Single Cell and Immunoanalysis Core

项目摘要/摘要：单细胞和免疫分析核心 由于缺乏转基因技术，人类皮肤生物学和炎症性皮肤病的研究进展缓慢。 模型系统和可以从活着的患者身上获得的有限样本。然而，高级分析 从小样本中提供最大信息的技术已经开发出来，具有潜在的 以增进我们对人类皮肤病的了解。这一核心使关键的开创性技术 包括单细胞RNA测序(scRNA-seq)，具有批量样本、发现TCR基因和 抗体标记细胞(CITE-SEQ)，以及单细胞ATAC-SEQ，通过流式细胞仪进行单细胞成像， 研究人员可通过飞行时间(CyTOF)和高通量T细胞受体测序获得 任何希望开展人类皮肤病研究的机构。该中心还提供以下方面的援助 每项技术的实验计划、关键试剂和协助数据分析。例如， 中心提供的协议允许皮肤活组织检查从远程位置运送并可见地冷冻保存 在scrna-seq研究之前；这一进步使研究人员可以使用单细胞图谱来研究样本。 来自临床试验和未被充分研究的皮肤病。该中心还提供细胞TOF染色方案， 细胞因子抗体的验证面板，细胞因子抗体的定制结合，对实验的建议 设计和数据分析，以及外部调查人员将其细胞送到中心进行研究所需的所有服务 细胞飞行时间研究。该中心在实验设计、DNA提取等方面提供全面支持 协议、DNA样本准备服务、运输和数据分析协助，将使 研究人员成功地利用高通量TCR测序来分析皮肤免疫反应和 跨多个时间点和组织跟踪致病T细胞克隆。我们收录了36个研究项目 来自希望利用本核心服务的调查人员。总而言之，该核心提供了对单个 有可能为人类皮肤生物学提供前所未有的洞察的细胞方法 疾病。

Project Summary/Abstract: The Single Cell and Immunoanalysis Core Research into human skin biology and inflammatory skin diseases has been slowed by the lack of transgenic model systems and the limited samples that can be obtained from living patients. However, advanced analytic techniques that provide maximal information from small samples have been developed that have the potential to advance our understanding of human skin disease. This Core makes critical groundbreaking techniques including single cell RNA sequencing (scRNA-seq) with the ability to batch samples, discover TCR genes and tag cells with antibodies (CITE-seq), as well as single cell ATAC-seq, single cell imaging by flow cytometry, cytometry by time of flight (CyTOF) and high throughput T cell receptor sequencing available to investigators at any institution who wish to carry out human skin disease research. The Center also provides assistance with experimental planning, key reagents and assistance with data analysis for each technique. For example, the Center provide protocols that allow skin biopsies to be shipped from distant locations and viably cryopreserved before scRNA-seq studies; this advance makes single cell profiling available to investigators to study samples from clinical trials and from understudied skin diseases. The Center also provides CyTOF staining protocols, validated panels of CyTOF antibodies, custom conjugation of CyTOF antibodies, advice on experimental design and data analysis, and all services needed for outside investigators to send their cells to the Center for CyTOF studies. The Center provides comprehensive support in the experimental design, DNA extraction protocols, DNA sample preparation services, shipping, and data analysis assistance that will enable researchers to successfully utilize high throughput TCR sequencing to profile skin immune responses and track pathogenic T cell clones across multiple timepoints and tissues. We have included 36 research projects from investigators who wish to utilize the services of this Core. In summary, this core provides access to single cell approaches that have the potential to provide unprecedented insights into the biology of human skin disease.