Consumption of Contaminated Passaic River Fish Causes Gut Microbiome Dysbiosis and May Influence Metabolic Syndrome Outcomes

食用受污染的帕塞克河鱼会导致肠道微生物群失调，并可能影响代谢综合征的结果

• 批准号: 10614069
• 负责人: Seung Gee Kim
• 金额: $ 4.13万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10614069
• 关键词: Address; Adult; Affect; Area; Awareness; Biological; Blood Pressure; Characteristics; Communities; Community Health; Consumption; Crabs; Data; Diabetes Mellitus; Diet; Dioxins; Disease; Disease susceptibility; Dose; Eels; Elderly; Endocrine; Environment; Exposure to; Family; Feces; Fishes; Food; Gastrointestinal tract structure; Germ-Free; Goals; Grant; Health; Health Policy; Health protection; Healthcare; High Density Lipoproteins; Home; Hypertension; Immunity; Individual; Intervention; Life; Link; Lipids; Low Income Population; Measures; Mediating; Mercury; Metabolic; Metabolic Diseases; Metabolic syndrome; Metabolism; Modeling; Mus; Outcome; Paracrine Communication; Pathway interactions; Phenotype; Pilot Projects; Play; Policy Maker; Pollution; Polychlorinated Biphenyls; Population; Predisposition; Pregnant Women; Principal Component Analysis; Probiotics; Production; Public Health; Publishing; Recommendation; Research; Ribosomal RNA; Role; Sampling; Schedule; Serum; Signal Transduction; Source; Structure; Surveys; Testing; Tetrachlorodibenzodioxin; Tissues; Translating; Triglycerides; Vulnerable Populations; bacterial community; contaminated fish; dietary control; dysbiosis; efficacious treatment; exposed human population; fasting glucose; feeding; gut dysbiosis; gut microbiome; gut microbiota; immunoregulation; improved; lipid metabolism; male; marine; member; metabolic phenotype; microbiome analysis; microbiota; pollutant; poor communities; pregnant; prevent; probiotic therapy; remediation; response; sex; superfund site; training opportunity; translational potential

The Newark Bay/Passaic River region is home to a Superfund site that is heavily polluted with PCBs, mercury, and record levels of dioxin. There is very strong evidence that the marine life inhabiting these polluted waterways are contaminated with these toxic agents. Bottom or filter feeders, along with predatory species, often accumulate significant amounts of contaminants in their body tissue. Consumption of these species by humans exposes them to high levels of contaminants, which can lead to adverse health effects. Despite these findings and posted public warnings that vulnerable populations (e.g., pregnant women, babies, or elderly) should not consume marine life captured in these waterways, exposure through consumption continues in poor communities. A pilot study done by our lab sequenced 16S rRNA from the feces of adult mice that were fed autoclaved diets supplemented with 2.5% or 7.5% “dirty” Passaic River fish. A principal component analysis (PCA) demonstrated that consumption of “contaminated” eel feed sourced from the Passaic River affected GI tract community diversity and structure in male mice at both days 1 and 9, compared to the “clean” feed group. Because the gut microbiome encompasses key metabolic and life functions, such as endocrine signaling, lipid production, metabolism, and immune regulation, dysbiosis, or an imbalance of, bacterial community structure can lead to significant changes in overall wellness. Phenotypes may include those of metabolic syndrome - This grant aims to study how GI tract dysbiosis from consumption of contaminated Passaic River fish contributes to the biological effects of metabolic syndrome, which will be characterized as hypertension and triglyceride, HDL-C, and fasting glucose levels. We propose to do this by observing shifts in community structure of the gut microbiome, and analyzing phenotypes of metabolic disease in mice that have consumed contaminated fish captured from this waterway. Importantly, we will focus on mixtures and synergistic effects of contaminants, as it is unusual for such contaminants to exist independently of others. This research will model both environmentally relevant concentrations and real-world exposure scenarios where contaminated fish are consumed by anglers and their families. Finally, we will explore the preventative effects of probiotic treatment on GI tract dysbiosis. These findings will translate into healthier communities by raising awareness, reducing disease susceptibility, and protecting the health of poorer communities where families regularly rely on caught fish as sustenance. This project will provide valuable training opportunities in environmentally relevant coexposure assessments, microbiome analyses, and community health awareness as data is translated into public health policy and disease mitigation strategies.

纽瓦克湾（Newark Ba​​y）/Passaic河地区是超级基金遗址的所在地，该地点被PCB，汞和创二恶英水平污染。有非常有力的证据表明，居住在这些污染水道的海洋生物被这些有毒药物污染。底部或过滤器进料器以及掠食性物种通常会在其身体组织中积累大量的污染物。人类对这些物种的消费使它们暴露于高水平的污染物中，这可能导致不利的健康影响。尽管有这些发现并发布了公开警告，该警告称脆弱的人口（例如孕妇，婴儿或年龄较大）不应在这些水道中捕获的海洋生物，但贫困社区的消费量仍在继续。我们的实验室测序的一项试点研究是从成年小鼠粪便中进行的16S rRNA，这些小鼠被喂食，这些小鼠被喂食，并补充了2.5％或7.5％的“脏” Passaic河鱼。主要成分分析（PCA）表明，与“清洁”的饲料组相比，在两天和第9天，从Passaic River产生的“受污染”的鳗鱼饲料影响了雄性小鼠的胃肠道群落多样性和结构。因为肠道微生物组包括关键的代谢和生命功能，例如内分泌信号传导，脂质产生，代谢和免疫学，营养不良或失衡，细菌群落结构可能会导致整体健康的重大变化。表型可能包括代谢综合征的表型 - 该赠款旨在研究胃肠道疾病的胃肠道疾病的消耗如何促进代谢综合征的生物学作用，该作用将以高血压和甘油三酸酯，HDL -C，HDL -C和禁食糖水平为特征。我们建议通过观察肠道微生物组的社区结构的变化来做到这一点，并分析食用从该水道捕获的被污染的鱼类的小鼠中的代谢疾病表型。重要的是，我们将重点关注污染物的混合物和协同作用，因为这种污染物独立于他人存在是不寻常的。这项研究将模拟与环境相关的浓度和现实接触场景，在这些情况下，垂钓者及其家人消耗了受污染的鱼类。最后，我们将探讨益生菌治疗对胃肠道营养不良的预防作用。这些发现将通过提高认识，降低疾病的易感性并保护家庭经常依靠捕捞鱼作为寄生的贫困社区的健康来转化为更健康的社区。随着数据被转化为公共卫生政策和缓解疾病的策略，该项目将在环境相关的共曝光评估，微生物组分析和社区健康意识中提供宝贵的培训机会。