CORE B Symington

核心 B 赛明顿

• 批准号: 10614994
• 负责人: Lorraine S Symington
• 金额: $ 12.22万
• 依托单位: COLUMBIA UNIVERSITY HEALTH SCIENCES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-08 至 2025-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10614994
• 关键词: Biological Assay; Biological Markers; Cell Line; Cells; Chromosomal Breaks; Chromosomal translocation; Chromosome abnormality; Clonal Expansion; Complement; Custom; Cytogenetic Analysis; Cytogenetics; DNA; DNA Damage; DNA Double Strand Break; DNA Repair; DNA Repair Pathway; DNA Sequence Rearrangement; DNA sequencing; Data Analyses; Dedications; Double Strand Break Repair; Ensure; Excision; Experimental Designs; G-Banding; Gamma-H2AX; Genomic Instability; Genomic approach; Human; Image; Karyotype determination procedure; Leadership; Length; Malignant Neoplasms; Mammalian Cell; Measures; Methods; Molecular Analysis; Molecular Cytogenetics; Paint; Primary Neoplasm; Productivity; Recurrence; Repetitive Sequence; Research Personnel; Ribosomal DNA; Services; Single-Stranded DNA; Site; Spectral Karyotyping; Topoisomerase Inhibitors; Validation; Yeasts; clastogen; cost effectiveness; data acquisition; detection assay; experience; irradiation; member; p53-binding protein 1; programs; repaired; response; single molecule; telomere; tool

SUMMARY The Molecular Analysis of Genomic Instability Core will perform state-of-the-art assays for program investigators in the analyses of cytogenetic aberrations, and DNA damage responses and repair. These analyses will complement sequencing results (e.g., examine repetitive regions that are not covered by DNA sequencing approaches), and provide experimental validation for recurrent genomic instability and rearrangements identified using genomic approaches (Core C). In addition, the core will perform cell-based assays to detect and quantify biomarkers for DNA damage and DNA repair pathways, and measure DNA repair intermediates. Having a dedicated core for this program is beneficial: 1) for cost effectiveness and 2) for custom designing the experiments based on specific requirement for each of the projects. By centralizing these services under the leadership of two experienced investigators, Drs. Lorraine Symington and Shan Zha, the Core will enhance the productivity of the program project members by providing essential services to all projects, and ensure uniformity of data acquisition and interpretation. The core has two independent specific aims, which are built upon the expertise of the core leaders. The purpose of Aim 1 is to develop and perform cytogenetic assays to characterize chromosomal breaks, translocations and copy number changes by standard G-band karyotyping, spectral karyotyping (SKY), chromosomal painting and locus-specific FISH. In the second aim, the Core will provide services for automated imaging of DNA damage and response factors, and will apply quantitative PCR-based assays for detection of ssDNA formed by end resection of site-specific DSBs in yeast and mammalian cells, and DNA combing method to measure the length of resection tracks on single molecules from cells treated with irradiation or topoisomerase inhibitors.

总结 基因组不稳定性的分子分析核心将进行最先进的分析， 研究人员在细胞遗传学畸变的分析，和DNA损伤的反应和修复。这些 分析将补充测序结果（例如，检查未被DNA覆盖的重复区域 测序方法），并为复发性基因组不稳定性提供实验验证， 使用基因组方法鉴定的重排（核心C）。此外，核心将执行基于细胞的 检测和定量DNA损伤和DNA修复途径的生物标志物，并测量DNA 修复中间体。拥有一个专门的核心是有益的：1）成本效益和2） 根据每个项目的具体要求定制设计实验。通过集中这些 在两位经验丰富的研究人员Lorraine Symington博士和Shan Zha博士的领导下， 核心将提高生产力的计划项目成员提供必要的服务，所有 项目，并确保数据采集和解释的统一性。核心有两个独立的具体 目标，这是建立在核心领导人的专业知识。目标1的目的是开发和执行 细胞遗传学试验，用于表征染色体断裂、易位和拷贝数变化， 标准G带核型分析、光谱核型分析（SKY）、染色体涂染和基因座特异性FISH。在 第二个目标，核心将提供DNA损伤和响应因子的自动成像服务， 并将应用基于定量PCR的测定来检测由位点特异性末端切除形成的ssDNA， 酵母和哺乳动物细胞中的DSB，以及DNA梳理法测量 来自用辐射或拓扑异构酶抑制剂处理的细胞的单分子。