Laboratory Core

实验室核心

• 批准号: 10242676
• 负责人: John T West
• 金额: $ 0.26万
• 依托单位: UNIVERSITY OF NEBRASKA LINCOLN
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2021-10-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10242676
• 关键词: AIDS related cancer; AIDS with Kaposi' s sarcoma; Acquired Immunodeficiency Syndrome; Africa; Africa South of the Sahara; African; Antigens; Archives; Area; Biological; Biometry; Biopsy; Blood Cells; Breast; Cancer Etiology; Clinical; Coupled; Data; Databases; Diagnosis; Disease; Early identification; Economic Burden; Epidemic; Epidemiology; Equipment and supply inventories; Etiology; Eye; Frequencies; Funding; Genetic; Genotype; Goals; HIV; HIV Infections; HIV-1; Head and neck structure; Herpesviridae Infections; High Prevalence; Histopathology; Human Herpesvirus 8; Human Papillomavirus; Human papilloma virus infection; Immunodiagnostics; Immunohistochemistry; Incidence; Individual; Infection; Information Storage; Infrastructure; Institutes; Kaposi Sarcoma; Laboratories; Life Style; Malignant Neoplasms; Malignant neoplasm of lung; Malignant neoplasm of prostate; Molecular; Molecular Biology; Nebraska; Neoplasms; Pathogenesis; Pathology; Patients; Pattern; Pilot Projects; Plasma; Population; Predisposition; Prevalence; Procedures; Process; Productivity; Prostate; Quality of life; Records; Research; Research Personnel; Research Project Grants; Research Training; Risk; Sampling; Scientist; Smoking; Squamous Cell; Structure; Teaching Hospitals; Tissues; Training; UV Radiation Exposure; United States; Universities; Viral; Virus Replication; Woman; Zambia; analysis pipeline; anticancer research; antiretroviral therapy; base; biological specimen archives; cancer risk; career; co-infection; computerized; demographics; design; experience; health management; improved; interest; leukemia; melanoma; men; molecular diagnostics; multidisciplinary; ocular surface squamous neoplasia; operation; peripheral blood; programs; research study; sex; skills; socioeconomics; training opportunity; tumor; tumorigenesis; uptake; viral detection; virtual

PROJECT SUMMARY/ABSTRACT Sub-Saharan Africans living with HIV infection experience enhanced susceptibility to malignancies even when the individuals are on anti-retroviral therapy (ART) and have fully suppressed HIV-1 viral replication in the peripheral blood. Understanding and reducing the bases of this enhanced cancer risk is a critical next step to improve the quality of life and reduce the economic burden of health management in this region devastated by the AIDS epidemic. In Africa, cancers largely result from infectious etiologies. Squamous cell neoplasias of the head and neck, and particularly those of the eye, are associated with HIV-1 infection in Africa and may result from co-infection with human papilloma viruses (HPV). Likewise, Kaposi’s sarcoma (KS), the most common HIV-associated malignancy in Zambian men and the third leading HIV-associated malignancy in Zambian women, is caused by Kaposi’s Sarcoma Herpesvirus (KSHV)/Human Herpesvirus-8 (HHV-8). Thus, high incidence cancers caused by KSHV, and potentially by HPV, are occurring at higher frequencies in HIV-1 co- infected individuals who appear to be controlling HIV-1 disease through ART. In order to more effectively treat these malignancies, researchers need to understand why this is occurring. The goal of the Zambian AIDS Malignancies Diagnosis and Pathogenesis Program (ZAMDAPP)’s Lab Core is to provide integrated molecular, immunological, and diagnostic support in parallel with ZAMDAPP’s Biostatistics and Epidemiology Core for the consortium-funded research projects and training initiatives, and to further develop capacity and infrastructure to conduct cancer research in Zambia. To accomplish this goal, the core will 1) establish and transfer molecular procedures in support of early KS diagnosis and HPV detection; 2) develop an effective specimen archive, coupled with computerized information storage, to facilitate sharing exchange and analyses pipelines for study data; and 3) provide or facilitate training opportunities to increase essential research skills and capacities for Zambians with interests in HIV-associated malignancy research. The core will be staffed by experienced multidisciplinary basic scientists and clinical lab scientists and will have two hubs of operation: one at the University Teaching Hospital in Lusaka, Zambia and one in the United States at the University of Nebraska-Lincoln.

项目总结/摘要 感染艾滋病毒的撒哈拉以南非洲人对恶性肿瘤的易感性增加， 这些人正在接受抗逆转录病毒治疗（ART），并已完全抑制了HIV-1病毒在体内的复制。 外周血了解和减少这种增加的癌症风险的基础是关键的下一步， 改善生活质量，减轻这一地区卫生管理的经济负担， 艾滋病的流行。在非洲，癌症主要是由传染性病因引起的。鳞状细胞瘤 头部和颈部，特别是眼睛，与非洲的HIV-1感染有关， 与人乳头瘤病毒（HPV）共同感染。同样，卡波西肉瘤（KS），最常见的 赞比亚男性HIV相关恶性肿瘤和赞比亚第三大HIV相关恶性肿瘤 女性，由卡波西肉瘤疱疹病毒（KSHV）/人类疱疹病毒-8（HHV-8）引起。因此，高 由KSHV和可能由HPV引起的癌症的发病率在HIV-1共感染者中以更高的频率发生， 感染者似乎正在通过抗逆转录病毒疗法控制HIV-1疾病。为了更有效地治疗 对于这些恶性肿瘤，研究人员需要了解为什么会发生这种情况。赞比亚艾滋病的目标 肿瘤诊断和发病机制计划（ZAMDAPP）的实验室核心是提供整合的分子， 与ZAMDAPP的生物统计学和流行病学核心并行， 联合会资助的研究项目和培训举措，并进一步发展能力和基础设施 在赞比亚进行癌症研究。为了实现这一目标，核心将1）建立和转移 支持早期KS诊断和HPV检测的分子程序; 2）开发有效的标本 档案，加上计算机化的信息储存，以促进交流和分析渠道 提供或促进培训机会，以提高基本的研究技能， 为对艾滋病毒相关恶性肿瘤研究感兴趣的赞比亚人提供能力。核心人员将由 经验丰富的多学科基础科学家和临床实验室科学家，并将有两个中心的运作： 一个在赞比亚卢萨卡的大学教学医院，一个在美国的 内布拉斯加-林肯