Developing Activatable Fluorescent Flavonoids for Vascular Imaging

血管显像用可激活荧光素的研制 项目概要/摘要： 在成像应用期间，荧光探针可以远离它们的结合靶，或者过量， 从而给出影响成像质量的背景信号。背景信号的去除通常 需要染色后清洗以去除过量的染料，这可能是一些人的限制因素。 应用.拟议的研究旨在通过开发基于黄酮类化合物的 在水溶液中几乎不产生荧光的成像试剂。然而，这些荧光 染料在靶结合相互作用期间将被大大增强，从而消除了后染色的需要。 洗涤步骤。当应用于野生型斑马鱼时，开发的染料可以渗透到鱼的体内 并且选择性地靶向血管，而不使用劳动密集型的“染料注射”。因为斑马鱼 成为研究脊椎动物发育和各种人类疾病的最受欢迎的模式生物， 这些用于体内成像的新成像试剂的发现可能在生物医学领域具有很大的影响。 问题研究实验研究的目的是发现和合成这些具有选择性结合的探针 朝向血管。这种成像试剂的发现可能会导致用户友好的探针，用于体内 血管成像，这将有助于利用斑马鱼模型进行相关研究。

Developing Activatable Fluorescent Flavonoids for Vascular Imaging Project Summary/Abstract: During the imaging applications, fluorescent probes may be away from their binding targets, or in excess, thus giving background signals that affect the quality of imaging. Removal of the background signals often requires the post-staining washing to remove the excess dye, which could be a limiting factor for some applications. The proposed research seeks to improve the imaging process by developing flavonoid-based imaging reagents that give nearly no fluorescence in aqueous solution. However, the fluorescence of these dyes will be greatly enhanced during target-binding interaction, thus eliminating the need for post-staining washing step. When being applied to wild-type zebrafish, the developed dye can penetrate into the fish’s body and selectively target blood vessels, without using labor-intensive “dye injection”. Because zebrafish has become a favorite model organism for studying vertebrate development and various human diseases, discovery of these new imaging reagents for in vivo imaging could have large impact in the field of biological studies. The experimental study aims to discover and synthesize these probes that exhibit selective binding toward blood vessels. Discovery of such imaging reagents could lead to user-friendly probes for in vivo imaging of blood vessels, which could facilitate the related study by using zebrafish model.