Reducing Infection and Plugging of Tympanostomy Tubes Through Zwitterionic Thin Films
通过两性离子薄膜减少鼓膜造口管的感染和堵塞
基本信息
- 批准号:10615107
- 负责人:
- 金额:$ 5.27万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2021
- 资助国家:美国
- 起止时间:2021-05-26 至 2024-05-25
- 项目状态:已结题
- 来源:
- 关键词:AdhesionsAffectBacteriaBacterial AdhesionBiocompatible MaterialsBlood PlateletsChildComplexConductive hearing lossDevicesDrynessEarEngineeringEustachian TubeExposure toFamily suidaeFibrinogenFibroblastsFilmFluorescenceGoalsGreen Fluorescent ProteinsHealthHydration statusHydrogelsImplantIn VitroInfectionIntestinesLegal patentLongevityMacrophageMeasuresMethacrylatesMethodsMicrobial BiofilmsMicroscopyMissionModificationMucous body substanceNational Institute on Deafness and Other Communication DisordersObstructionOperative Surgical ProceduresOtitis MediaOtorrheaOutcomePeriodic acid Schiff stain methodPhysiciansPolyethylene GlycolsPolymersProcessPropertyPseudomonas aeruginosaPublic HealthRattusReaderRecurrenceResearchResistanceScientistStainsStandardizationStaphylococcus aureusStaphylococcus epidermidisSurfaceTechnologyTestingThinnessTrainingTranslational ResearchTubeTympanic membraneTympanometryTympanostomyUnited States National Institutes of Healthcrosslinkdensityeffectiveness evaluationexperimental studyfunctional groupimprovedin vitro Modelinnovationmonomernovelpatient subsetspolymerizationpreventsurface coating
项目摘要
PROJECT SUMMARY
Although tympanostomy tube (TT or ear tube) placement is a simple and common surgery (754,000/year in the
US), complications arise in a subset of patients, particularly post-TT otorrhea (PTTO, 26%) due to bacterial
biofilms and mucus plugging (7-34%). There is an urgent need to reduce these complications and enhance the
functional longevity of TTs. Modification of TT surfaces to prevent bacterial adhesion and to reduce mucus
adhesion could dramatically reduce these complications. We recently developed a novel photochemically
driven process that simultaneously forms and grafts zwitterionic thin films onto polymeric materials used to
fabricate TTs (e.g. PDMS). These thin films are polymerized from either sulfobetaine methacrylate (SBMA) or
carboxybetaine methacrylate (CBMA) monomers. The CBMA/SBMA polymer chains are crosslinked to each
other during polymerization by polyethylene glycol dimethacrylate (PEGDMA). We find that both CBMA and
SBMA thin films prevent adhesion by an order of magnitude from fibrinogen, platelets, macrophages,
fibroblasts, and importantly, Staphylococcus aureus and Staphylococcus epidermidis. We propose to extend
these promising findings into TTs, while determining how changes in crosslinking density affect end properties.
The overall objective of this research is to determine the ability of zwitterionic thin films grafted onto the surface
of TTs to prevent the complex phenomena of bacterial colonization and mucus plugging. We hypothesize that
CBMA and SBMA zwitterionic thin films will prevent bacterial adhesion and reduce mucus plugging on TTs. We
will test this hypothesis by undertaking the following Specific Aims.
1) Determine the effect of CBMA and SBMA thin films on bacteria adhesion from Staphylococcus aureus and
Pseudomonas aeruginosa on TTs. We will test this effect by quantifying bacterial adhesion on PDMS surfaces
using validated in vitro models. We will also expose rats that have been implanted with zwitterionic coated or
bare TTs with either S. aureus or P aeruginosa to induce biofilm formation.
2) Investigate the ability of CBMA and SBMA thin films to reduce mucus adhesion, mucus drying, and mucus
plug formation on TT surfaces. To test this, we will first examine the adhesion and drying of porcine intestinal
mucus on PDMS that have been coated with thin films or remain uncoated. Finally, we will test the mucus
plugging on coated and uncoated TTs inserted into rat tympanic membranes.
When successful, these aims will implicate a novel and highly effective method to prevent common
complications of TTs by preventing biofilm and mucus plug formation. Beyond TTs, the results will be broadly
applicable to devices which suffer from biofouling. This innovation will reduce the PTTO complications currently
suffered by ~50,000 US children per year and thus support the Mission, Goals, and Objectives of the NIDCD.
项目总结
项目成果
期刊论文数量(0)
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科研奖励数量(0)
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Ryan Horne其他文献
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{{ truncateString('Ryan Horne', 18)}}的其他基金
Reducing Infection and Plugging of Tympanostomy Tubes Through Zwitterionic Thin Films
通过两性离子薄膜减少鼓膜造口管的感染和堵塞
- 批准号:
10321467 - 财政年份:2021
- 资助金额:
$ 5.27万 - 项目类别:
Reducing Infection and Plugging of Tympanostomy Tubes Through Zwitterionic Thin Films
通过两性离子薄膜减少鼓膜造口管的感染和堵塞
- 批准号:
10412978 - 财政年份:2021
- 资助金额:
$ 5.27万 - 项目类别:
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