A Genetic Engineering Toolbox for Marmosets (GETMarm): Development and optimization of genome editing and assisted reproduction techniques for marmoset models

狨猴基因工程工具箱 (GETMarm):狨猴模型基因组编辑和辅助生殖技术的开发和优化

基本信息

  • 批准号:
    10624895
  • 负责人:
  • 金额:
    $ 112.04万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2021
  • 资助国家:
    美国
  • 起止时间:
    2021-08-01 至 2026-05-31
  • 项目状态:
    未结题

项目摘要

PROJECT SUMMARY While mice are essential models for many areas of neuroscience, there are also many aspects of higher brain function and dysfunction that cannot be adequately modeled in rodents. Thus, there is a need for new genetic models that have brain structure and function closer to humans. For these reasons, non-human primates (NHP) provide an attractive model to study higher brain function and brain disorders. A promising emerging NHP model is the common marmoset, a small New World primate that has many advantages as a genetic model. Although the adaptation of bacterial CRISPR/Cas systems for targeted genome engineering and model creation has revolutionized modern biology, editing of the marmoset genome is still in its infancy. Given the significant time and money required for marmoset genome editing, new methods to increase editing efficiency, decrease mosaicism, and identify correctly-edited embryos prior to transfer to recipient females are critical. Additionally, new methods for controlling the zygosity of founder animals are necessary to enable analysis of homozygous F0 animals and to avoid homozygous editing when targeting essential genes that cause embryonic lethality upon biallelic disruption. To these ends, we propose a research program that will significantly enhance our ability to introduce multiple types of edits into the marmoset genome, reduce mosaicism, control the zygosity of edits, and identify successfully edited embryos through prenatal genetic testing. We will disseminate these technologies and models through direct resource sharing, in-person trainings, deposition to NIH-supported Marmoset Coordination Center. Together, the proposed advances will significantly reduce the time, effort, costs and animal numbers necessary to marmoset genetic models and will unlock the true potential of marmosets for basic and translational neuroscience research.
项目总结

项目成果

期刊论文数量(0)
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会议论文数量(0)
专利数量(0)

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Guoping Feng其他文献

Guoping Feng的其他文献

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{{ truncateString('Guoping Feng', 18)}}的其他基金

BRAIN CONNECTS: Comprehensive regional projection map of marmoset with single axon and cell type resolution
大脑连接:具有单轴突和细胞类型分辨率的狨猴综合区域投影图
  • 批准号:
    10664170
  • 财政年份:
    2023
  • 资助金额:
    $ 112.04万
  • 项目类别:
Functional dissection of thalamocortical interactions through genetically-defined TRN subnetworks
通过基因定义的 TRN 子网络对丘脑皮质相互作用进行功能剖析
  • 批准号:
    10622039
  • 财政年份:
    2022
  • 资助金额:
    $ 112.04万
  • 项目类别:
Developing cell type-specific enhancers and connectivity mapping pipelines for marmosets
开发狨猴的细胞类型特异性增强剂和连接映射管道
  • 批准号:
    10653998
  • 财政年份:
    2021
  • 资助金额:
    $ 112.04万
  • 项目类别:
A Genetic Engineering Toolbox for Marmosets (GETMarm): Development and optimization of genome editing and assisted reproduction techniques for marmoset models
狨猴基因工程工具箱 (GETMarm):狨猴模型基因组编辑和辅助生殖技术的开发和优化
  • 批准号:
    10286437
  • 财政年份:
    2021
  • 资助金额:
    $ 112.04万
  • 项目类别:
A Genetic Engineering Toolbox for Marmosets (GETMarm): Development and optimization of genome editing and assisted reproduction techniques for marmoset models
狨猴基因工程工具箱 (GETMarm):狨猴模型基因组编辑和辅助生殖技术的开发和优化
  • 批准号:
    10459550
  • 财政年份:
    2021
  • 资助金额:
    $ 112.04万
  • 项目类别:
Developing cell type-specific enhancers and connectivity mapping pipelines for marmosets
开发狨猴的细胞类型特异性增强剂和连接映射管道
  • 批准号:
    10478105
  • 财政年份:
    2021
  • 资助金额:
    $ 112.04万
  • 项目类别:
A Genetic Engineering Toolbox for Marmosets (GETMarm): Development and optimization of genome editing and assisted reproduction techniques for marmoset models
狨猴基因工程工具箱 (GETMarm):狨猴模型基因组编辑和辅助生殖技术的开发和优化
  • 批准号:
    10832288
  • 财政年份:
    2021
  • 资助金额:
    $ 112.04万
  • 项目类别:
Developing cell type-specific enhancers and connectivity mapping pipelines for marmosets
开发狨猴的细胞类型特异性增强剂和连接映射管道
  • 批准号:
    10271630
  • 财政年份:
    2021
  • 资助金额:
    $ 112.04万
  • 项目类别:
Functional dissection of thalamocortical interactions through genetically-defined TRN subnetworks
通过基因定义的 TRN 子网络对丘脑皮质相互作用进行功能剖析
  • 批准号:
    10408808
  • 财政年份:
    2019
  • 资助金额:
    $ 112.04万
  • 项目类别:
Functional dissection of thalamocortical interactions through genetically-defined TRN subnetworks
通过基因定义的 TRN 子网络对丘脑皮质相互作用进行功能剖析
  • 批准号:
    10198061
  • 财政年份:
    2019
  • 资助金额:
    $ 112.04万
  • 项目类别:

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非洲人群中 HIV 氨基酸变异与 CHD1L 和 HLA I 类基因座的保护性宿主等位基因的关联
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