Metal Ion Transport by the Cation Diffusion Facilitator Family

阳离子扩散促进剂家族的金属离子传输

• 批准号: 10592636
• 负责人: David L. Stokes
• 金额: $ 1.43万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10592636
• 关键词: Bachelor' s Degree; Behavior; Binding; Binding Sites; Biochemistry; Biological; Biological Assay; Biophysics; Cations; Diffusion; Electrophysiology (science); Environment; Event; Exposure to; Family; Funding; Goals; Grant; Group Meetings; Hour; Human Resources; Immunology; Institutes; Ion Channel; Ion Transport; Laboratories; Leadership; Learning; Membrane; Mentors; Metals; Molecular; Molecular Conformation; New York; Preparation; Property; Proteins; Request for Applications; Research; Research Assistant; Research Personnel; Role; Running; Schools; Science; Site; Solid; Spectrum Analysis; Structure; Students; Time; Training Programs; Transport Process; Universities; Work; Zinc; absorption; analytical method; biophysical analysis; biophysical tools; career; career development; design; experimental study; medical schools; member; mutant; parent grant; programs; social; summer program; summer research; symposium; undergraduate research experience; undergraduate student

We are requesting funds to support an undergraduate student researcher during the summer months of 2022. In particular, Charlene Manipon is an undergraduate in the Biochemistry program at New York University and she has recently joined the Stokes lab to pursue a project suitable for an honors thesis associated with her Bachelor's degree. She is currently learning about the background of the project and about the biophysical tools that we use to analyze structure and function of YiiP, which is the main target of the parent grant. Although she is only able to work limited hours during the school year, this supplement will allow her to work full time on this project over the summer. In addition, she will participate in programmatic activities associated with the Summer Program for Undergraduate Researchers, which is run annually by the Vilcek Graduate Institute of Biomolecular Sciences, including career-building seminars, social events and attendance of the Leadership Alliance National Symposium where she will present her work. As a member of the Stokes lab, she will also attend group meetings and Work-in-Progress seminars associated with our graduate program in Biochemistry and Molecular Biophysics as well as with the Ion Channels and Transporters in Immunology. The project will focus on allosteric control of zinc transport by the cation diffusion facilitator YiiP, which is consistent with the aims of the parent grant. In particular, she will use analytical methods such as microscale thermophoresis, atomic absorption spectroscopy, solid supported membrane electrophysiology and fluorimetry to assess zinc binding and transport activity of a variety of mutants. These studies will be designed to understand roles of the three distinct zinc binding sites either in stabilizing the structure of the homodimer or in eliciting allosteric changes associated with transport.

我们要求资金在夏季支持本科生研究人员 尤其是2022年的月份，曼彭夏琳是生物化学的本科生 纽约大学的课程，她最近加入了Stokes Lab进行项目 适合与她的学士学位相关的荣誉论文。她目前正在学习 关于项目的背景以及我们用于分析的生物物理工具 Yiip的结构和功能，这是父授予的主要目标。虽然她只是 能够在学年中工作有限的时间，这种补充剂将使她能够全职工作 在夏季这个项目上。此外，她将参加程序化活动 与本科研究人员的夏季计划有关，该计划每年由 维尔塞克生物分子科学研究所，包括职业生涯研讨会， 社交活动和领导联盟国家研讨会的出席 介绍她的作品。作为Stokes Lab的成员，她还将参加小组会议， 与我们的生物化学研究生课程相关的进行工作研讨会 分子生物物理学以及免疫学中的离子通道和转运蛋白。这 项目将重点介绍阳离子扩散促进器Yiip对锌传输的变构控制， 这与父母赠款的目标一致。特别是，她将使用分析 诸如微观嗜热，原子吸收光谱，固体支持的方法 膜电生理学和荧光法，以评估A的锌结合和转运活性 多种突变体。这些研究将旨在了解三个不同锌的角色 结合位点可以稳定同型二聚体的结构或引起变形的变化 与运输相关。

项目成果

Zinc binding alters the conformational dynamics and drives the transport cycle of the cation diffusion facilitator YiiP.

• DOI: 10.1085/jgp.202112873
• 发表时间: 2021-08-02
• 期刊: The Journal of general physiology
• 作者: Lopez-Redondo M;Fan S;Koide A;Koide S;Beckstein O;Stokes DL
• 通讯作者: Stokes DL

Precise force-field-based calculations of octanol-water partition coefficients for the SAMPL7 molecules.

• DOI: 10.1007/s10822-021-00407-4
• 发表时间: 2021-07
• 期刊: Journal of computer-aided molecular design
• 影响因子: 3.5
• 作者: Fan S;Nedev H;Vijayan R;Iorga BI;Beckstein O
• 通讯作者: Beckstein O

Prediction of octanol-water partition coefficients for the SAMPL6-[Formula: see text] molecules using molecular dynamics simulations with OPLS-AA, AMBER and CHARMM force fields.

• DOI: 10.1007/s10822-019-00267-z
• 发表时间: 2020-05
• 期刊: Journal of computer-aided molecular design
• 影响因子: 3.5
• 作者: Fan S;Iorga BI;Beckstein O
• 通讯作者: Beckstein O