A novel protective mechanism in hemorrhagic shock

失血性休克的新型保护机制

• 批准号: 10593236
• 负责人: TING C ZHao
• 金额: $ 22.3万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10593236
• 关键词: Anatomy; Animal Model; Animals; Cardiac; Cardiovascular Pathology; Cardiovascular Physiology; Communities; Data; Development; Disease; Echocardiography; Electronics; Employment; Ensure; Experimental Designs; Exposure to; Frequencies; Funding; Grant; Health; Health Care Research; Hemorrhage; Hemorrhagic Shock; Hospitals; Image; In Vitro; Institutes; Knowledge; Magnetic Resonance Imaging; Measurement; Medical; Metabolic Diseases; Methodology; Methods; Monitor; Mus; Myocardial; Noise; Parents; Pathologic; Performance; Physiologic Monitoring; Physiological; Postdoctoral Fellow; Research; Research Personnel; Resolution; Resuscitation; Rhode Island; Rodent; Scientist; Signal Transduction; Solid; System; Techniques; Technology; Testing; Therapeutic; Therapeutic Intervention; Time; Transducers; Trauma; Traumatic injury; Ultrasonography; United States National Institutes of Health; Universities; advanced system; career; cost; data acquisition; design; experimental study; heart function; hemodynamics; high resolution imaging; imaging study; imaging system; improved; in vivo; in vivo monitoring; insight; novel; organ injury; parent grant; pre-clinical; preclinical study; programs; real time monitoring; systemin; tool; ultrasound; undergraduate student

The assessment of myocardial function in vivo following trauma and hemorrhage is implicated as one of the most critical approaches to deciphering physiological mechanism and designing a therapeutic strategy. The precise and accurate determination of the physiological function in preclinical animal models will provide important evidence for developing an effectively therapy and unravelling the deep mechanism(s) attributable for pathological disorders and therapeutic intervention. In vivo non-invasive approaches have served as a powerful technology to elucidate these physiological functions. We and others have extensively used in vitro and invasive methods to determine cardiac functioning in small animal studies, providing crucial analysis of cardiac performance in experimental design. However, those approaches and methodological tools with in vitro and invasive techniques were designed to be performed at the end stage of the study and at the expense of euthanizing animals in the studies, which limits obtaining the in vivo assessment of cardiac performance in a serial manner to monitor the progress of experimental studies. The deficiencies include the limitation of measurements and the lack of real time monitoring. In this parent R01 grant, we will determine cardiac function of mice that are subjected to hemorrhage and resuscitation, in vivo measurement of cardiac function by echocardiographic assessment will be conducted in real time and in a non-invasive manner. We have employed echocardiographic analysis of myocardial performance in mouse using the conventional ultrasound for acquisition of data, however, false negative and positive signals, high background noise of imaging measurement frequently occur due to the limitation of the low frequency of transducer. In order to ensure the rigorousness and solidity of our study, we will request to include the most advanced technology by implementing a high- resolution micro-ultrasound the Vevo® 3100 LT imaging system that is specifically designed for small animals to determine in vivo cardiac function. The advantages with employment of the high resolution ultrasound technology the Vevo® 3100 LT imaging systemin our research not only provide a comprehensive array of accurate and serial analyses of cardiovascular function for the proposed studies, but it will also impact our long- standing focus on studying physiological function in small animals by strengthening our ongoing support, improving the knowledge of trainees, and benefiting the greater research community and NIH-funded research.

创伤和出血后体内心肌功能的评估被认为是 解读生理机制和设计治疗策略的最关键方法。的 在临床前动物模型中精确和准确地测定生理功能将提供 为开发有效的治疗方法和揭示可归因于 病理性疾病和治疗性干预。体内非侵入性方法已经作为一种强有力的 技术来阐明这些生理功能。我们和其他人已经广泛地使用体外和侵入性 在小动物研究中确定心脏功能的方法，提供心脏功能的关键分析。 实验设计中的性能。然而，这些体外和 侵入性技术被设计为在研究的最后阶段进行， 在研究中对动物实施安乐死，这限制了在体内获得心脏性能的评估， 以连续的方式监测实验研究的进展。缺陷包括： 测量和缺乏真实的时间监测。在本研究中，我们将确定心脏功能 在经历出血和复苏的小鼠中，通过在体内测量心脏功能， 超声心动图评估将以真实的时间和非侵入性方式进行。我们已经采用 超声心动图分析小鼠心肌性能的常规超声方法 然而，数据采集存在假阴性和阳性信号、成像测量的高背景噪声 由于传感器低频的限制，经常会发生这种情况。为了确保严格性和 我们的研究，我们将要求包括最先进的技术，通过实施高- Vevo® 3100 LT成像系统专为小型、高分辨率的 动物以确定体内心脏功能。使用高分辨率超声的优势 我们研究中的Vevo® 3100 LT成像系统不仅提供了全面的 准确和系列的心血管功能分析，但它也将影响我们的长期- 通过加强我们的持续支持， 提高受训者的知识，并使更大的研究社区和NIH资助的研究受益。