Mouse Breeding and Procedure Core

小鼠育种和程序核心

• 批准号: 10616534
• 负责人: ANN MARIE SCHMIDT
• 金额: $ 38.55万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-05-01 至 2027-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10616534
• 关键词: Adipose tissue; Age; Aorta; Atherosclerosis; Bone Marrow Transplantation; Breeding; Cardiometabolic Disease; Dual-Energy X-Ray Absorptiometry; Functional disorder; Gender; Genotype; Hepatic Stellate Cell; Hepatocyte; Individual; Kupffer Cells; Liver; Macrophage; Magnetic Resonance Imaging; Mus; Parabiosis; Partner in relationship; Procedures; Quality Control; Randomized; Reproducibility; Scanning; Services; Tail; Testing; Tissue Transplantation; Transplantation Surgery; Weaning; extracellular vesicles; glucose tolerance; imaging study; insulin tolerance; irradiation; member; programs; sex; ultrasound

Summary The Mouse Breeding and Procedure Core (Core C) is essential for the smooth functioning of the Program Project. Core C has two major functions. First, Core C will provide and oversee mouse breeding and genotyping services. Mice will be bred centrally for all three projects of the Program Project in order to maximize quality control and consistency of mouse lines, and to maximize efficiency, particularly for lines shared across the three Projects. Mice will be mated, born, separated, and weaned by the Core members. The Core will direct tails for genotyping to NYU Core for Genotyping Services. Once genotyped, Core C will allocate mice to either further breeding or to experimentation depending on the needs and the genotype/sexes of mice obtained. Core C will distribute all mice for studies to the three Projects by age 6 weeks. As indicated in the individual Projects, Core C, through Core A, implements randomization and strategies that will be essential for rigor and reproducibility and protection against bias. Second, Core C will coordinate and perform mouse procedures. Shared procedures across the Program Project to be performed and overseen by Core C include: aorta transplantation surgery, lethal irradiation and bone marrow transplantation; adipose tissue transplantation; parabiosis; glucose and insulin tolerance tests; imaging studies (liver ultrasound and magnetic resonance imaging and DEXA scanning); isolation of primary cells from the liver, including hepatocytes, Kupffer cells and hepatic stellate cells and isolation of extracellular vesicles. Core C will serve all three projects of the Program Project over all five years.

总结 小鼠育种和程序核心（核心C）对于该计划的顺利运作至关重要 项目Core C有两个主要功能。首先，核心C将提供和监督小鼠育种和基因分型 服务为了最大限度地提高质量，计划项目的所有三个项目都将集中饲养小鼠 控制和一致性的鼠标线，并最大限度地提高效率，特别是对线共享的三个 项目小鼠将由核心成员交配、出生、分离和断奶。核心将引导尾巴， 基因分型到NYU Core for Genotyping Services一旦基因分型，核心C将小鼠分配到进一步的 根据需要和获得的小鼠的基因型/性别进行育种或实验。核心C将 按6周龄将所有小鼠分配给三个项目进行研究。如个别项目所示， 通过核心A，C实现了随机化和策略，这对严谨性和可重复性至关重要 和防止偏见。其次，Core C将协调和执行鼠标程序。共享程序 由核心C执行和监督的整个计划项目包括：主动脉移植手术， 致死性照射骨髓移植;脂肪组织移植;联体共生;葡萄糖和胰岛素 耐受性试验;成像研究（肝脏超声和磁共振成像以及DEXA扫描）; 从肝脏分离原代细胞，包括肝细胞、枯否细胞和肝星状细胞，并分离 细胞外囊泡的结构。核心C将在所有五年内为该计划项目的所有三个项目提供服务。