The biofilm matrix of P. aeruginosa
铜绿假单胞菌的生物膜基质
基本信息
- 批准号:10579223
- 负责人:
- 金额:$ 53.99万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2019
- 资助国家:美国
- 起止时间:2019-02-22 至 2025-01-31
- 项目状态:未结题
- 来源:
- 关键词:AcuteAmino Acid SequenceBacterial InfectionsBindingBiologyBiotinylationCT2584 HMSCarbohydratesCaseinsCellsChemicalsChemistryChronicClinicalCommunitiesCystic Fibrosis sputumDataDiseaseDisease OutcomeElastasesEventExtracellular MatrixFamily suidaeHost DefenseHumanImmunohistochemistryIn VitroLaboratoriesLeadLinkLungMicrobial BiofilmsModelingMusMutationNucleic AcidsNutritionalPathogenesisPathogenicityPeptide HydrolasesPlayPolymersPolysaccharidesProcessProductionPropertyProtease InhibitorProteinsProteolytic ProcessingPseudomonas aeruginosaRegulationResearchRoleSamplingStructureSusceptibility GeneTestingVariantWound Infectionantimicrobialchronic infectionextracellularfitnessin vivoneutrophilnoveloverexpressionprotease IV
项目摘要
Project Summary
Biofilm formation has been linked to many chronic bacterial infections. Thus, significant research has been
directed towards understanding the basic biology behind biofilm formation. Biofilms produce an extracellular
matrix that functions, in part, to hold the community together. Pseudomonas aeruginosa represents a
paradigm species for the study of biofilms in the laboratory. The regulation of matrix production and the
carbohydrate component of the matrix have both been examined. However, the protein component of the
biofilm matrix has been relatively understudied. Our groups identified a biofilm matrix protein for P.
aeruginosa, CdrA. CdrA provides structural integrity through extracellular interactions with the matrix
polysaccharide Psl. Outside of matrix proteins that provide structural functions, we predict that matrix
associated proteins can play both nutritional and protective roles for the community. This project will initially
focus on CdrA and its role in the matrix. We will then characterize ecotin a matrix bound protease inhibitor and
three matrix-associated proteases. Finally, we will identify new matrix proteins that associate with eps
component of the biofilm matrix.
项目概要
生物膜的形成与许多慢性细菌感染有关。因此,重要的研究已经
旨在了解生物膜形成背后的基本生物学。生物膜产生细胞外
矩阵在一定程度上起到了将社区凝聚在一起的作用。铜绿假单胞菌代表
实验室生物膜研究的范例物种。基质产生的调节和
基质的碳水化合物成分均已被检查。然而,蛋白质成分
生物膜基质的研究相对较少。我们的小组确定了 P. 的生物膜基质蛋白。
铜绿假单胞菌,CdrA。 CdrA 通过细胞外与基质的相互作用提供结构完整性
多糖Psl。除了提供结构功能的基质蛋白之外,我们预测基质
相关蛋白质可以为社区发挥营养和保护作用。该项目最初将
重点关注 CdrA 及其在基质中的作用。然后我们将表征大肠杆菌素(基质结合蛋白酶抑制剂)和
三种基质相关蛋白酶。最后,我们将鉴定与 eps 相关的新基质蛋白
生物膜基质的组成部分。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael MacCoss其他文献
Michael MacCoss的其他文献
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{{ truncateString('Michael MacCoss', 18)}}的其他基金
Seattle Quant: A Resource for the Skyline Software Ecosystem
Seattle Quant:Skyline 软件生态系统的资源
- 批准号:
10609502 - 财政年份:2021
- 资助金额:
$ 53.99万 - 项目类别:
Seattle Quant: A Resource for the Skyline Software Ecosystem
Seattle Quant:Skyline 软件生态系统的资源
- 批准号:
10400105 - 财政年份:2021
- 资助金额:
$ 53.99万 - 项目类别:
Seattle Quant: A Resource for the Skyline Software Ecosystem
Seattle Quant:Skyline 软件生态系统的资源
- 批准号:
10189938 - 财政年份:2021
- 资助金额:
$ 53.99万 - 项目类别:
Project 1: Discovery of proteins with altered abundance and stability
项目 1:发现丰度和稳定性发生改变的蛋白质
- 批准号:
10359192 - 财政年份:2020
- 资助金额:
$ 53.99万 - 项目类别:
Project 1: Discovery of proteins with altered abundance and stability
项目 1:发现丰度和稳定性发生改变的蛋白质
- 批准号:
10573256 - 财政年份:2020
- 资助金额:
$ 53.99万 - 项目类别:
Next Generation Translational Proteomics for Alzheimer's and Related Dementias
阿尔茨海默氏症和相关痴呆症的下一代转化蛋白质组学
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10573244 - 财政年份:2020
- 资助金额:
$ 53.99万 - 项目类别:
Next Generation Translational Proteomics for Alzheimer's and Related Dementias
阿尔茨海默氏症和相关痴呆症的下一代转化蛋白质组学
- 批准号:
10359187 - 财政年份:2020
- 资助金额:
$ 53.99万 - 项目类别:
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