Perivascular Fibroblast-Endothelium Interactions in Hypertension and Cerebral Ischemia
高血压和脑缺血中血管周围成纤维细胞-内皮细胞的相互作用
基本信息
- 批准号:10598600
- 负责人:
- 金额:$ 20.19万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-04-01 至 2024-03-31
- 项目状态:已结题
- 来源:
- 关键词:AcuteAffinity ChromatographyAgeAlzheimer&aposs DiseaseAngiogenesis InhibitorsBioinformaticsBlood VesselsBrainCell CommunicationCerebral IschemiaCerebral cortexCerebrumChronicCognition DisordersCollagenCommon carotid arteryContralateralDataEndothelial CellsEndotheliumExtracellular MatrixFibroblastsGene ExpressionGenesGenomicsGoalsHypertensionImpaired cognitionIn Situ HybridizationInflammationInflammatoryInterventionIpsilateralIschemiaMatrix MetalloproteinasesMessenger RNAMethodsMicrovascular DysfunctionMusOutcomeOxidative StressPathogenesisPathologicPeptide HydrolasesProcessReninResistanceRibosomesSideSmooth Muscle MyocytesStimulusTestingTimeTranscriptTransgenic OrganismsTranslatingVascular Cognitive ImpairmentVascular Endothelial CellVascular EndotheliumWild Type Mouseangiogenesisarterioleartery occlusionblood-brain barrier permeabilizationcell typecerebrovascularcognitive functioncytokinedensitydifferential expressionendothelial dysfunctionimmunoregulationinsightmouse modelnew therapeutic targetnovelpublic health relevanceresponsesingle-cell RNA sequencingtranscriptometranscriptome sequencingvascular injury
项目摘要
PROJECT SUMMARY (Description)
Perivascular fibroblasts (PVFs) and vascular endothelium are important responders to hypertension and
cerebral ischemia. Long-term aberrant interactions of PVFs and endothelium may also promote vascular
cognitive disorders (VCD), but the mechanisms remain poorly understood. This project will use PVF-specific
single-cell RNA-Seq and vascular endothelium-specific or TRAP-Seq to test the impacts of hypertension and
cerebral ischemia on gene expression. Outcomes of this project may suggest novel therapeutic targets.
Aim 1 will test the transcriptome changes of endothelial cells in a mouse model of chronic hypertension
(ReninTg) with and without partial cerebral ischemia.
Aim 2 will use bitransgenic ReninTg and Pdgfra-H2BGFP mice, with and without partial cerebral ischemia, to
isolate PVFs for single-cell RNA-Seq comparison.
In summary, this project will use open-ended genomic methods to correlate, for the first time, the transcriptome
adaptations by PVFs and vascular endothelium to hypertension and combined hypertension/ischemia.
项目概要(说明)
血管周成纤维细胞(PVF)和血管内皮细胞是高血压和高血压病的重要反应者。
脑缺血PVF和内皮细胞的长期异常相互作用也可能促进血管生成。
认知障碍(VCD),但机制仍然知之甚少。本项目将使用PVF专用
单细胞RNA-Seq和血管内皮特异性或TRAP-Seq,以测试高血压的影响,
脑缺血对基因表达的影响该项目的结果可能提出新的治疗靶点。
目的1检测慢性高血压小鼠模型内皮细胞转录组的变化
(ReninTg)伴和不伴部分脑缺血。
目的2将使用双转基因ReninTg和Pdgfra-H2 BGFP小鼠,有和没有部分脑缺血,
分离PVF用于单细胞RNA-Seq比较。
总之,该项目将首次使用开放式基因组方法关联转录组,
PVF和血管内皮对高血压和高血压/缺血联合的适应。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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{{ truncateString('Chia-Yi Kuan', 18)}}的其他基金
Monocyte-Derived Microglia in Development and after Neonatal Brain Injury
发育中和新生儿脑损伤后的单核细胞衍生的小胶质细胞
- 批准号:
10593385 - 财政年份:2023
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Treating neurotoxicity and cognitive deficits due to hyperphosphorylated tau.
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10815399 - 财政年份:2023
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$ 20.19万 - 项目类别:
Perivascular Fibroblast-Endothelium Interactions in Hypertension and Cerebral Ischemia
高血压和脑缺血中血管周围成纤维细胞-内皮细胞的相互作用
- 批准号:
10463370 - 财政年份:2022
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$ 20.19万 - 项目类别:
Neutrophils and Monocytes in Pediatric Ischemic Stroke
小儿缺血性中风中的中性粒细胞和单核细胞
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10629365 - 财政年份:2022
- 资助金额:
$ 20.19万 - 项目类别:
Neutrophils and Monocytes in Pediatric Ischemic Stroke
小儿缺血性中风中的中性粒细胞和单核细胞
- 批准号:
10529933 - 财政年份:2022
- 资助金额:
$ 20.19万 - 项目类别:
Creatine Transporter Deficiency and Brain Energetics
肌酸转运蛋白缺乏和脑能量学
- 批准号:
10442483 - 财政年份:2018
- 资助金额:
$ 20.19万 - 项目类别:
Creatine Transporter Deficiency and Brain Energetics
肌酸转运蛋白缺乏和脑能量学
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10217271 - 财政年份:2018
- 资助金额:
$ 20.19万 - 项目类别:
Microglia- Monocyte Interactions following Perinatal Brain Injury
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- 批准号:
9198866 - 财政年份:2016
- 资助金额:
$ 20.19万 - 项目类别:
Microglia- Monocyte Interactions following Perinatal Brain Injury
围产期脑损伤后小胶质细胞-单核细胞相互作用
- 批准号:
9110552 - 财政年份:2016
- 资助金额:
$ 20.19万 - 项目类别:
HIF1a in Neonatal Hypoxic-Ischemic Brain Injury and White-Matter Vulnerability
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- 批准号:
8905538 - 财政年份:2015
- 资助金额:
$ 20.19万 - 项目类别:
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