Metal Ion Transport by the Cation Diffusion Facilitator Family

阳离子扩散促进剂家族的金属离子传输

• 批准号: 10592636
• 负责人: David L. Stokes
• 金额: $ 1.43万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10592636
• 关键词: Bachelor' s Degree; Behavior; Binding; Binding Sites; Biochemistry; Biological; Biological Assay; Biophysics; Cations; Diffusion; Electrophysiology (science); Environment; Event; Exposure to; Family; Funding; Goals; Grant; Group Meetings; Hour; Human Resources; Immunology; Institutes; Ion Channel; Ion Transport; Laboratories; Leadership; Learning; Membrane; Mentors; Metals; Molecular; Molecular Conformation; New York; Preparation; Property; Proteins; Request for Applications; Research; Research Assistant; Research Personnel; Role; Running; Schools; Science; Site; Solid; Spectrum Analysis; Structure; Students; Time; Training Programs; Transport Process; Universities; Work; Zinc; absorption; analytical method; biophysical analysis; biophysical tools; career; career development; design; experimental study; medical schools; member; mutant; parent grant; programs; social; summer program; summer research; symposium; undergraduate research experience; undergraduate student

We are requesting funds to support an undergraduate student researcher during the summer months of 2022. In particular, Charlene Manipon is an undergraduate in the Biochemistry program at New York University and she has recently joined the Stokes lab to pursue a project suitable for an honors thesis associated with her Bachelor's degree. She is currently learning about the background of the project and about the biophysical tools that we use to analyze structure and function of YiiP, which is the main target of the parent grant. Although she is only able to work limited hours during the school year, this supplement will allow her to work full time on this project over the summer. In addition, she will participate in programmatic activities associated with the Summer Program for Undergraduate Researchers, which is run annually by the Vilcek Graduate Institute of Biomolecular Sciences, including career-building seminars, social events and attendance of the Leadership Alliance National Symposium where she will present her work. As a member of the Stokes lab, she will also attend group meetings and Work-in-Progress seminars associated with our graduate program in Biochemistry and Molecular Biophysics as well as with the Ion Channels and Transporters in Immunology. The project will focus on allosteric control of zinc transport by the cation diffusion facilitator YiiP, which is consistent with the aims of the parent grant. In particular, she will use analytical methods such as microscale thermophoresis, atomic absorption spectroscopy, solid supported membrane electrophysiology and fluorimetry to assess zinc binding and transport activity of a variety of mutants. These studies will be designed to understand roles of the three distinct zinc binding sites either in stabilizing the structure of the homodimer or in eliciting allosteric changes associated with transport.

我们正在申请资金来支持暑期本科生研究员 2022 年的几个月。特别是，Charlene Manipon 是生物化学专业的本科生 她在纽约大学攻读博士学位，最近加入斯托克斯实验室开展一个项目 适合与她的学士学位相关的荣誉论文。她目前正在学习 关于该项目的背景以及我们用来分析的生物物理工具 YiiP的结构和功能，这是母基金的主要目标。虽然她只是 能够在学年期间工作有限的时间，该补贴将使她能够全职工作 整个夏天都在这个项目上。此外，她还将参加计划性活动 与本科生研究人员暑期项目相关，该项目每年由 维尔切克生物分子科学研究所，包括职业建设研讨会， 社交活动和出席领导力联盟全国研讨会，她将在那里 展示她的作品。作为斯托克斯实验室的成员，她还将参加小组会议和 与我们的生物化学研究生课程相关的正在进行的研讨会 分子生物物理学以及免疫学中的离子通道和转运蛋白。这 项目将重点关注阳离子扩散促进剂 YiiP 对锌转运的变构控制， 这与家长补助金的目标是一致的。特别是，她将使用分析 微尺度热泳、原子吸收光谱、固体支撑等方法 膜电生理学和荧光测定法评估锌结合和转运活性 各种突变体。这些研究旨在了解三种不同锌的作用 稳定同二聚体结构或引发变构变化的结合位点 与交通有关。

项目成果

Zinc binding alters the conformational dynamics and drives the transport cycle of the cation diffusion facilitator YiiP.

• DOI: 10.1085/jgp.202112873
• 发表时间: 2021-08-02
• 期刊: The Journal of general physiology
• 作者: Lopez-Redondo M;Fan S;Koide A;Koide S;Beckstein O;Stokes DL
• 通讯作者: Stokes DL

Precise force-field-based calculations of octanol-water partition coefficients for the SAMPL7 molecules.

• DOI: 10.1007/s10822-021-00407-4
• 发表时间: 2021-07
• 期刊: Journal of computer-aided molecular design
• 影响因子: 3.5
• 作者: Fan S;Nedev H;Vijayan R;Iorga BI;Beckstein O
• 通讯作者: Beckstein O

Prediction of octanol-water partition coefficients for the SAMPL6-[Formula: see text] molecules using molecular dynamics simulations with OPLS-AA, AMBER and CHARMM force fields.

• DOI: 10.1007/s10822-019-00267-z
• 发表时间: 2020-05
• 期刊: Journal of computer-aided molecular design
• 影响因子: 3.5
• 作者: Fan S;Iorga BI;Beckstein O
• 通讯作者: Beckstein O