Metabolic basis of mosquito-endosymbiont-virus interactions RBL Admin Supplement

蚊子-内共生体-病毒相互作用的代谢基础 RBL 管理补充品

• 批准号: 10631510
• 负责人: Rushika Perera
• 金额: $ 37.01万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10631510
• 关键词: Acute; Administrative Supplement; Aedes; Arboviruses; Award; Bacteria; Biocontrols; Biophysics; Blood Vessels; Cell Culture Techniques; Cells; Chikungunya virus; Choking; Country; Culicidae; Data; Dengue Fever; Dengue Hemorrhagic Fever; Dengue Shock Syndrome; Dengue Virus; Diagnostic; Energy Metabolism; Extravasation; Family; Fever; Flaviviridae; Funding; Genome; Goals; Grant; Heritability; Human; In Vitro; Infection; Insecta; Investigation; Laboratories; Life; Life Cycle Stages; Lipids; Lipolysis; Maintenance; Mediating; Membrane; Metabolic; Metabolic Pathway; Methods; Modeling; Modification; National Institute of Allergy and Infectious Disease; Outcome; Parents; Pathway interactions; Persons; Phenotype; RNA Viruses; Research; Resources; Risk; Serotyping; Site; Sphingolipids; Structure; Syndrome; Therapeutic; Tissues; Translations; United States National Institutes of Health; Vaccinee; Vaccines; Viral; Viral Physiology; Viral Proteins; Virion; Virus; Virus Replication; Wolbachia; World Health Organization; Zika Virus; antagonist; biophysical analysis; chikungunya infection; density; endosymbiont; fitness; insight; interest; lipid biosynthesis; metabolic phenotype; metabolome; mitochondrial metabolism; particle; pathogen; success; symbiont; transmission process; vector; vector mosquito; viral transmission

PROJECT SUMMARY / ABSTRACT This supplement is responsive to the Notice of Special Interest, NOT-AI-22-049, “Administrative Supplements for NIAID Regional Biocontainment Laboratories (RBL) and other NIH Grantees Conducting Research at RBL- affiliated Sites” and the scope of our NIAID grant, (fund R01AI151166), entitled, “Metabolic basis of mosquito- endosymbiont-virus interactions.” Here, we will accelerate and advance Specific Aims 1-3 of the parent award through two primary supplementary aims; SA 1-3.supp1: Assess how modification of cellular lipids alters the composition of the viral envelope and required structural transitions for maturation and infectivity in Ae. Aegypti. The data generated from SA 1-3.supp1 will significantly enhance the parent award data outcomes as it provides biophysical insight into how lipid modulation might alter virus structure and function and thus impact the viral life cycle in Ae. aegypti. SA 2.supp2: Assess how Wolbachia-mediated lipid changes alter ZIKV and CHIKV infection and virus-associated lipid modulations. The data generated from SA 2.supp2 will significantly enhance the parent award data outcomes by providing additional insight into the metabolic state of the vector following infection with all three vectored viruses. The studies will enhance our understanding of metabolic choke points that may be applicable to all three viruses, and/or significant differences that might hinder biocontrol efforts. Both efforts synergize with the goals of the RBL in (diagnostics, therapeutics, vaccines, and transmission control methods for Arboviruses vectored by Ae. aegypti); and has the full support of the Scientific Director of the RMRBL.

项目摘要 /摘要 这种补充对特殊关注的通知有回应，而不是-AI-22-049，“行政补品 用于NIAID区域生物疗养实验室（RBL）和其他NIH授予者，在RBL-进行研究 会员站点”和我们的NIAID赠款的范围（基金R01AI151166），题为“蚊子的代谢基础 内共生病毒相互作用。 通过两个主要的补充目标； SA 1-3.SUPP1：评估细胞脂质的修饰如何改变 病毒包膜的组成和AE成熟和感染所需的结构过渡。埃及。 SA 1-33产生的数据将显着增强父母奖励数据结果 关于脂质调节如何改变病毒结构和功能，从而影响病毒寿命的生物物理洞察力 在AE中循环。埃及。 SA 2.Supp2：评估Wolbachia介导的脂质变化如何改变ZIKV和CHIKV感染 和与病毒相关的脂质调制。 SA 2.SUPP2产生的数据将显着增强父 通过在感染后对矢量的代谢状态提供更多见解，奖励数据结果 所有三个载体病毒。这些研究将增强我们对可能是代谢扼流点的理解 适用于所有三种病毒和/或可能阻碍生物防治努力的显着差异。两者都努力 与RBL的目标协同作用（诊断，治疗，疫苗和传输控制方法 对于由AE载体的Arbovirus。埃及）;并得到了RMRBL科学总监的全力支持。