Core Vision Research Grant

核心愿景研究补助金

• 批准号: 10630454
• 负责人: PAUL STEVEN BERNSTEIN
• 金额: $ 15.13万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630454
• 关键词: Animal Model; Anterior; Area; Award; Basic Science; Biochemistry; Cellular biology; Chemistry; Collaborations; Cryoultramicrotomy; Databases; Dimensions; Disease; Electrophysiology (science); Evolution; Eye; Faculty; Gel; Genes; Genotype; Glaucoma; Grant; High Pressure Liquid Chromatography; Human Genetics; Image; Imaging Device; In Situ Hybridization; In Vitro; Individual; Knowledge; Laboratories; Ligands; Link; Mass Fragmentography; Mathematics; Molecular; Molecular Biology; Mus; Neurobiology; Neurosciences; Optics; Physiology; Preparative Centrifugation; Proteins; Proteomics; Research; Research Personnel; Research Project Grants; Resources; Retina; Retinal Degeneration; Scanning; Schools; Science of genetics; Services; Silicon; Software Tools; Surface Plasmon Resonance; System; Technical Expertise; Technology; Tissues; Training; Translational Research; Ultracentrifugation; Universities; Utah; Validation; Vision research; Western Blotting; Yang; base; behavior test; computer science; confocal imaging; design; developmental disease; eye center; immunocytochemistry; in vivo; metabolomics resource; mouse genetics; ocular imaging; tool; two-photon; working group

Project Summary The OVERALL aims of this Vision Research Core (VRC) are to provide: • access to resources outside the scope of individual PIs and their R01 awards • access to technical expertise outside the scope a single laboratory • staff training to remove barriers to efficient translational research and collaboration • collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Imaging Module provides a range of imaging (TEM, confocal, scanning optical) and computing services (imaging, database, mathematics) based on strengths of core laboratories and the tradition of excellence of the UU School of Computing, whose descendants founded Adobe Systems, Silicon Graphics, Netscape and Pixar. Collaborations among these groups have transformed software tools for TEM and confocal imaging. The Cell Biology Module derives from the strengths in mouse genetics at the Moran Eye Center and the high demand for tissue profiling. Almost every JMEC laboratory has need of high quality cryosectioning, paratoming, mouse genotyping, immunostaining training, immunocytochemistry, quantitative PCR, in-situ hybridization technologies. . The Physiology Module is a powerful set of tools that VRC faculty use for animal model validation and disease profiling: ERG set-ups (LKC Big Shot and an older LKC setup), multi-focal ERG (Phoenix), in vivo / in vitro 2-photon imaging, OptoMotry and Rotarod behavioral testing, and a range of ocular imaging tools (OCT, Micron). The Biochemistry Module evolved from a gene sequencing/primer synthesis service towards a true proteomics more accurately reflecting our current research strengths (ultracentrifugation, preparative centrifugation, Odyssey quantitative immunoblotting, gel quantitation) and metabolomics resource (specialized GC-MS, HPLC-MS, surface plasmon resonance and protein-ligand interactions). The Administrative Module provides professional grants management for the VRC.

项目摘要 该愿景研究核心（VRC）的总体目标是提供： •访问个人PI及其R01奖励范围之外的资源 •范围外的技术专长访问一个实验室 •员工培训以消除有效的转化研究和协作的障碍 •VRC实验室的合作计划 VRC支持的研究领域跨越了残余变性的分析和处理， 发育障碍，青光眼和其他疾病以及一系列尖端的基础科学 倡议。我们已经实施了四个资源模块，这些模块继续了这种方式的自然演变 研究小组共同合作，为17名持有22个NEI R01奖项的调查人员提供服务。 成像模块提供一系列成像（TEM，共聚焦，扫描光学）和计算服务 （成像，数据库，数学）基于核心实验室的优势和卓越的传统 UU计算学院的后代创建了Adobe系统，硅图形，Netscape和Pixar。 这些小组之间的协作改变了用于TEM和共聚焦成像的软件工具。 细胞生物学模块来自Moran眼中的小鼠遗传学的强度和高 对组织分析的需求。几乎每个JMEC实验室都需要高质量的冷冻效果， 角化，小鼠基因分型，免疫染色训练，免疫细胞化学，定量PCR，原位 杂交技术。 。 生理模块是一组强大的工具，用于VRC教师用于动物模型验证和 疾病分析：ERG设置（LKC Big Shot和较旧的LKC设置），多焦点ERG（凤凰），体内 / IN 体外2光子成像，验光和旋转行为测试以及一系列眼部成像工具（OCT， 微米）。 生物化学模块从基因测序/引物合成服务演变为真实 蛋白质组学更准确地反映我们当前的研究强度（超离心，制备了 离心，奥德赛定量免疫印迹，凝胶定量）和代谢组学资源（专业 GC-MS，HPLC-MS，表面等离子体共振和蛋白质 - 配体相互作用）。 行政模块为VRC提供了专业的赠款管理。