Biochemistry

项目摘要-生物化学核心 该愿景研究核心(VRC)的总体目标是提供： ·获得个人R01奖励范围以外的资源 ·获得单一实验室范围以外的技术专长 ·员工培训，以消除高效翻译研究和协作的障碍 ·VRC实验室之间的协作计划 VRC支持的研究领域涵盖视网膜变性的分析和治疗， 发育障碍、青光眼和其他障碍，以及一系列尖端基础科学 首创精神。我们已经实现了四个资源模块，它们继续自然演变为 研究小组通力合作，为17名研究人员提供服务，获得了22个NEI R01奖。 生物化学模块从寡核苷酸合成和基因测序服务演变为 真正的蛋白质组学(超速离心、凝胶定量、免疫印迹)和代谢组学资源(专门的 GC-MS、LC-MS、表面等离子体共振)，准确地反映了我们目前的研究优势。 具体地说，生化模块提供： ·基于Beckman-Coulter XL-90的超速离心机 ·免疫印迹和杂交印迹的红外成像和定量 ·定量Western blotting ·用于ECL和放射性检测的X光胶片 ·新生儿体内DNA表达构建体电穿孔进入细胞 ·小分子(维甲酸、类胡萝卜素、脂褐素成分、脂肪 酸、胆固醇、类黄酮类) ·利用SPR生物传感器技术进行蛋白质/配体相互作用的定量 ·培训和全面的数据归档。

Project Summary-Biochemistry Core The OVERALL aims of this Vision Research Core (VRC) are to provide: · access to resources outside the scope of individual R01 awards · access to technical expertise outside the scope a single laboratory · staff training to remove barriers to efficient translational research and collaboration · collaboration initiatives among VRC labs The research areas supported by the VRC span the analysis and treatments of retinal degenerations, developmental disorders, glaucoma and other disorders, as well as and a range of cutting-edge basic science initiatives. We have implemented four resource modules that continue the natural evolution of how this research group works together, serving 17 investigators holding 22 NEI R01 awards. The Biochemistry Module evolved from an oligonucleotide synthesis and gene sequencing service towards a true proteomics (ultracentrifugation, gel quantitation, immunoblotting) and metabolomics resource (specialized GC-MS, HPLC-MS, surface plasmon resonance) that accurately reflects our current research strengths. Specifically, the Biochemistry Module provides: · Ultracentrifugation based on a workhorse Beckman-Coulter XL-90 · IR imaging and quantitation of immunoblots and hybridization blots · Quantitative Western blotting · X-ray film developing for ECL and radioactivity detection · Neonatal in vivo electroporation of DNA expression constructs into cells · HPLC-MS and GC-MS of small molecules (retinoids, carotenoids, lipofuscin components, fatty acids, cholesterol, flavonoids) · Protein/ligand interaction quantitation with SPR biosensor technologies · Training and comprehensive data archiving.