Characterization of the roles and regulation of Draxin in cranial neural crest
Draxin 在颅神经嵴中的作用和调节的表征
基本信息
- 批准号:10632334
- 负责人:
- 金额:$ 24.9万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-07-01 至 2025-06-30
- 项目状态:未结题
- 来源:
- 关键词:AffectBindingBiochemistryBioinformaticsBiological AssayCartilageCell AdhesionCell Adhesion MoleculesCell-Cell AdhesionCephalicChIP-seqChickCleft PalateCo-ImmunoprecipitationsCraniofacial AbnormalitiesDataDefectDevelopmentDiGeorge SyndromeDiseaseDown-RegulationE-CadherinEmbryoEmbryologyEtiologyEventExtracellular Matrix ProteinsFaceFluorescence Resonance Energy TransferGelatinase BGene ExpressionGenetic TranscriptionGenomic SegmentGoalsHeadImageImmunoprecipitationIn Situ HybridizationIntegrinsLamininLeadLigationLightMaintenanceMandibulofacial DysostosisMediatingMentorsMessenger RNAMicroscopyMolecularMutateMutationN-CadherinNamesNeural CrestNeural Crest CellNeural tubeNeuraxisPathway interactionsPhasePlayPopulationPost-Transcriptional RegulationProteinsRNARNA-Binding ProteinsRegulationReporterResearchRoleSiteSkeletonTailTechniquesTimeTranslational ResearchUntranslated RegionsUp-RegulationWNT Signaling Pathwayantagonistbeta catenincraniofacialcraniofacial structureepithelial to mesenchymal transitionexperimental studyin vivoknock-downlaminin alpha5mRNA Expressionmalformationmigrationneural plateneuroepitheliumoverexpressionpublic health relevancereceptorsensorspatiotemporalstem cell populationtime use
项目摘要
PROJECT SUMMARY
The neural crest (NC) is a stem cell population that originates within the forming central nervous
system. NC cells delaminate from the neuroepithelium by undergoing a spatiotemporally regulated epithelial-to-
mesenchymal transition (EMT) that proceeds in a coordinated wave head-to-tail to exit from the neural tube.
Cranial NC cells, which arise in the head region of the embryo and are the only NC population in vivo with the
ability to differentiate into craniofacial skeleton and cartilage, are indispensable for the development of the face;
mutations affecting NC development result in numerous diseases and malformations affecting the craniofacial
structures (e.g. Treacher Collins syndrome, cleft palate, etc.). Thus, a thorough understanding of the regulation
of cranial NC EMT is essential to identify the etiology of craniofacial abnormalities.
To date, research has focused on unraveling early events governing cranial NC induction at the neural
plate border and the mechanism of delamination from the neural tube. However, little is known about the
regulatory changes governing the timing of NC EMT and exit from the neural tube. My preliminary studies
demonstrate that a Wnt pathway antagonist, Draxin, plays an important role in controlling the timing of NC
EMT, perturbations of which have negative consequences for cranial NC migration. This role for Draxin is
particularly interesting in light of the fact that the Wnt signaling pathway has been shown to be a major driver of
NC development. The goal of this Proposal is to investigate the molecular mechanism by which Draxin affects
cranial NC EMT, with focus on its role in Wnt signaling. To this end, the Specific Aims of this Proposal seek to
combine chick embryology with state-of-the-art imaging and biochemistry to:
1) Characterize the interaction of Draxin and Wnt signaling in cranial NC EMT;
2) Identify the role of Draxin in the regulation of cell adhesion proteins;
3) Characterize the post-transcriptional regulation of Draxin; and
4) Identify the role of Draxin in the regulation of extracellular matrix proteins.
The results of this Proposal will significantly enhance our understanding of the regulation of cranial NC
EMT and migration, providing new scientific avenues for translational research applications in the treatment of
craniofacial defects.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Erica Hutchins其他文献
Erica Hutchins的其他文献
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{{ truncateString('Erica Hutchins', 18)}}的其他基金
Mechanisms of RNA turnover during the epithelial-mesenchymal transition
上皮-间质转化过程中 RNA 周转的机制
- 批准号:
10714965 - 财政年份:2023
- 资助金额:
$ 24.9万 - 项目类别:
Characterization of the roles and regulation of Draxin in cranial neural crest
Draxin 在颅神经嵴中的作用和调节的表征
- 批准号:
10653270 - 财政年份:2022
- 资助金额:
$ 24.9万 - 项目类别:
Characterization of the roles and regulation of Draxin in cranial neural crest
Draxin 在颅神经嵴中的作用和调节的表征
- 批准号:
10730206 - 财政年份:2022
- 资助金额:
$ 24.9万 - 项目类别:
Characterization of the roles and regulation of Draxin in cranial neural crest
Draxin 在颅神经嵴中的作用和调节的表征
- 批准号:
10843333 - 财政年份:2022
- 资助金额:
$ 24.9万 - 项目类别:
Characterization of the roles and regulation of Draxin in cranial neural crest
Draxin 在颅神经嵴中的作用和调节的表征
- 批准号:
10400365 - 财政年份:2021
- 资助金额:
$ 24.9万 - 项目类别:
Functional analysis of draxin in cranial neural crest emigration
draxin在颅神经嵴移出中的功能分析
- 批准号:
9391932 - 财政年份:2016
- 资助金额:
$ 24.9万 - 项目类别:
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