bacteria; pathogenic fungus; pathogenic microbe; pathogenic virus; pre-clinical; pre-clinical research; programs; spotted fever; tool; virology

PROJECT SUMMARY/ABSTRACT The Laboratory for Comparative Medicine (LCM) at Stony Brook University is a recently constructed, state-of- the-art animal biosafety level 3 (ABSL3) facility. The LCM is actively engaged in basic, translational, and preclinical research on SARS-CoV-2, the viral agent of the current COVID-19 worldwide pandemic. Research in the LCM serves multiple individual investigators and groups, both within and outside of Stony Brook University, and includes commercial as well as academic interests. Stony Brook has a long history of excellence in microbial pathogenesis research, including studies on pandemic potential viruses of concern in the families Coronaviridae (SARS-CoV-2), Bunyavirales (Hantaviruses), Flaviviridae (Dengue, Zika, and Powassan viruses), and Picornaviridae (Poliovirus). Stony Brook virologists are also conducting ongoing studies in Human Immunodeficiency Virus and Influenza A Virus. Our research over the past five years has covered three different RNA virus families relevant under the American Pandemic Preparedness/pandemic preparedness effort, and our cumulative experience covers four of the viral families of concern. In addition to viruses of concern, active research at Stony Brook and in the LCM includes work on the highly virulent bacterial agents Rickettsia spp. of the spotted fever group, Francisella tularensis, a Select Agent and cause of the zoonotic disease tularemia, and Mycobacterium tuberculosis, causative agent of tuberculosis. To improve facility operations and enhance ongoing and future research in the LCM, we are requesting four upgrades to the LCM: 1) Replacement of the high temperature hot water unfired steam boilers and conversion of the high temperature and hot water systems; 2) Purchase and installation of a washer for animal cages and racks; 3) Purchase and installation of a fluorescence-activated cell sorter and custom biosafety enclosure; and 4) Acquisition of a real-time, quantitative reverse-transcription PCR machine. The requested upgrades will support and improve pandemic preparedness-relevant research in the LCM, advance studies on highly virulent and emerging pathogens, and provide enhanced resources not only to Stony Brook researchers, but also to State and regional efforts to combat current and future pandemic threats. The requested improvements will support the development of antiviral programs and antimicrobial preventive and therapeutic measures.

项目总结/摘要 斯托尼布鲁克大学的比较医学实验室（LCM）是一个最近建成的， 动物生物安全3级（ABSL 3）设施。LCM积极参与基础，翻译， SARS-CoV-2的临床前研究，SARS-CoV-2是当前COVID-19全球大流行的病毒因子。研究 LCM为斯托尼布鲁克内外的多个调查人员和小组提供服务 大学，包括商业和学术利益。斯托尼布鲁克历史悠久， 在微生物致病机理研究方面的卓越成就，包括对 冠状病毒科（SARS-CoV-2）、布尼亚病毒目（汉坦病毒）、黄病毒科（登革热、寨卡病毒和 Powassan病毒）和小核糖核酸病毒科（脊髓灰质炎病毒）。斯托尼布鲁克的病毒学家也正在进行 人类免疫缺陷病毒和甲型流感病毒的研究。我们过去五年的研究 涵盖了与美国大流行防范/大流行相关的三种不同的RNA病毒家族 准备工作，我们积累的经验涵盖了四个令人关切的病毒家族。除了 关注的病毒，积极研究在斯托尼布鲁克和在LCM包括工作的高毒力 细菌性病原体立克次体属土拉热弗朗西丝氏菌，一种选择性病原体， 人畜共患病兔热病和结核病病原体结核分枝杆菌。提高 设施的运作和加强正在进行的和未来的研究在LCM，我们要求四个升级， LCM：1）更换高温热水不燃蒸汽锅炉，改造高温热水锅炉， 温度和热水系统; 2）购买和安装动物笼架清洗机; 3） 购买和安装荧光激活细胞分选仪和定制生物安全外壳;以及 获得实时定量逆转录PCR仪。所要求的升级将 支持和改进LCM中与大流行病相关的研究，推进高毒力 和新出现的病原体，并提供增强的资源，不仅斯托尼布鲁克的研究人员， 国家和区域努力应对当前和未来的流行病威胁。所要求的改进将 支持制定抗病毒方案和抗菌预防和治疗措施。