Core A: Ultrastructural Assessment & Human Tissue

摘要/摘要--科学核心结构 超微结构评估和人体组织核心的目标是最大限度地促进科学进步和 通过提供基本服务，提高PPG调查人员的工作效率。核心A的主要功能是服务于 所有项目和核心B都需要人和猪的心肌和星状神经节组织。 获取和研究人类心脏和星状神经节需要显著的监管和后勤障碍 对于纸巾来说，这是由一个中央核心处理的理想选择。核心将执行以下三个关键功能 研究：1)组织清除和超微结构成像；2)获取正常(或接近正常)和病变的标本 人体组织，特别是心脏和星状神经节；以及3)分子生物学。这个PPG的所有3个项目都有 使用这些服务中的至少两项，此核心和核心B之间将有紧密的联系，即功能 评估和工具核心。在过去的15个月里，我们修建了一条管道来获取疾病 来自接受原位心脏移植患者的心脏，以及正常或接近正常的心脏因 器官移植。我们从心肌病患者体内获得星状神经节的基础设施 非心肺疾病患者心肺时切除星状神经节和星状神经节 捐赠已经存在5年了。考虑到这些组织可获得的不可预测的时间， 将纸巾采购活动集中在这一核心范围内，将使私营部门司受益，因为它简化了程序和 审批、最大限度地降低成本和提高工作效率。核心还将简化为期4周的 通过分批组织从猪和人身上清除组织为了最大限度地减少错误，组织清除涉及到许多 步骤和化学物质。最后，最理想的是近距离高分辨率的组织清晰度和成像。 与本核心项目1-3的研究团队成员协调，无论组织是否被清理。 在PPG期间，核心是准备好的-拥有经验、强大的协议和经验丰富的人员 -提供关键服务，确保PPG的成功。

SUMMARY/ABSTRACT – Scientific Core - Structure The goal of the Ultrastructural Assessment and Human Tissue Core is to maximize the scientific progress and productivity of the PPG investigators by providing essential services. The primary function of Core A is to serve human and porcine myocardial and stellate ganglion tissue needs across all projects and Core B. Given significant regulatory and logistical hurdles needed to procure and study human heart and stellate ganglion tissues, it is ideal for this to be handled by a central core. The core will perform three key functions for the study: 1) tissue clearing and ultrastructural imaging; 2) procurement of normal (or near normal) and diseased human tissues, specifically hearts and stellate ganglia; and 3) molecular biology. All 3 projects of this PPG with utilize at least two of these services, and there will be tight links between this core and Core B, the Functional Assessment and Instrumentation Core. Over the past 15 months, we have built a pipeline to obtain diseased hearts from patients undergoing orthotopic heart transplantation, and normal or near normal hearts rejected for organ transplant. Our infrastructure for obtained stellate ganglia from patients with cardiomyopathy undergoing stellectomy, and stellate ganglia from patients without cardiopulmonary disease at the time of heart and/or lung donation has existed for 5 years. Given the unpredictable times at which these tissues become available, centralizing the tissue procurement activities within this core will benefit the PPG by streamlining protocols and approvals, minimizing cost, and boosting productivity. The Core will also streamline the 4-week process of tissue clearing from pigs and humans by batching tissues to minimize error, tissue clearing involves numerous steps and chemicals. Finally, it is most ideal that tissues are cleared and imaged in high resolution in close coordination with study team members from Projects 1-3 in this Core whether or not the tissues are cleared. For the duration of the PPG, the core is poised – with experience, robust protocols, and experienced personnel – to provide critical services that ensure the success of this PPG.