Human Tissue and Cell Core

人体组织和细胞核心

• 批准号: 10627595
• 负责人: Ivana V Yang
• 金额: $ 22.74万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-07-01 至 2028-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10627595
• 关键词: Aliquot; Area; Biological; Biopsy; Bronchiectasis; Cell Culture Techniques; Cell Separation; Cells; Classification; Clinical; Cryopreservation; Cryopreserved Cell; Data; Dissection; Distal; Drug or chemical Tissue Distribution; Epithelial Cells; Epithelium; Fibroblasts; Fibrosis; Genotype; Goals; Histopathology; Image; Injury; Investigation; Location; Lung; MUC5B gene; Participant; Pathologic; Pathologist; Phenotype; Process; Pulmonary Fibrosis; Quality Control; Radiology Specialty; Research; Resolution; Resources; Role; Sample Size; Scanning; Specimen; Tissue Banks; Tissues; Traction; X-Ray Computed Tomography; airway epithelium; alveolar epithelium; cell type; clinical phenotype; cohort; experimental study; human tissue; lung injury; phenotypic data; programs; recruit; risk variant

ABSTRACT The overall goal of this Program is to understand the role of MUC5B in establishing a vulnerable lung and the transition of a vulnerable lung to a lung characterized by persistent injury of bronchoalveolar epithelia and activation of lung fibroblasts. Core B: Human Tissue and Cell Core will as serve as a centralized location for resources needed for human tissue- and cell-based investigations in the Projects and is therefore an integral integrative component of the Program. Human tissue and cells isolated from specific functional lung regions of extensively phenotyped IPF research participants are a unique resource that will benefit all three Projects in the Program and will need to be carefully maintained. The goals of Core B, the Human Tissue and Cell Core, are to recruit IPF subjects and phenotype their clinical, radiologic and pathologic features; obtain, process, genotype and store tissue specimens of IPF subjects and controls; and to make available to each Project in this Program high quality tissues and cells from specific regions of the fibrotic and normal lung. We will do this via the following key functions: 1. Recruitment of IPF subjects and controls 2. Clinical phenotyping 3. Specimen acquisition, genotyping, banking and distribution 4. Isolation and storage of unpassaged and unselected cells (all cell types) from different functional regions of IPF and control lungs. 5. Isolation and storage of airway epithelial cells, alveolar epithelial cells and fibroblasts from different functional regions of IPF and control lungs. 6. Distribution of tissue, cells, and clinical phenotyping data to Projects. The Human Tissue and Cell Core will serve as a centralized location for resources needed for human tissue- and cell-based investigations in the Projects and is therefore an integral integrative component of the Program. Human tissue and cells isolated from specific functional lung regions of extensively phenotyped IPF research participants are a unique resource that will benefit all three Projects in the Program and will need to be carefully maintained.

摘要 该项目的总体目标是了解MUC 5 B在建立脆弱肺中的作用， 脆弱肺转变为以支气管肺泡上皮细胞持续损伤为特征的肺， 肺成纤维细胞的活化。核心B：人体组织和细胞核心将作为集中位置， 项目中基于人体组织和细胞的研究所需的资源，因此是不可或缺的 方案的组成部分。从特定功能性肺区域分离的人组织和细胞， 广泛的表型IPF研究参与者是一种独特的资源，将使所有三个项目受益， 计划和将需要仔细维护。 核心B（人体组织和细胞核心）的目标是招募IPF受试者，并对其进行表型分析。 临床、放射学和病理学特征; IPF组织标本的获取、处理、基因分型和储存 受试者和对照;并为本计划中的每个项目提供高质量的组织， 来自纤维化和正常肺的特定区域的细胞。我们将通过以下关键功能来实现这一点： 1.招募IPF受试者和对照 2.临床表型 3.样本采集、基因分型、储存和分发 4.来自不同功能区域的未传代和传代细胞（所有细胞类型）的分离和储存 IPF和对照肺。 5.不同功能的气道上皮细胞、肺泡上皮细胞和成纤维细胞的分离和储存 IPF区域和对照肺。 6.向项目分发组织、细胞和临床表型数据。 人体组织和细胞核心将作为人体组织所需资源的集中位置- 和细胞为基础的调查项目，因此是一个不可分割的综合组成部分的计划。 从广泛表型IPF研究的特定功能肺区域分离的人类组织和细胞 参与者是一种独特的资源，将有利于该计划中的所有三个项目， 树立政治意识