Improving The Scripps Research Institute BSL3 Capabilities to Combat Viruses of Pandemic Concern

提高斯克里普斯研究所 BSL3 对抗流行病病毒的能力

• 批准号: 10611798
• 负责人: Juan C. de la Torre
• 金额: $ 386.96万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10611798
• 关键词: 18 year old; Address; Aging; Air; Air Movements; Animals; Applications Grants; Area; Bunyavirales; COVID-19 pandemic; Cell Separation; Cells; Chromium; Climate; Color; Computer software; Copper; Coronavirus; Data; Devices; Duct (organ) structure; Engineering; Ensure; Equipment; Facility Construction; Faculty; Failure; Filovirus; Flaviviridae; Frequencies; Funding; Future; Gene Expression; Goals; Hamsters; Heating; Housing; Image; Immune; Immunotherapeutic agent; Infrastructure; Leadership; Life Expectancy; Light; Mechanics; Mission; Modernization; Molecular Biology; Mus; Pharmacologic Substance; Procedures; RNA Viruses; Research; Research Activity; Research Institute; Research Personnel; Research Project Grants; Research Support; Resources; Safety; Soil; System; Therapeutic; Time; Togaviridae; Update; Virus; Work; antiviral drug development; biosafety level 3 facility; combat; cost; cost effective; design; digital; exhaust; fluorescence microscope; high efficiency particulate air filter; imaging system; improved; operation; pandemic disease; pandemic preparedness; pressure; programs; rate of change; sensor; single cell analysis; trend; vibration

Project Summary/Abstract The goal of the application “Improving The Scripps Research Institute BSL3 Capabilities To Combat Viruses Of Pandemic Concern” is to renovate and upgrade The Scripps Research Institute (TSRI) current ABSL3 and BSL3 facilities. This will enhance TSRI research capabilities to combat RNA viruses of pandemic potential. TSRI has ~ 2,000 sf dedicated to ABSL3 and ~ 1,500 sf dedicated to BSL3. Currently, these facilities support research projects from eight TSRI faculty whose combined areas of expertise cover research on RNA viruses of pandemic potential, including Bunyavirales, Coronaviridae, Flaviviridae, Filoviridae and Togaviridae. In addition, the facilities are supporting several projects sponsored by pharmaceutical companies. The specific studies have strong translational components aimed at developing antiviral drugs and immunotherapeutics against these RNA viruses. TSRI ABSL3 and BSL3 suites, located in the Molecular Biology Building (MBB), have been operating 24/7 since the last renovations in 2004. The existing mechanical systems have been maintained beyond ASHREA equipment life expectancy for the La Jolla, Ca. coastal climate. In addition, the original design from 1985-87 has resulted in underutilization of ~160 sf in the ABSL3 and ~400 sf in the BSL3. To correct these deficiencies, TSRI has completed a conceptual design with FPBA Architects & tk1sc Mechanical Engineers. This plan includes replacing the aging HVAC systems of the ABSL3 and BSL3 space to increase airflow capacity with N+1 redundancy and update the HEPA filtration system of the ABSL3. In addition, the project will modernize the biocontainment suites by increasing holding capacity (ABSL3), creating a select agent isolation room (BSL3), and installing a new sterilizer (ABSL3). The current ABSL3/BSL3 facilities lack imaging, cell sorting, and single cell analysis capabilities. To remedy these deficiencies, we propose adding to the facility the EVOSTM Imaging System, the SH800S Cell Sorter and the Chromium iX system. These improvements will help to address the increasing demands by TSRI faculty for ABSL3/BSL3 space, time and improved functional capabilities, while ensuring the highest level of safety during daily operations of the facility. Moreover, research activities proposed under the Antiviral Program for Pandemics (APP) U19AI171443 AViDD Grant Application, will greatly benefit from the proposed improvements to the current TSRI ABSL3 and BSL3 facilities. The project will be managed by a well-integrated and highly qualified team, including leaders from Facilities Construction and Engineering, the Institutional Animal Program and Resources, EHS and Biosafety, the Executive VP Office, and investigators directly involved in ABSL3/BSL3 research activities. In addition, the application has the unwavering support of the TSRI leadership.

项目总结/摘要 该应用程序的目标是“提高斯克里普斯研究所BSL 3的能力，以打击病毒的 大流行关注”是改造和升级斯克里普斯研究所（TSRI）目前的ABSL 3和BSL 3 设施这将增强TSRI的研究能力，以对抗具有大流行潜力的RNA病毒。TSRI拥有 ABSL 3专用约2，000 sf，BSL 3专用约1，500 sf。目前，这些设施支持研究 来自TSRI八名教师的项目，他们的专业领域包括大流行RNA病毒的研究 潜在的，包括布尼亚病毒目，冠状病毒科，黄病毒科，丝状病毒科和披膜病毒科。此外该 这些设施正在支持制药公司赞助的几个项目。具体的研究有 旨在开发针对这些RNA的抗病毒药物和免疫治疗剂的强翻译组分 病毒位于分子生物学大楼（MBB）的TSRI ABSL 3和BSL 3套房已开始运行 自2004年最后一次装修以来，24/7。现有的机械系统已被维护， ASHREA设备预期寿命的拉霍亚，加利福尼亚州。沿海气候此外，原设计从 1985- 1987导致ABSL 3中约160 sf和BSL 3中约400 sf的利用不足。地纠正了这些 TSRI与FPBA建筑师和tk 1 sc机械工程师完成了概念设计。这 计划包括更换ABSL 3和BSL 3空间的老化HVAC系统，以增加气流容量， N+1冗余并更新ABSL 3的HEPA过滤系统。此外，该项目还将使 通过增加容纳能力（ABSL 3），创建选择剂隔离室（BSL 3）， 并安装新的灭菌器（ABSL 3）。目前的ABSL 3/BSL 3设施缺乏成像、细胞分选和单克隆抗体。 细胞分析能力。为了弥补这些不足，我们建议增加EVOSTM成像设备 系统、SH 800 S细胞分选仪和Chromium iX系统。这些改进将有助于解决 TSRI教员对ABSL 3/BSL 3空间、时间和改进功能能力的需求不断增加， 确保设施日常运营期间的最高安全水平。此外，拟议的研究活动 根据流行病抗病毒计划（APP）U19 AI 171443 AViDD赠款申请，将大大受益 从目前TSRI ABSL 3和BSL 3设施的拟议改进。该项目将由 由一个高度整合和高素质的团队，包括设施建设和工程的领导， 机构动物计划和资源、EHS和生物安全、执行副总裁办公室和研究人员 直接参与ABSL 3/BSL 3研究活动。此外，该应用程序还得到以下方面的坚定支持： TSRI领导层。