Mechanisms of Basal Forebrain Control Over Sensory Processing

基底前脑控制感觉处理的机制

• 批准号: 10634890
• 负责人: Elizabeth Hanson Moss
• 金额: $ 5.4万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10634890
• 关键词: Animals; Arousal; Attention; Award; Behavior; Behavioral; Breeding; Cognitive deficits; Collaborations; Complex; Data Analyses; Data Collection; Data Storage and Retrieval; Electrophysiology (science); Fiber; Future; Genetic Engineering; Goals; Maternity leave; Mus; Nature; Neurodegenerative Disorders; Operative Surgical Procedures; Output; Parents; Photometry; Preparation; Productivity; Reagent; Recovery; Research; Resources; Rewards; Role; Schedule; Secure; Sensory; Signal Transduction; Slice; Testing; Time; TimeLine; Training; Transgenic Mice; Work; basal forebrain; cholinergic; computerized data processing; experimental study; flexibility; in vivo two-photon imaging; mouse model; neural circuit; olfactory bulb; virus genetics

PROJECT SUMMARY The overarching goals of the parent award are to develop a mechanistic understanding of how neural circuits in the basal forebrain impact sensory processing. Toward this, the proposed Aims in the parent award test the hypothesis that effects of attention, arousal, and reward prediction on olfactory processing rely on precisely timed, cholinergic signaling within the basal forebrain controlling projection output to the olfactory bulb. This hypothesis is being tested using a combination of viral genetic engineering, transgenic mouse models, in vivo two-photon imaging, fiber photometry, mouse behavior, and slice electrophysiology. These complex experimental paradigms have relatively long timelines for generating mice, performing surgeries, recovery, training, and data collection. Pausing experimentation for maternity leave would not only disrupt ongoing experiments, but it would seriously impact future experiments requiring the breeding, preparation, and training of mice. This supplement proposal seeks to avoid a significant disruption in continuity during the maternity leave of the parent award PI by providing for (1) a part-time technician for nine months and (2) data storage server hardware allowing secure, remote access and collaboration across multiple users. The role of the technician will first be to maintain ongoing mouse experimentation as the PI is fully on leave. Then, as the PI returns from leave part time, the role of the technician will shift to a combination of maintaining experiments, processing data, and preparing mice for future slice electrophysiology experiments. Finally, as the PI returns to work full time the role of the technician will be to maintain the continuity of behavioral experimentation in the case that flexible scheduling is required on the part of the PI due to the unpredictable nature of childcare. At the same time, implementing a remotely accessible data storage server will enable real time collaboration between the technician and the PI for the duration of leave and after returning to work full time. Together these resources will help maximize productivity and promote continuity of the parent award during the maternity leave of the parent award PI.

项目摘要 家长奖的首要目标是发展一个机械的理解，神经回路如何在 基底前脑影响感觉处理。为此，在父母奖的拟议目标测试， 假设注意力、唤醒和奖励预测对嗅觉处理的影响依赖于 基底前脑内控制投射输出到嗅球的定时胆碱能信号。这 目前正在使用病毒基因工程、转基因小鼠模型、体内 双光子成像、纤维光度测定、小鼠行为和切片电生理学。这些复杂 实验范例对于产生小鼠、进行手术、恢复 培训和数据收集。暂停产假的实验不仅会破坏正在进行的 实验，但它会严重影响未来的实验，需要育种，准备和培训， 对小鼠这一补充建议旨在避免产假期间的连续性受到重大干扰 通过提供（1）九个月的兼职技术人员和（2）数据存储服务器， 支持多个用户之间的安全远程访问和协作的硬件。技术人员的作用将 首先是保持正在进行的小鼠实验，因为PI完全休假。当私家侦探休假回来时 兼职，技术人员的角色将转移到维护实验，处理数据， 为将来的切片电生理学实验准备小鼠。最后，当PI返回全职工作时， 技术人员的任务是在灵活的情况下保持行为实验的连续性， 由于儿童保育的不可预测性，需要PI进行安排。与此同时， 实施远程可访问数据存储服务器将实现 技术员和PI在休假期间和返回全职工作岗位后。这些资源将 帮助最大限度地提高生产力，并促进父母产假期间父母补助金的连续性 奖励PI。