Metabolic basis of mosquito-endosymbiont-virus interactions RBL Admin Supplement

蚊子-内共生体-病毒相互作用的代谢基础 RBL 管理补充品

• 批准号: 10631510
• 负责人: Rushika Perera
• 金额: $ 37.01万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10631510
• 关键词: Acute; Administrative Supplement; Aedes; Arboviruses; Award; Bacteria; Biocontrols; Biophysics; Blood Vessels; Cell Culture Techniques; Cells; Chikungunya virus; Choking; Country; Culicidae; Data; Dengue Fever; Dengue Hemorrhagic Fever; Dengue Shock Syndrome; Dengue Virus; Diagnostic; Energy Metabolism; Extravasation; Family; Fever; Flaviviridae; Funding; Genome; Goals; Grant; Heritability; Human; In Vitro; Infection; Insecta; Investigation; Laboratories; Life; Life Cycle Stages; Lipids; Lipolysis; Maintenance; Mediating; Membrane; Metabolic; Metabolic Pathway; Methods; Modeling; Modification; National Institute of Allergy and Infectious Disease; Outcome; Parents; Pathway interactions; Persons; Phenotype; RNA Viruses; Research; Resources; Risk; Serotyping; Site; Sphingolipids; Structure; Syndrome; Therapeutic; Tissues; Translations; United States National Institutes of Health; Vaccinee; Vaccines; Viral; Viral Physiology; Viral Proteins; Virion; Virus; Virus Replication; Wolbachia; World Health Organization; Zika Virus; antagonist; biophysical analysis; chikungunya infection; density; endosymbiont; fitness; insight; interest; lipid biosynthesis; metabolic phenotype; metabolome; mitochondrial metabolism; particle; pathogen; success; symbiont; transmission process; vector; vector mosquito; viral transmission

PROJECT SUMMARY / ABSTRACT This supplement is responsive to the Notice of Special Interest, NOT-AI-22-049, “Administrative Supplements for NIAID Regional Biocontainment Laboratories (RBL) and other NIH Grantees Conducting Research at RBL- affiliated Sites” and the scope of our NIAID grant, (fund R01AI151166), entitled, “Metabolic basis of mosquito- endosymbiont-virus interactions.” Here, we will accelerate and advance Specific Aims 1-3 of the parent award through two primary supplementary aims; SA 1-3.supp1: Assess how modification of cellular lipids alters the composition of the viral envelope and required structural transitions for maturation and infectivity in Ae. Aegypti. The data generated from SA 1-3.supp1 will significantly enhance the parent award data outcomes as it provides biophysical insight into how lipid modulation might alter virus structure and function and thus impact the viral life cycle in Ae. aegypti. SA 2.supp2: Assess how Wolbachia-mediated lipid changes alter ZIKV and CHIKV infection and virus-associated lipid modulations. The data generated from SA 2.supp2 will significantly enhance the parent award data outcomes by providing additional insight into the metabolic state of the vector following infection with all three vectored viruses. The studies will enhance our understanding of metabolic choke points that may be applicable to all three viruses, and/or significant differences that might hinder biocontrol efforts. Both efforts synergize with the goals of the RBL in (diagnostics, therapeutics, vaccines, and transmission control methods for Arboviruses vectored by Ae. aegypti); and has the full support of the Scientific Director of the RMRBL.

项目总结/摘要 本补充材料是对特别关注通知（NOT-AI-22-049）“管理补充材料”的回应 为NIAID区域生物防护实验室（RBL）和其他NIH赠款人在RBL进行研究- 附属网站”和我们的NIAID赠款的范围，（基金R 01 AI 151166），题为“蚊子的代谢基础- 内共生体与病毒的相互作用”在这里，我们将加速和推进父奖的具体目标1-3 通过两个主要的补充目标; SA 1-3.supp1：评估细胞脂质的修饰如何改变细胞的功能。 组成的病毒包膜和所需的结构转变的成熟和感染性在Ae。埃及人 SA 1-3.supp1生成的数据将显著增强母公司奖励数据结果，因为它提供 脂质调节如何改变病毒结构和功能，从而影响病毒生命的生物物理学见解 循环在Ae。埃及人。SA 2.supp2：评估Wolbachia介导的脂质变化如何改变ZIKV和CHIKV感染 和病毒相关的脂质调节。从SA 2.supp2生成的数据将显著增强父 通过提供对感染后载体代谢状态的额外洞察来奖励数据结果 所有三种载体病毒这些研究将增强我们对代谢瓶颈的理解， 适用于所有三种病毒，和/或可能阻碍生物防治工作的显著差异。这两项努力 在（诊断、治疗、疫苗和传播控制方法）方面与RBL的目标协同 Ae携带的虫媒病毒。埃及）;并得到了RMRBL科学主任的全力支持。