Developing Activatable Fluorescent Flavonoids for Vascular Imaging

开发用于血管成像的可激活荧光黄酮类化合物

• 批准号: 10579722
• 负责人: Yi Pang
• 金额: $ 43.94万
• 依托单位: UNIVERSITY OF AKRON
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2026-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10579722
• 关键词: Acute; Affect; Angiography; Animal Model; Binding; Biological; Blood - brain barrier anatomy; Blood Stains; Blood Vessels; Blood flow; Brain Ischemia; Cardiovascular Diseases; Cells; Chronic; Complex; Development; Diagnosis; Diameter; Dimensions; Disease; Drug usage; Dyes; Excision; Exhibits; Extravasation; FDA approved; Fishes; Flavonoids; Fluorescein Angiography; Fluorescence; Fluorescent Dyes; Fluorescent Probes; Goals; Hematological Disease; Hydrophobicity; Image; Inflammation; Injections; Kidney Diseases; Length; Libraries; Life; Malignant Neoplasms; Modeling; Molecular; Molecular Weight; Organism; Penetration; Prevention; Process; Property; Reagent; Research; Series; Signal Transduction; Sodium Fluorescein; Stains; Structure; Tissues; Ultraviolet Rays; Vascular Diseases; Vascular System; Vascularization; Zebrafish; absorption; angiogenesis; aqueous; body system; contrast imaging; design; electron donor; experimental study; flavonoid probes; fluorescence-guided surgery; fruits and vegetables; human disease; improved; in vivo imaging; model organism; novel; ophthalmic examination; preservation; response; small molecule; success; user-friendly

Developing Activatable Fluorescent Flavonoids for Vascular Imaging Project Summary/Abstract: During the imaging applications, fluorescent probes may be away from their binding targets, or in excess, thus giving background signals that affect the quality of imaging. Removal of the background signals often requires the post-staining washing to remove the excess dye, which could be a limiting factor for some applications. The proposed research seeks to improve the imaging process by developing flavonoid-based imaging reagents that give nearly no fluorescence in aqueous solution. However, the fluorescence of these dyes will be greatly enhanced during target-binding interaction, thus eliminating the need for post-staining washing step. When being applied to wild-type zebrafish, the developed dye can penetrate into the fish’s body and selectively target blood vessels, without using labor-intensive “dye injection”. Because zebrafish has become a favorite model organism for studying vertebrate development and various human diseases, discovery of these new imaging reagents for in vivo imaging could have large impact in the field of biological studies. The experimental study aims to discover and synthesize these probes that exhibit selective binding toward blood vessels. Discovery of such imaging reagents could lead to user-friendly probes for in vivo imaging of blood vessels, which could facilitate the related study by using zebrafish model.

血管显影用可激活荧光类黄酮的研究进展 项目摘要/摘要： 在成像应用期间，荧光探针可能远离其结合目标，或过度， 从而给出了影响成像质量的背景信号。背景信号的去除通常 需要染色后的洗涤来去除多余的染料，这可能是一些染料的限制因素 申请。这项拟议的研究试图通过开发黄酮类化合物来改善成像过程。 在水溶液中几乎没有荧光的成像试剂。然而，这些物质的荧光 在靶标结合过程中，染料的作用将大大增强，从而消除了染色后的需要 洗涤步骤。当应用于野生斑马鱼时，开发的染料可以渗透到鱼的体内 并选择性地靶向血管，而不需要使用劳动密集型的“染料注射”。因为斑马鱼有 成为研究脊椎动物发育和各种人类疾病的最受欢迎的模式生物， 这些用于活体成像的新显像剂的发现可能会对生物领域产生重大影响 学习。这项实验研究旨在发现和合成这些具有选择性结合的探针 朝向血管。这类显像剂的发现可能导致使用方便的活体探针 血管成像，为斑马鱼模型的相关研究提供方便。