The biofilm matrix of P. aeruginosa

铜绿假单胞菌的生物膜基质

• 批准号: 10579223
• 负责人: Michael MacCoss
• 金额: $ 53.99万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-02-22 至 2025-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10579223
• 关键词: Acute; Amino Acid Sequence; Bacterial Infections; Binding; Biology; Biotinylation; CT2584 HMS; Carbohydrates; Caseins; Cells; Chemicals; Chemistry; Chronic; Clinical; Communities; Cystic Fibrosis sputum; Data; Disease; Disease Outcome; Elastases; Event; Extracellular Matrix; Family suidae; Host Defense; Human; Immunohistochemistry; In Vitro; Laboratories; Lead; Link; Lung; Microbial Biofilms; Modeling; Mus; Mutation; Nucleic Acids; Nutritional; Pathogenesis; Pathogenicity; Peptide Hydrolases; Play; Polymers; Polysaccharides; Process; Production; Property; Protease Inhibitor; Proteins; Proteolytic Processing; Pseudomonas aeruginosa; Regulation; Research; Role; Sampling; Structure; Susceptibility Gene; Testing; Variant; Wound Infection; antimicrobial; chronic infection; extracellular; fitness; in vivo; neutrophil; novel; overexpression; protease IV

Project Summary Biofilm formation has been linked to many chronic bacterial infections. Thus, significant research has been directed towards understanding the basic biology behind biofilm formation. Biofilms produce an extracellular matrix that functions, in part, to hold the community together. Pseudomonas aeruginosa represents a paradigm species for the study of biofilms in the laboratory. The regulation of matrix production and the carbohydrate component of the matrix have both been examined. However, the protein component of the biofilm matrix has been relatively understudied. Our groups identified a biofilm matrix protein for P. aeruginosa, CdrA. CdrA provides structural integrity through extracellular interactions with the matrix polysaccharide Psl. Outside of matrix proteins that provide structural functions, we predict that matrix associated proteins can play both nutritional and protective roles for the community. This project will initially focus on CdrA and its role in the matrix. We will then characterize ecotin a matrix bound protease inhibitor and three matrix-associated proteases. Finally, we will identify new matrix proteins that associate with eps component of the biofilm matrix.

项目总结