Biochemistry

生物化学

• 批准号: 10669736
• 负责人: PAUL STEVEN BERNSTEIN
• 金额: $ 15.69万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10669736
• 关键词: Antibodies; Area; Award; Basic Science; Biochemical; Biochemistry; Biosensor; Carotenoids; Cells; Cholesterol; Collaborations; DNA; Detection; Disease; Electroporation; Evolution; Faculty; Fatty Acids; Flavonoids; Gel; Genes; Glaucoma; High Pressure Liquid Chromatography; Image; Individual; Laboratories; Ligands; Lipids; Lipofuscin; Mass Fragmentography; Molecular Biology; Neonatal; Nucleic Acids; Oligonucleotides; Organelles; Photoreceptors; Plasmids; Protein Analysis; Proteins; Proteomics; Radioactivity; Research; Research Personnel; Research Project Grants; Resources; Retinal Degeneration; Retinoids; Services; Shuttle Vectors; Sucrose; Surface Plasmon Resonance; Technical Expertise; Technology; Training; Transfection; Translational Research; Ultracentrifugation; Universities; Utah; Vision research; Western Blotting; X-Ray Film; archive data; data archive; developmental disease; in vivo; instrumentation; interest; intermolecular interaction; metabolomics resource; plasmid DNA; protein expression; small molecule; working group

Project Summary-Biochemistry Core The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration · collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Biochemistry Module evolved from an oligonucleotide synthesis and gene sequencing service towards a true proteomics (ultracentrifugation, gel quantitation, immunoblotting) and metabolomics resource (specialized GC-MS, HPLC-MS, surface plasmon resonance) that accurately reflects our current research strengths. Specifically, the Biochemistry Module provides: · Ultracentrifugation based on a workhorse Beckman-Coulter XL-90 · IR imaging and quantitation of immunoblots and hybridization blots · Quantitative Western blotting · X-ray film developing for ECL and radioactivity detection · Neonatal in vivo electroporation of DNA expression constructs into cells · HPLC-MS and GC-MS of small molecules (retinoids, carotenoids, lipofuscin components, fatty acids, cholesterol, flavonoids) · Protein/ligand interaction quantitation with SPR biosensor technologies · Training and comprehensive data archiving.

项目摘要生物化学核心 该愿景研究核心（VRC）的总体目标是提供： ·访问个人R01奖项范围之外的资源 ·范围外的技术专长访问一个实验室 员工培训以消除有效的转化研究和协作的障碍 VRC实验室之间的合作计划 VRC支持的研究领域跨越了残余变性的分析和处理， 发育障碍，青光眼和其他疾病以及一系列尖端的基础科学 倡议。我们已经实施了四个资源模块，这些模块继续了这种方式的自然演变 研究小组共同合作，为17名持有22个NEI R01奖项的调查人员提供服务。 生物化学模块从寡核苷酸的合成和基因测序服务演变为 真正的蛋白质组学（超速离心，凝胶定量，免疫印迹）和代谢组学资源（专业 GC-MS，HPLC-MS，表面等离子体共振），可以准确反映我们当前的研究强度。 具体而言，生物化学模块提供： 基于beckman-coulter XL-90的超速离心 ·免疫印迹和杂交块的红外成像和定量 定量蛋白质印迹 X射线膜开发用于ECL和放射性检测 ·DNA表达构建体的新生儿在体内电穿孔中 小分子的HPLC-MS和GC-MS（类视黄素，类胡萝卜素，脂肪霉素成分，脂肪 酸，胆固醇，类黄酮） ·与SPR生物传感器技术的蛋白质/配体相互作用定量 ·培训和全面数据归档。