Core A: Ultrastructural Assessment & Human Tissue

核心 A：超微结构评估

• 批准号: 10627575
• 负责人: Olujimi A Ajijola
• 金额: $ 33.97万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-10 至 2028-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627575
• 关键词: 3-Dimensional; Achievement; Adrenergic Agents; Biological; Cardiac; Cardiomyopathies; Chemicals; Cicatrix; Data Set; Ensure; Family suidae; Functional disorder; Goals; Heart; Heart Diseases; Heart Transplantation; Human; Human Resources; Image; Infrastructure; Ion Channel; Lasers; Link; Lung; Maps; Methods; Molecular; Molecular Biology; Myocardial; Organ Procurements; Organ Transplantation; Patients; Penetration; Process; Productivity; Protocols documentation; Pulmonary Heart Disease; Recovery; Research Personnel; Resolution; Services; Structure; Structure of stellate ganglion; Sudden Death; Techniques; Testing; Therapeutic; Time; Tissue Procurements; Tissues; Validation; cell type; cost; experience; high resolution imaging; human disease; human tissue; innovation; instrumentation; member; mortality risk; neural; neuroregulation; neurotransmission; success; transcriptomics

SUMMARY/ABSTRACT – Scientific Core - Structure The goal of the Ultrastructural Assessment and Human Tissue Core is to maximize the scientific progress and productivity of the PPG investigators by providing essential services. The primary function of Core A is to serve human and porcine myocardial and stellate ganglion tissue needs across all projects and Core B. Given significant regulatory and logistical hurdles needed to procure and study human heart and stellate ganglion tissues, it is ideal for this to be handled by a central core. The core will perform three key functions for the study: 1) tissue clearing and ultrastructural imaging; 2) procurement of normal (or near normal) and diseased human tissues, specifically hearts and stellate ganglia; and 3) molecular biology. All 3 projects of this PPG with utilize at least two of these services, and there will be tight links between this core and Core B, the Functional Assessment and Instrumentation Core. Over the past 15 months, we have built a pipeline to obtain diseased hearts from patients undergoing orthotopic heart transplantation, and normal or near normal hearts rejected for organ transplant. Our infrastructure for obtained stellate ganglia from patients with cardiomyopathy undergoing stellectomy, and stellate ganglia from patients without cardiopulmonary disease at the time of heart and/or lung donation has existed for 5 years. Given the unpredictable times at which these tissues become available, centralizing the tissue procurement activities within this core will benefit the PPG by streamlining protocols and approvals, minimizing cost, and boosting productivity. The Core will also streamline the 4-week process of tissue clearing from pigs and humans by batching tissues to minimize error, tissue clearing involves numerous steps and chemicals. Finally, it is most ideal that tissues are cleared and imaged in high resolution in close coordination with study team members from Projects 1-3 in this Core whether or not the tissues are cleared. For the duration of the PPG, the core is poised – with experience, robust protocols, and experienced personnel – to provide critical services that ensure the success of this PPG.

概要/摘要-科学核心-结构 超微结构评估和人体组织核心的目标是最大限度地提高科学进步， 通过提供基本服务提高PPG调查人员的生产力。核心A的主要功能是 所有项目和核心B中的人和猪心肌和星状神经节组织需求。给定 获取和研究人类心脏和星状神经节需要重大的监管和后勤障碍 组织，这是理想的，这是由中央核心处理。该核心将执行三个关键功能， 研究：1）组织清除和超微结构成像; 2）正常（或接近正常）和病变的采购 人体组织，特别是心脏和星状神经节;和3）分子生物学。本PPG的所有3个项目， 至少使用其中两项服务，并且在此核心与核心B（功能性 评估和仪器核心。在过去的15个月里，我们建立了一个管道， 来自接受原位心脏移植的患者的心脏，以及正常或接近正常的心脏， 器官移植我们的基础设施获得星状神经节从心肌病患者接受 心脏和/或肺切除时无心肺疾病的患者的胸骨切开术和星状神经节 捐赠已经存在了5年。考虑到这些组织变得可用的不可预测的时间， 将组织采购活动集中在该核心内将通过简化协议而使PPG受益， 审批，最大限度地降低成本，提高生产力。核心还将简化为期4周的 为了使误差最小化，组织清除涉及许多 步骤和化学品。最后，最理想的是在近距离内以高分辨率对组织进行清除和成像。 与本核心中项目1-3的研究团队成员协调，无论组织是否被清除。 在PPG期间，核心团队已做好准备-拥有丰富的经验、稳健的方案和经验丰富的人员 - 提供关键服务，确保PPG的成功。