Research Supplements to Promote Diversity in Vascular Graft Research

促进血管移植研究多样性的研究补充

• 批准号: 10619171
• 负责人: Edith Tzeng
• 金额: $ 8.71万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10619171
• 关键词: African American; Animal Model; Applications Grants; Arteries; Autologous; Autologous Transplantation; Blood Vessels; Bypass; Cardiovascular Diseases; Carotid Arteries; Cells; Elements; Fiber; Future; Goals; Gold; Grant; In Situ; Intervention; Minority Participation; Modeling; Morbidity - disease rate; Research; Sheep; Site; Testing; Vascular Graft; clinical translation; design; doctoral student; implantation; minority student; parent grant; pre-clinical; senescence; single-cell RNA sequencing; transcriptome sequencing

Abstract The parent grant of this application is R01HL159427 entitled “Elastic, Degradable Vascular Grafts with Helical Microfibers”. The goal of the parent grant is to design a cell-free degradable graft that the host can transform into an autologous vascular conduit. A major aim of the grant is to investigate host remodeling of long microfibrous grafts in a sheep carotid artery model. We performed five implantations in senescent sheep. We learned that the graft needed to be more robust for clinical translation. The research focus of this supplement application is to eliminate fiber stacking and increase fiber fusion at the junction as means to make the vascular graft more robust. Moreover, the Co-I of the parent grant, Dr. Iwijn De Vlaminck, informed the team of a recent advancement on single cell RNA sequencing in sheep. This will greatly enrich the information we gather from our sheep study. Therefore, the research aspect of this supplement will focus on the following specific aims in the next 3 years: 1. Eliminate fiber stacking and induce fiber fusion at the junctions in the graft. 2. Identify the type and potential origin of the cells populating the grafts in the sheep study using single cell RNA sequencing. The design of electrowritten vascular grafts and the study of host remodeling are key aspects of the parent grant. However, neither the study of fiber stacking and fusion, nor RNA sequencing of the sheep graft is proposed in the parent grant. We recognize these as important elements of the study after the project initiation. The research proposed in this supplemental application was not deleted by the initial review group. Halle Welch, a first year African American Ph.D. student, is very passionate about the vascular graft project. This supplement will increase the participation of minority students in vascular graft research and prepare Halle well for a future in academic research. Thus, this supplement request is consistent with the spirit of a diversity supplement.

摘要 本申请的母授权是题为“具有血管的弹性可降解血管移植物”的R 01 HL 159427。 螺旋微纤维”。父母资助的目标是设计一种无细胞的可降解移植物， 变成一个自体血管管道该补助金的一个主要目的是研究宿主重塑， 在绵羊颈动脉模型中的长微纤维移植物。我们在衰老的绵羊中进行了五次移植。 我们了解到，移植物需要更坚固才能用于临床移植。本研究的重点是 补充应用是消除纤维堆积并增加连接处的纤维融合， 血管移植物更坚固。此外，母公司赠款的Co-I，Iwijn De Vlaminck博士，告知 一个研究小组最近在绵羊单细胞RNA测序方面取得了进展。这将极大地丰富信息 从我们的绵羊研究中得出的结论因此，本补编的研究方面将集中在以下几个方面 未来三年的具体目标： 1.消除纤维堆积并诱导移植物连接处的纤维融合。 2.在绵羊研究中，使用单克隆抗体鉴定移植物中细胞的类型和潜在来源。 细胞RNA测序。 电写入血管移植物的设计和宿主重塑的研究是母体的关键方面 格兰特.然而，无论是纤维堆积和融合的研究，还是绵羊移植物的RNA测序， 在父母补助金中提出。我们认为这些是项目启动后研究的重要内容。 初始审查小组未删除本补充申请中提出的研究。Halle 韦尔奇，第一年的非洲裔美国人博士。学生，对血管移植项目充满热情。这 补充将增加少数民族学生参与血管移植研究，并为哈雷做好准备 为了学术研究的未来因此，这一补充请求符合多样性的精神 补充.